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- Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease whose diagnosis often remains delayed. Skeletal muscle is increasingly recognized as an early contributor to ALS pathology. Using lipid imaging mass spectrometry (LIMS) in Tibialis anterior muscle from hSOD1G93A mice across disease stages, we identified fiber-type-specific and sex-dependent lipid remodeling. Lipid alterations were detected at the presymptomatic stage, preceding motor neuron loss and clinical symptoms. LIMS distinguished fast-twitch oxidative-glycolytic (type IIA) and glycolytic (type IIB/IIX) fibers and revealed their differential vulnerability […]
- Publication date: Available online 12 July 2026Source: Journal of ProteomicsAuthor(s): S. Wislet, C. Le Goff, Massonet Ph, S. Hody, J.L. Croisier, V. Dâorio, J.F. Kaux, G. Mazzucchelli, R. Cloots
- Publication date: Available online 11 July 2026Source: Journal of ProteomicsAuthor(s): Dirk Winkelhardt, Julia P. Schessner, Sven Berres, Alireza Nameni, Armin Soleymaniniya, Ayla SchrĂśder, Caroline Jachmann, Douwe Schulte, Georg Wallmann, Henry Webel, Johannes Rainer, Katerina Nastou, Laurent Gatto, Magnus Palmblad, Maike Weber, Omar Shouman, Pavel Sinitcyn, Ralf Gabriels, Robbe Devreese, Tim Van Den Bossche
- With the rapid development of mass spectrometry-based proteomics, the volume of phosphoproteomic data has increased substantially. However, accurate localization of phosphorylation sites and standardized statistical validation remain critical analytical bottlenecks. To address the lack of standardized cross-algorithm evaluation, we introduce onsite, a unified and open-source Python framework. onsite integrates an alanine-decoy strategy to estimate the false localization rate (FLR) across three algorithms: AScore, PhosphoRS, and pyLucXor. This modular architecture efficiently processes large-scale datasets and enables global FLR calculation. Benchmarking on […]
- Heterozygous de novo missense mutations in the EEF1A2 gene encoding translation elongation factor eEF1A2 result in neurodevelopmental disorders, typically characterised by early onset epilepsy and intellectual disability (ID). The E122K mutation is the most commonly reported missense mutation and is amongst the more severe in terms of epilepsy and ID. Here we made use of a recently developed mouse model which recapitulates the E122K mutation to examine how mutations in EEF1A2 might disrupt neuronal gene expression. Primary neurons from mutant […]
- ABSTRACT Loureirin D is a bioactive dihydrochalcone isolated from Dracaena resin with very high therapeutic value. A sensitive and high-throughput ultra-performance liquid chromatographyâmass spectrometry (UPLCâMS/MS) method was developed for the quantitative analysis of loureirin D in rat plasma with only 50âÎźL of sample per assay. Sample extraction was protein precipitation using acetonitrile, and isoscoparin was used as internal standard (IS). Separation was achieved on the BEH C18 column with a gradient mobile phase of 0.1% (v/v) aqueous formic acid and […]
- ABSTRACT SHR6508, a novel calcimimetic agent, is currently being developed for treating secondary hyperparathyroidism in chronic kidney disease (CKD) patients requiring maintenance hemodialysis. For the purpose of quantifying SHR6508 in human plasma, a sensitive and reliable LCâMS/MS method was first developed and successfully validated. Since SHR6508 is a disulfide bond-containing peptide, ascorbic acid was supplemented into blood samples during collection to prevent thiol-disulfide exchange reactions. Plasma matrix samples were processed by protein precipitation with a waterâacetonitrile solution (1:4, v/v) containing […]
- Hot chemistry in cold ices reveals the previously elusive sulfinothioic acid molecule, HS(O)SH, prepared through nonequilibrium chemistry in low-temperature ices and identified with isomer selective photoionization reflectron time-of-flight mass spectrometry combined with isotopic labeling. ABSTRACT Low-oxidation-state sulfur oxyacids are key yet experimentally elusive intermediates in interstellar sulfur chemistry; even the simplest member, sulfinothioic acid (HS(O)SH), has neither been detected in space nor isolated terrestrially. The SâS(O) motif, also present in biological systems, can be reduced to form disulfide bridges (RSSRâ), […]
- Publication date: October 2026Source: TrAC Trends in Analytical Chemistry, Volume 203Author(s): Wen Li, Bin Hu
