Combined RSS Items
- ABSTRACT Intensive farming practices have necessitated the extensive use of veterinary drugs, raising significant concerns regarding residue contamination. Consequently, long-term monitoring of residues and the evaluation of dietary exposure risks are essential. In this study, 326 pork samples were collected from local supermarkets between October 2024 and April 2025. Methods were developed for the analysis of 25 veterinary drugs (including sulfonamides, tetracyclines, fluoroquinolones, β-agonists, amphenicols, macrolides, and roxarsone) using high-performance liquid chromatography-tandem mass spectrometry. Roxarsone was extracted using a methanol/water […]
- A framework for the exploration of subcellular compartmentalization of RNA-binding proteins
- PROTEOMICS, EarlyView.
- Host defense peptides (HDPs) are important components of the innate immune system that are used to combat pathogens and often rely on metal binding for their function. However, controlling trace nutrients such as transition metals may have other roles in host-symbiont interactions beyond poisoning harmful pathogens. This study characterizes the evolution, structural properties, and biochemical activity of the novel hymenopteran HDP abaecin-2. In myrmicine ants such as the fungus-growing tribe Attini, abaecin-2 has evolved to include an Amino-Terminal Cu(II) and […]
- Insects' body barriers rely on specialized extracellular matrices that protect against harmful environmental influences. The outer barrier is the cuticle, which is composed of chitin, cuticle proteins and lipids. The peritrophic matrix (PM) serves as an inner barrier lining the midgut epithelium. It is composed of chitin fibers that are organized by PM proteins. While cuticle and PM proteins have received considerable attention in the past, supramolecular organization and physicochemical properties of the chitin component – particularly of the PM […]
- Endosymbiosis of phytoplankton in heterotrophic hosts is ecologically important and has led to key evolutionary innovations. However, the dynamic molecular processes underlying endosymbiosis establishment remain poorly understood. Here, using large-particle sorting and liquid chromatography-tandem mass spectrometry, we unravel heterogeneous changes in proteomes of the cosmopolitan ciliate Paramecium and algal endosymbiont Chlorella from engulfment to stable endosymbiosis. The initial digestion sees a sharp decline of intracellular Chlorella cells, along with host cellular reorganization involving a reduction of the cortex-localized defensive organelles, […]
- Plants produce diverse bouquets of specialized metabolites (SMs), yet only a fraction of the vast phytochemical space has been explored to date. Comparative analysis of SM profiles can reveal hotspots of biochemical novelty, while systematic profiling across taxonomic levels does presently not cover large plant families. To study core and accessory SM profiles in the Brassicaceae plant family, we fingerprinted 14 species by Liquid-Chromatography Mass-Spectrometry (LCMS/MS). We develop standardized experimental and computational workflows integrating in silico annotation tools to study […]
- J. Anal. At. Spectrom., 2026, Advance ArticleDOI: 10.1039/D6JA90018F, Atomic Spectrometry UpdateE. Hywel Evans, Jorge Pisonero, Clare M. M. Smith, Rex N. TaylorThis review covers advances in atomic spectrometric techniques, including atomic emission, absorption, fluorescence and mass spectrometry. Material on speciation and coupled techniques is not covered as this is included in a separate ASU review.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of […]
- Human Fc receptor-like 5 (FCRL5) is a low-affinity IgG Fc receptor expressed on various B cell subsets and a potential therapeutic target. We discovered that commonly used Fc-silencing mutations, designed to prevent interactions between the Fc{gamma} receptors on immune cells and the Fc domain of therapeutic IgG, do not prevent binding to FCRL5. As a result, unintended interactions between Fc-silent therapeutic IgG and human B cells may occur. We isolated a well-expressed variant of the Fc-binding portion of human FCRL5 […]
- ABSTRACT Analytical technologies for body fluids and tissues have advanced substantially over the past decade, particularly in chromatographic, electrophoretic and ion-mobility–based separation strategies designed to address matrix complexity and structural isomerism. Improvements in stationary-phase chemistry, multidimensional liquid chromatography, capillary electrophoresis and mobility-integrated mass spectrometry have enhanced peak capacity, structural discrimination, remains the selection and validation of separation strategies capable of minimizing coelution, ion suppression and quantitative variability in heterogeneous matricesremains the selection and validation of separation strategies capable of minimizing […]
- ABSTRACT Rationale Impurity profiling is critical for the quality control and safety assessment of pharmaceutical formulations. Tolterodine tartrate tablets contain unknown impurities that are not documented in standard literature or pharmacopoeias. Identifying these impurities and determining their origin is essential for optimizing the manufacturing process and ensuring product stability. Methods A comprehensive two-dimensional liquid chromatography-mass spectrometry (2D-LC/MS) method was developed. The first-dimension separation used a Waters XBridge C18 column (4.6 × 250 mm, 5 μm) with gradient elution (mobile phase A: acetonitrile/water/perchloric acid, 200:800:1.5, […]
