Combined RSS Items
- Over the last two decades, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) has become the standard method for identifying bacteria and has found a wide range of applications, especially in clinical microbiology. The method's high taxonomic resolution, minimal sample preparation, and complete, ready-to-use commercial systems, which include instrumentation, experimental protocols, spectral databases, and identification analysis software, were key factors in the success of MALDI-ToF MS as the standard for identifying microorganisms in routine diagnostic laboratories. However, despite the availability […]
- Journal of the American Chemical SocietyDOI: 10.1021/jacs.6c01876
- I received some questions about my longer term impressions of the amazingly sensitive and somewhat fast TIMSTOF Ultra2. It's been a year already? Yikes. I guess I'll reference my earlier TIMSTOF reviews.Great stuff!1) The sensitivity of this thing is still absolutely unreal. Multiple people are writing up papers on single cells ran on it with DIA label free and the numbers are cell type specific and amazing.Even with a high loss, but very inexpensive and very fast sample prep, most cancer […]
- Publication date: Available online 12 May 2026Source: Journal of ProteomicsAuthor(s): Isabel Cerro-Pardo, Amaiur Mendizabal, Mikel Azkargorta, Félix Elortza, Iñaki Rodríguez-Agirretxe, Arantxa Acera
- In many oviparous animals, egg yolk is the sole source of nutrition until feeding begins, and carbohydrates are present in only small amounts in the yolk. Glucose plays an important role in the developmental processes of various animals. In addition, gluconeogenesis has been reported to occur in the yolk syncytial layer (YSL) of cartilaginous fish and teleosts. In contrast, the role of gluconeogenesis in tetrapods remains unclear. In this study, we used Xenopus tropicalis, an anuran amphibian, which lacks YSL, […]
- Analytical ChemistryDOI: 10.1021/acs.analchem.6c00933
- ABSTRACT Cyclodextrin-based chiral stationary phases (CSPs) have attracted considerable interest in the field of enantiomeric separation due to their chiral recognition properties. However, the design and preparation of cyclodextrin-based CSPs with satisfactory application prospects are necessary for further exploration. Herein, we report an effective method for fabricating β-cyclodextrin-derived CSP by chemical bonding of 3,5-dimethylphenylcarbamate-mono-6-ethylenediamine-β-cyclodextrin (i.e., DMPCCB) onto silica microspheres via the diamine linker. After structure characterization, the performances in enantiomeric separation were assessed using chiral drugs, including proton pump inhibitors, […]
- ABSTRACT Shenzhuo Decoction (SZT), a classical traditional Chinese medicine (TCM) formula from Synopsis of the Golden Chamber (Jinkui Yaolue), demonstrates clinical efficacy in treating ankylosing spondylitis (AS); however, its systematic chemical profiling remains insufficient. This study established an integrated strategy for chemical constituent analysis of SZT, combining iterative ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF MS/MS) with multidimensional data annotation. The iterative UHPLC-Q-TOF MS/MS technology employed 20 eV/40 eV dual collision energies, along with three iterative scans and […]
- ABSTRACT Balsamic vinegars produced under Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI) schemes differ substantially in raw materials, processing intensity, and aging regimes. Although these differences are expected to shape their volatile composition, a comprehensive chemical characterization capable of reliably discriminating the two denominations remains analytically challenging. In this study, headspace solid-phase microextraction combined with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (HS-SPME-GC×GC-TOFMS) was used to characterize PDO and PGI balsamic vinegars and to evaluate the feasibility of […]
- ABSTRACT Ziyu ointment (ZYO) is a commonly used, approved ingredient in traditional Chinese medicine (TCM) medication for treating superficial swelling and ulcers. However, due to limited methodologies, more accurate and selective qualitative and quantitative analysis methods for its active ingredients have not yet been established. This impedes both the full clinical development and application of ZYO as well as the clarification of its pharmacological mechanism. In this research, a rapid and sensitive UHPLC-Orbitrap Exploris MS method with higher-energy collisional dissociation […]
- PROTEOMICS, EarlyView.
