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- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.6c00098
- Analytical ChemistryDOI: 10.1021/acs.analchem.6c00938
- Seed longevity is a key determinant of crop establishment, productivity, and germplasm conservation. During storage and germination, reactive oxygen species accumulate and contribute to seed aging through oxidative damage and loss of viability. The maintenance of redox homeostasis therefore relies on NADPH-dependent antioxidant systems, which require a continuous supply of reducing power. NADP-dependent malic enzyme 1 (NADP-ME1), represents a source of NADPH supporting antioxidant defense during seed aging. Here, we show that enhanced expression of NADP-ME1 positively contributes to seed […]
- ABSTRACT Rationale Pollution of surface and shallow groundwater by nitrate (NO3−$$ {\mathrm{NO}}_3^{-} $$) is a global concern, resulting in the deterioration of drinking water quality. Stable isotopes of NO3−$$ {\mathrm{NO}}_3^{-} $$ (δ15N and δ18O) can be used to trace its sources and identify prevailing biogeochemical processes. Methods Conversion of aqueous NO3−$$ {\mathrm{NO}}_3^{-} $$ to N2O headspace gas by Ti(III) reduction is a new method for analysis of NO3−$$ {\mathrm{NO}}_3^{-} $$ stable isotopes. Previous literature introduces the analytical procedure but provides […]
- The type VI secretion system (T6SS) is a contractile nanoweapon widely employed by Gram-negative bacteria to gain competitive advantages by injecting effector proteins into recipient cells. Although the biochemical activities of T6SS effectors have been well characterized, how recipient factors modulate effector toxicity remains poorly understood. Using Agrobacterium C58 as a model, previous work identified the Escherichia coli ClpAP protease as a recipient susceptibility (RS) factor that enhances T6SS-mediated interbacterial competition. Agrobacterium C58 deploys two DNase effectors, Tde1 and Tde2, […]
- Publication date: Available online 18 April 2026Source: Analytica Chimica ActaAuthor(s): Laura Carbonell-Rozas, Ane Arrizabalaga-Larranaga, Laura Righetti
- Journal of Proteome ResearchDOI: 10.1021/acs.jproteome.6c00007
- Publication date: Available online 17 April 2026Source: International Journal of Mass SpectrometryAuthor(s): Saphon Hok, Alexander K. Vu, David S. Cho, Carlos A. Valdez
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00438
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00272
- Mass Spectrometry Reviews, EarlyView.
- Mass Spectrometry Reviews, EarlyView.
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c08059
- Chemistry – A European Journal, Volume 32, Issue 14, 15 April 2026.
- Chemistry – A European Journal, Volume 32, Issue 14, 15 April 2026.
- Rapid Communications in Mass Spectrometry, Volume 40, Issue 14, 30 July 2026.
- ABSTRACT Rationale Food fraud, particularly meat adulteration, poses risks to consumer trust, public health, and regulatory compliance. Existing detection methods are often slow and require complex preparation. There is a need for rapid, reliable, and accessible analytical approaches to verify meat authenticity in both laboratory and field settings. Methods Atmospheric Solid Analysis Probe Mass Spectrometry (ASAP-MS) was used for direct analysis of meat samples with minimal preparation. Spectral data from seven meat species were processed using chemometric modelling to build […]
- The armadillo repeat protein ARMH3 regulates the activity and localisation of the Golgi resident lipid kinase phosphatidylinositol 4 kinase III{beta} (PI4KB) and the Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1) that activates Arf1. ARMH3 localises to the trans Golgi network (TGN) via the GTPase Arl5. We used hydrogen deuterium exchange mass spectrometry (HDX-MS) and AI-enabled modeling to define the interfaces of ARMH3 with its binding partners Arl5, PI4KB, and GBF1. The ARMH3-Arl5 interface was determined to consist of […]
- Aging at the subcellular level involves the simultaneous decline in the cell's ability to maintain protein homeostasis and rise in misfolded proteins through a positive feedback loop. Here, we test if a widespread class of protein misfolding could contribute to proteome aging by examining if statistical associations exist between age-related changes in protein structure, measured by limited proteolysis mass spectrometry data of the aging Saccharomyces cerevisiae proteome, with structural annotations and molecular simulations. We find that globular proteins that are […]
- Journal of the American Chemical SocietyDOI: 10.1021/jacs.5c20644
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.6c00088
- As part of the US HUPO sponsored "All the Parts", Ben and Ben sat down to talk with Dr. Sasha Singh, Harvard Medical School, and Dr. Sarah Parker, Cedars-Sinai (also featured in Ep 15).keywords: hEDS; POTS; valves; foamy macrophages; atherosclerosis; aneurysm
- ABSTRACT The global prevalence of gout continues to rise. Baxian Huazhuo Decoction (BHD) has demonstrated significant efficacy in the clinical treatment of acute gouty arthritis (AGA); however, its mechanism of action remains unclear. This study first employed network pharmacology analysis to identify the key components, targets, and pathways of BHD against AGA. Molecular docking studies validated the binding affinity between the components of BHD and their potential targets. Ultrahigh-performance liquid chromatography–high-resolution mass spectrometry (UHPLC–HRMS) was utilized to identify the active […]
- Analytical ChemistryDOI: 10.1021/acs.analchem.6c01350
- A previously overlooked class of protein entanglements, non-covalent lasso entanglements (NCLEs), has been found to play a role in widespread protein misfolding. However, understanding the influence NCLEs have on biological processes is hindered by the absence of dedicated algorithms and computational tools to detect and characterize these geometries in protein structures, molecular dynamics simulations, and in comparison to experimental data from limited proteolysis (LiP) and cross-linking (XL) mass spectrometry (MS). Here, we present EntDetect, a software tool designed to: (1) […]