- ABSTRACT Rationale Asplenium ruprechtii Sa. Kurata (“Guo shan jue”), a traditional Chinese folk medicine, is widely used to treat thromboangiitis obliterans, hemiplegia, traumatic bleeding, neurodermatitis, and lower limb ulcers. However, its chemical composition remains incompletely characterized—previous studies have identified 80 major components, while trace-level constituents (critical for the “multicomponent, multitarget” efficacy of traditional Chinese medicine) remain unexploited. Methods In this paper, a high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-Q-TOF-MS/MS)-based molecular networking was used to explore the […]
- Background: Tau aggregation is the defining feature of tauopathies, however, the mechanisms by which distinct tau strains drive disease-specific responses remain unclear. Existing models largely rely on recombinant tau seeding or tau overexpression, which fail to capture the biochemical diversity of pathological tau. The aim of this study was to develop a robust and reproducible human cell-based model of disease-specific tau pathology and to use this model to determine how tau from unique diseases impact tau accumulation and lysosomal dysfunction. […]
- Publication date: Available online 20 April 2026Source: Clinica Chimica ActaAuthor(s): Zhonggan Jin, Yuanzhu Ou, Ming Zong, Zhihan Ye, Zhizheng Li, Sujie Zhang, Hewei Sun, Xiaoyu Fan, Xialin Luo, Lingli Jiang, Yi Ju
- Publication date: Available online 19 April 2026Source: Clinica Chimica ActaAuthor(s): Lei Wang, Xingli Xu, Hongchen Zhou, Feng Zhang, Huahui Li
- Publication date: Available online 20 April 2026Source: TalantaAuthor(s): Qianlin Wang, Danmei Zhao, Guohui Xing, Conghui Sun, Yifan Cai, Nian Liu, Linnan Ke, Lihong Liu, Bin He, Ligang Hu
- An alternative to the conventional hydrogenation pathway for NO3 − remediation is established via HCOOH-driven proton-coupled electron transfer on a bimetallic Sn3/Pd catalyst. The triangular Sn3 ensembles thermodynamically favor direct N─N coupling while kinetically suppressing N─H formation, achieving 97.4% N2 selectivity. This design bypasses the conventional hydrogenation route, overcoming the longstanding NH3 byproduct challenge. ABSTRACT Catalytic nitrate (NO3 −) reduction (CNR) to dinitrogen (N2) offers an efficient strategy for remediating nitrogen pollution but is constrained by preferred ammonia (NH3) formation. […]
- Publication date: July 2026Source: TrAC Trends in Analytical Chemistry, Volume 200Author(s): Xinying Zhu, Jia Yi, Liang Qiao
- Glycan biosynthesis relies on nucleotide-activated sugars, essential metabolites across all domains of life, yet their usage in microbes is poorly understood. Here we present SugarBase, a mass spectrometry and bioinformatic pipeline for untargeted exploration of microbial nucleotide sugar networks. SugarBase resolves the chemical complexity of microbial metabolism by combining narrow-window DIA fragmentation with a chemistry-informed parent ion identification algorithm. Applying SugarBase across a broad phylogenetic range of microbes revealed extensive, species-specific nucleotide sugar profiles, including many candidates with no existing […]
- In mass spectrometry-based proteomics, false discovery rate (FDR) control underpins the credibility of peptide and protein identifications. In contemporary workflows, including multi-run Data Independent Acquisition (DIA), deep learning-assisted scoring, library-free searches, and extensive post-processing, the statement "1% FDR" has become increasingly ambiguous, potentially referring to different statistical entities, multiple-testing scopes, and null models. We propose a standardized framework requiring explicit specification of three complementary properties: "scope", meaning which statistical universe is controlled; "calibration", meaning whether confidence measures behave consistently with […]
- Per- and polyfluoroalkyl substances (PFAS) are highly resistant to enzymatic C-F bond cleavage, and hydrolytic defluorination of long-chain PFAS has rarely been demonstrated. Here, we report selective hydrolytic defluorination of branched perfluorooctanoic acid (PFOA) isomers by a haloacid dehalogenase (4A) from Delftia acidovorans strain D4B. A fluoride-specific riboswitch biosensor was used for initial substrate screening, followed by scaled-up assays in which fluoride release was quantified using a fluoride ion-selective electrode. Defluorination products were subsequently identified by liquid chromatography-mass spectrometry (LC-MS). […]
- The inner nuclear membrane protein SUN1 regulates cullin-3 neddylation to maintain insulin signalingMetabolic dysfunction-associated steatotic liver disease (MASLD) is the most prevalent chronic liver disease and strongly linked to obesity and insulin resistance. We previously reported that the common nuclear envelope variant rs6461378 (g.842031C>T; SUN1 H118Y) associated with MASLD and related traits including insulin resistance. To gain insight into how wildtype (WT) and H118Y SUN1 might differentially impact insulin signaling, we performed affinity purification-mass spectrometry (AP-MS) in human liver-derived cells stably expressing WT or H118Y SUN1. Unbiased AP-MS revealed a novel SUN1-CUL3 […]
- A cold semi-swap
- Structure-informed deep generation enables de novo metabolite annotation in untargeted metabolomics
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.6c00098