Category Archives: LAST

Infrared Laser Ablation Microsampling with a Reflective Objective

Dong, C., Richardson, L.T., Solouki, T., Murray, K.K.: Infrared Laser Ablation Microsampling with a Reflective Objective. J. Am. Soc. Mass. Spectrom. 33, 463-470 (2022). doi: 10.1021/jasms.1c00306 Abstract A Schwarzschild reflective objective with a numerical aperture of 0.3 and working distance of 10 cm was used for laser ablation sampling of tissue for off-line mass spectrometry. …

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Deep-ultraviolet laser ablation sampling for proteomic analysis of tissue

R.O. Lawal, L.T. Richardson, C. Dong, F. Donnarumma, T. Solouki, K.K. Murray, Deep-ultraviolet laser ablation sampling for proteomic analysis of tissue, Anal. Chim. Acta, 1184 (2021). doi: 10.1016/j.aca.2021.339021 Abstract Deep-ultraviolet laser ablation with a pulsed 193 nm ArF excimer laser was used to remove localized regions from tissue sections from which proteins were extracted for …

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Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer

A.C. Pulukkody, Y.P. Yung, F. Donnarumma, K.K. Murray, R.P. Carlson, L. Hanley, Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer, PLoS One, 16 (2021) e0250911, doi: 10.1371/journal.pone.0250911 Abstract Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth gives rise to changes in phenotype. There has been …

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MALDI imaging directed laser ablation tissue microsampling for data independent acquisition proteomics

K. Wang, F. Donnarumma, M.E. Pettit, C.W. Szot, T. Solouki, K.K. Murray, MALDI imaging directed laser ablation tissue microsampling for data independent acquisition proteomics, J. Mass Spectrom., 55 (2020) e4475; doi: 10.1002/jms.4475 Abstract A multimodal workflow for mass spectrometry imaging was developed that combines MALDI imaging with protein identification and quantification by liquid chromatography tandem …

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