Combined RSS Items
- ABSTRACT Rationale In the development of quadrupole mass spectrometers, there is a growing interest in understanding the modified first stability region under conditions of unbalanced DC and RF voltages. Methods Based on the properties of the solution to the Dirichlet problem for the Laplace equation, the potentials on the orthogonal rod pairs of a quadrupole mass filter were determined. The trajectory method was employed to calculate the stability regions and transmission contours. Results The equations of ion motion were derived […]
- ABSTRACT Rationale Insomnia is a highly prevalent disorder with substantial health and socioeconomic impacts, for which traditional Chinese medicine is an important treatment option. Jieyu Anshen Granule (JYAS-G) shows excellent clinical efficacy in insomnia treatment, but its active components, inĀ vivo migration characteristics and underlying mechanisms remain unclear. Methods Two mouse insomnia models induced by p-chlorophenylalanine (PCPA, chemical method) and sleep deprivation machine (SDM, physical method) were established respectively, and mice were administered with low and high doses of JYAS-G, with […]
- AbstractHyperphosphorylation of tau is central to the development and progression of Alzheimerās disease (AD) and other tauopathies. How different kinases and their phosphorylated tau products, as well as additional post-translational modifications (PTMs) of tau, contribute to AD is a critical yet poorly understood question. We created a comprehensive landscape of PTMs (i.e., phosphorylation, acetylation, and methylation) of tau across isoforms (2N4R, 1N4R, and 0N3R) and kinases (GSK3Ī², TTBK1, and DYRK1A) using complementary capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) and […]
- AbstractCorynebacterium glutamicum serves as a pivotal industrial platform organism, accounting for an annual global amino acid output of several million tons. Elucidating its adaptive mechanisms under diverse environmental perturbations during high-density fermentation is fundamental to advanced metabolic engineering and strain optimization. Despite the critical role of protein phosphorylation in cellular adaptation, the phosphoproteome of C. glutamicum remains largely unexplored. With fewer than 50 phosphoproteins currently identified, this knowledge gap hinders the elucidation of regulatory networks underlying industrial stress tolerance. In […]
- AbstractHost cell protein (HCP) characterization in monoclonal antibody (mAb) products is challenged by a large concentration gap between low-abundance HCPs and high-abundance mAb proteins. Advances in sample preparation, chromatography, and mass spectrometry techniques have aimed at either reducing this concentration imbalance or the peptide coelution effects during chromatography. Despite this, comprehensive characterization of HCPs remains difficult, particularly at below-ppm levels, often requiring complex and low-throughput methodologies. This study presents a rapid, sensitive, and high-throughput HCP characterization workflow integrating the Seer […]
- AbstractAlthough the carbohydrate biomarker CA19-9 is widely used for cancer screening, its diagnostic specificity is severely compromised by false-positive elevations in benign inflammatory conditions. Characterizing specific CA19-9 protein carriers provides a promising alternative, yet high-throughput, micro-volume profiling directly from serum remains a challenge. Here, we present a 96-well chemical proteomic platform for the high-throughput profiling of CA19-9-modified proteins. Utilizing a tailored trifunctional chemical probe, photo-activated covalent crosslinking between anti-CA19-9 antibodies and target carriers enables high-stringency enrichment within streptavidin-coated microplates. The […]
- Ā THE iSCMS/BMSS Proteomics Show is live! Link here!Ā Wait. Did the Spotify AI do something to our faces? I think it smoothed our faces. You get what you pay for, I guess.Ā While unjetlagging myself I felt silly about things I forgot about iSCMS, especially some thank you!Ā Thank you Proteomes! for the editor travel support. First time I've used that perk of being an editor.Ā Like I said on stage, the impact factor of all the core proteomics journals suck. So […]
- AbstractThe association of organic carbon (OC) with iron (Fe) minerals is a primary mechanism governing soil OC persistence, yet the molecular agents mediating FeāOC binding remain poorly defined. In this study, we demonstrated that microbial degradation of lignin, one of the major contributors to soil OC, generates organic compounds with the potential to bind to Fe, with implications for mineral-associated carbon stabilization. Lignin samples were microbially degraded by Pseudomonas putida KT2440 for 30 days and subsequently reacted with FeCl3. Ultrahigh-performance […]
- A special live show from the 2026 International Single Cell Mass Spectrometry Conference (iSCMS) in London. Ben Orsburn was joined by special co-host Prof. Melanie Bailey from Kingās College London, and they sat down to talk to Professor Edward Emmott, University of Liverpool.