- This study investigated site-specific N-glycosylation alterations in thyroglobulin from patients with papillary thyroid carcinoma using nano-LCâMS/MS. Advanced bioinformatics and machine learning identified these unique glycan signatures, establishing the Asn1365-HexNAc(3)Hex(6) glycoform as a highly predictive diagnostic biomarker (AUCÂ =Â 0.83). ABSTRACT Thyroglobulin (Tg) is a clinically established biomarker for thyroid cancer; however, its diagnostic specificity remains limited due to confounding benign conditions. This study investigated changes in the site-specific N-glycosylation profiles of thyroglobulin protein derived from papillary thyroid cancer and adjacent healthy tissues. […]
- Natural compound phosphonothrixin's herbicidal activity resides exclusively in its (S)-enantiomer, yet existing enantioselective syntheses are cumbersome, and direct chiral LC separation is hampered by the compound's high polarity, lack of aromatic groups, and metal chelationâinduced peak tailing. By employing a zwitterionic sulfohexyl quinidine carbamate, stationary phase reliable analytical and preparative chiral separations were achieved, enantiomers isolated, and absolute configurations assigned by optical rotation and ECD spectroscopy. ABSTRACT Phosphonothrixin (PPX), a phosphonate natural product known for its potent herbicidal activity, has […]
- Journal of Mass Spectrometry, Volume 61, Issue 8, August 2026.
- This study established the first comprehensive pharmacokinetic profile of Mailuoning Oral Liquid by tracking 39 compounds in rats, identifying 15 key systemic markers via UHPLC-HRMS and validated UHPLC-MS/MS assays. Dose-dependent absorption was observed, providing an evidence base for dose refinement and clinical application. ABSTRACT Mailuoning oral liquid (MLN) is a state-approved multi-herb formula that has been prescribed for 25 years to clear heat, nourish yin, invigorate blood, and resolve stasis. Although randomized controlled trials and real-world evidence support its efficacy […]
- ABSTRACT Solanum americanum Mill. and S. nigrum Linn. are traditional Solanaceae-derived herbal medicines. Their sprouts and mature fruits are utilized as both vegetables and medicinal herbs. Although S. americanum Mill. is classified as a primary variety of S. nigrum Linn., significant pharmacological variations and incompletely characterized phytochemical profiles differentiate their applications. In this study, UHPLC/LTQ-Orbitrap mass spectrometry (HRMS) (Thermo Fisher Scientific, USA), assisted with feature-based molecular networking, was applied for in-depth profiling of S. americanum Mill. and S. nigrum Linn. […]
- ABSTRACT Pyrrolizidine alkaloids (PAs) and their N-oxides (PANOs) are naturally occurring toxic plant metabolites that have attracted widespread attention due to their potential food safety hazards. In this study, an efficient analytical method for determining PAs/PANOs in bee pollen was established by combining accelerated solvent extraction (ASE) with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A magnetic covalent organic framework material (Fe3O4@COF-TAPB-TFP) was synthesized and used as an adsorbent for offline purification to reduce matrix interference and improve detection sensitivity. […]
- ABSTRACT Caffeoylquinic acids are natural polyphenolic compounds with notable antioxidant and anti-inflammatory activities. This study developed and validated a sensitive high-performance liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of isochlorogenic acid A (ICQA) and 22 metabolites in serum. The method exhibited excellent sensitivity with limits of quantification of 0.625â1.25 Âľg/L (signal-to-noise ratio = 10), inter/intra-day precision below 15%, and comprehensive coverage of phase I/II and microbial metabolites. Application of the method in rats and laying hens demonstrated its […]
- ABSTRACT Quantifying hydrophilic B vitamins (B1 [(thiamine], B2 [riboflavin], and B3 [nicotinic acid]) in complex functional food matrices presents a significant challenge. Conventional sorbents, such as C18, often exhibit poor retention and low recoveries (50%â60%) due to weak hydrophilic interactions. To efficiently extract these target analytes from functional foods, a new sorbent with tailored hydrophilic affinity has been developed by incorporating Co-cyromazine complexes onto halloysite nanotubes. This composite was employed as the sorbent for a spin-tip solid-phase extraction method, which […]