- A bioorthogonal vitamin C probe platform enables visualization, enrichment, and profiling of lysine vitcylation in living cells. Chemoproteomics, imaging, and CRISPR screening reveal a spatially organized vitamin C-derived protein modification landscape and identify GSTP1 as a regulator of this modification. This strategy provides a chemical framework for exploring metabolite-derived protein regulation. ABSTRACT Vitamin C is an essential micronutrient, yet its direct chemical engagement with the proteome remains poorly defined. Here, we develop and experimentally benchmark a bioorthogonal probe platform for […]
- AbstractFragment crystallizable (Fc)-fusion proteins are formed by the fusion of functional peptides or receptor extracellular domains (ECDs) with Fc. They are normally very heterogeneous with multiple N- and O-glycosylation sites and a high level of sialylation. Characterization of O-glycosylation in Fc-fusion proteins is important for understanding their biological significance and for monitoring process consistency during drug development. However, due to the heterogeneity, close localization, and especially low ionization efficiency of O-glycans, it is challenging to achieve the overall O-glycosylation profile […]
- Publication date: Available online 13 October 2026Source: TalantaAuthor(s): Jun Lai, Yan Zhang, Yun-Peng Zhao
- Publication date: Available online 12 October 2026Source: TalantaAuthor(s): Ziyue Ma, Jianbin Liu, Simon Sani Ocholi, Zixin Zhang, Liming Wang, Shin Sik Choi, Fengchao Wang, Baiping Ma, Lifeng Han
- Black carbon (BC) particles are a health-relevant component of combustion-derived air pollution, but internal exposure remains difficult to assess in biological samples. Existing biomonitoring approaches detect or count BC particles but provide limited information on particle mass or source-resolved chemical fingerprints. We reconstructed benzene polycarboxylic acid (BPCA) molecular-marker analysis into a small-volume workflow combining ambient nitric-acid digestion, nanoparticle enrichment, organic-interference removal, single-step solid-phase extraction, and gas chromatographyāmass spectrometry (GCāMS). Orthogonal partial least-squares regression quantified BC mass, and a hierarchical OPLS […]
- Megadalton biomolecular assemblies, including viral vectors, synthetic nanoparticles and protein aggregates, are increasingly important chemical and therapeutic entities, yet their heterogeneity often exceeds the resolving power and space charge tolerance of conventional mass spectrometry. To overcome these limitations, here we report the first realisation of a Poschenrieder dual-sector electrostatic analyser as a charge-detection mass spectrometer. The instrument traps individual ions in a continuous closed trajectory between two spherical electrostatic sectors, enabling induced-current detection over extended transients without end-cap deceleration. Coupled […]
- Publication date: Available online 12 October 2026Source: International Journal of Mass SpectrometryAuthor(s): Manxi Yang, Xindi Tang, Julia Laskin
- Publication date: Available online 12 October 2026Source: International Journal of Mass SpectrometryAuthor(s): Marta Kowalska-Jachimek, Robert Wieczorek, Piotr Stefanowicz, Remigiusz BÄ chor
- Publication date: Available online 12 October 2026Source: TrAC Trends in Analytical ChemistryAuthor(s): Laurine Lagache, Michel Salzet, Isabelle Fournier
- Journal of Mass Spectrometry, Volume 61, Issue 11, November 2026.
- Journal of Mass Spectrometry, Volume 61, Issue 11, November 2026.
- Journal of Mass Spectrometry, Volume 61, Issue 11, November 2026.
- Journal of Mass Spectrometry, Volume 61, Issue 11, November 2026.
- ABSTRACT Objective This study aimed to investigate the mechanism and pharmacologically active compounds of Fufang Ejiao Jiang (FFEJJ) in the prevention and treatment of myelosuppression after chemotherapy (MAC) through a combination of serum metabolomics and network pharmacology. Methods Sprague Dawley rats were randomly assigned to three groups: the blank group, the model group, and the FFEJJ group (10.8āg/kg). A MAC rat model was induced using cyclophosphamide. Simultaneously with model establishment, rats were intragastrically administered once daily for 15 consecutive days. […]
- AbstractDetermining the three-dimensional structures of small molecules in complex mixtures remains challenging as current approaches typically require physical isolation and quantities of material that are excessive for many components. Here we present a reciprocal structure-determination strategy that integrates ion mobility-resolved high-resolution tandem mass spectrometry (IMS-HRMS-MS/MS) with microcrystal electron diffraction (MicroED/3DED), enabling chemically consistent three-dimensional structure elucidation from sub-microgram quantities of material. Analysis of synthetic reaction mixtures revealed multiple stereoisomeric products with distinct ion-mobility and fragmentation behaviour. Mechanistically grounded modelling of […]
- Characterization of naturally processed MHC class I (MHC-I)-bound peptides is essential for defining allele-specific peptide-binding features and understanding the functional consequences of MHC-I polymorphism. However, immunopeptidome analyses of dog leukocyte antigen (DLA) class I (DLA-I) molecules remain limited and comprehensive monoallelic ligand datasets for prevalent DLA-88 alleles are still lacking. Here, we established canine melanoma-derived cell populations expressing individual functional DLA-88 alleles and generated a monoallelic immunopeptidome atlas of prevalent DLA-88 alleles. Using liquid chromatography-tandem mass spectrometry, we identified 32,702 […]