- Are you interested in getting into spatial mass spectrometry but all the super expensive commercial technologies aren't doing it for you? Why don't you…..introducing Tappy-mode scanning ESI! What's that? Okay, they didn't invent it for this paper, but they explain it better than I can! Wait a minute. Is it just like this except really fast and with an electrospray plume in the middle? Close enough! Okay, but analogies aside, we can move tissues at incredibly absurdly high resolutions. Think about the stages they use […]
- Background: Fragile X-associated Tremor/Ataxia Syndrome (FXTAS) is a late-onset neurodegenerative disorder caused by FMR1 premutation CGG repeat expansions (55-200 repeats). The epigenetic landscape of the FXTAS brain remains uncharacterized. We performed genome-wide DNA methylation profiling of postmortem prefrontal cortex tissue to identify differentially methylated positions (DMPs) and candidate genes, and sought protein-level support for a neuroinflammatory signal. Methods: DNA methylation was profiled in postmortem prefrontal cortex (Brodmann area 9) from 27 male FXTAS cases and 29 male controls using the […]
- Cells release membrane-bound extracellular vesicles into the bloodstream laden with proteins that may reflect their physiological state. How this circulating EV proteome changes across life remains poorly understood. Identifying molecular signatures of aging in accessible biofluids could facilitate earlier intervention and monitoring of age-related disease. Many circulating aging proteome studies rely on affinity-based platforms which suffer from poor cross-species translation, ambiguous signal attribution, and inconsistent agreement between platforms. Here, we present a characterization of the aging plasma EV proteome from […]
- Endometriosis, a common inflammatory gynecological disorder affecting up to 10% of women worldwide, is characterized by the presence of endometrium-like tissue outside the uterus. Current diagnostic methods, such as ultrasound and MRI, effectively detect ovarian and deep endometriosis but fail to detect more common peritoneal type. Diagnosing peritoneal endometriosis currently necessitates invasive laparoscopy and histological confirmation. Despite numerous efforts, no new reliable biomarkers have successfully transitioned into routine clinical use. This study aimed to investigate the use of targeted metabolomics […]
- Regeneration of skeletal muscle preserves muscle mass and function, which decline with age. Here, we sought to identify long noncoding (lnc)RNAs involved in skeletal muscle myogenesis and potentially relevant to muscle aging. Cross-sectional analysis of skeletal muscle transcriptomes from healthy 22-through 89-year-old individuals revealed lncRNA LANCL1-AS1 among the top declining transcripts. Conversely, LANCL1-AS1 increased robustly during skeletal myogenesis and promoted myogenic differentiation in culture. Affinity pulldown by ChIRP followed by mass spectrometry revealed that LANCL1-AS1 associated with the mitochondrial protein […]
- Publication date: 1 January 2027Source: Talanta, Volume 311Author(s): Wenjing Ma, Marwan Alajlouni, Dustin Schrecongost, Sa Liu, Robert E. Synovec
- ABSTRACT Rationale Food classification and adulteration detection are challenged by the complexity of chemical matrices. Traditional descriptor-based methods fail to capture interactions in chromatographyâmass spectrometry (CMS) data. This study compares a convolutional neural network (CNN) against a random forest (RF) baseline using engineered descriptors to classify 15 food items to demonstrate deep learning advantage for objective, scalable food authentication. Methods Three thousand CMS spectra is computationally simulated (200 per class) based on FooDB compound profiles. Six engineered descriptors (mean intensity, […]
- ABSTRACT Rationale In forensic investigations, questioned document examination (QDE) provides critical information for document authentication and the detection of forgeries. Conventional QDE approaches often require separate analyses of inks and of any potential fingerprints. This study compares two mass spectrometry imaging (MSI) techniques for QDE, enabling the simultaneous analysis of inks and fingerprints deposited onto paper, providing chemical and biometric information from a single sample. Methods Two mock forgery samples containing two ballpoint pen inks, printed ink and latent fingerprints […]