- Human quinone reductase 2 (QR2, NQ02) is a cytosolic flavoprotein involved in cell physiology and metabolism, and implicated in several diseases. However, the mechanisms that govern its oligomeric assembly and diverse functional outcomes remain incompletely understood. Here, we employ native mass spectrometry to directly resolve the dynamic oligomeric landscape of recombinant human QR2 expressed in Escherichia coli, preserving non-covalent interactions and enabling analysis of assembly behavior under native conditions. QR2 is predominantly observed as a dimer stabilized by multiple non-covalently […]
- Tandem mass spectrometry (MS/MS) fragments molecules into smaller pieces, generating spectra composed of m/z values and intensities that encode structural information for molecular annotation. With increasing mass spectrometry data acquisition speeds, manual annotation from MS/MS lags far behind data generation and remains a bottleneck in metabolite annotation. Current computational methods, such as molecular networking, address this challenge by organizing similar structures into families of related compounds. However, they generally provide only similarity scores, offering weak actionable insights for structural annotation. […]
- Motivation: Protein melting temperature (Tm) prediction accelerates the discovery of thermostable enzymes which are crucial for industrial biotechnology often requiring harsh reaction conditions. Experimental determination of Tm remains labour-intensive and varies across techniques, motivating the development of in silico predictors. Mass-spectrometry datasets such as Meltome Atlas now enable large-scale Tm prediction with models based on deep learning, but model generalisation across diverse experimental datasets has not been systematically tested. Results: We evaluated the generalisability of state-of-the-art deep learning approaches and […]
- J. Anal. At. Spectrom., 2026, Advance ArticleDOI: 10.1039/D5JA00495K, Paper Open Access   This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.Yuya Shimizu, Masaya Tahara, Akane Yaida, Yukiko Moriiwa, Toshihiro Takamatsu, Atsushi Shoji, Akitoshi OkinoDirect analysis of drug molecules inside solid tissue-like material using a plasma injection probe and mass spectrometry.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry
- Over the past years, proteomics has moved increasingly towards the analysis of large cohorts of biological specimens. This has been made possible by significant improvements in mass spectrometry technology, chromatographic separation methods, and improved data acquisition strategies. These technological advances now routinely enable experiments that yield vast datasets that substantially outstrip the capacity of existing proteomics data analysis approaches. Processing such large datasets requires purpose-built, quality control tools designed to organize and analyze the data while recording all processing parameters […]
- Journal of the American Chemical SocietyDOI: 10.1021/jacs.5c18659
- This new paper maybe isn't for everyone, but I'm excited to look at these scripts. There are a very small number of supported software packages out there in the world (approaching zero) that can make sense out of direct infusion of flow injection analysis quantitative data. We used to have a pile of them. Ion A is 10x higher than ion B in these two matrices, you could extract that a bunch of ways, but that's largely fallen off.I'm also excited to […]
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.6c00051
- ABSTRACT Glycopeptide enrichment is an essential prerequisite in mass spectrometry (MS)-based protein glycosylation studies, due to the low abundance of glycosylated peptides and the high complexity of biological samples, which contain substantial interference from non-glycosylated metabolites. Current enrichment strategies commonly exploit the polar interactions between glycans and hydrophilic solid-phase materials. Building on existing magnetic-core particles, we developed a series of novel magnetic materials with surfaces functionalized with dextran and amide groups. The magnetic core, composed of polyvinylpyrrolidone (PVP) and Fe3O4, […]
- Deviations from the canonical genetic code include reassignment of UAA/UAG stop codons to glutamine in divergent eukaryotes, and tRNAGln has been shown to mediate near-cognate stop codon readthrough in canonical-code organisms. However, the sequence determinants and mechanistic basis of this decoding event remain poorly understood. Using ribosome profiling, quantitative immunoblotting, and mass spectrometry in Saccharomyces cerevisiae, we demonstrate that premature stop codon readthrough efficiency is governed by both local glutamine codon context and the global glutamine codon content of the […]
- 🇮🇹 Greetings from Rome!Last week, the HUPO-PSI Spring Meeting at the CNR brought together the global community to advance open data standards for #proteomics and #metabolomics. 🧬@deNBI was thrilled to be part of the discussions! We organized a successful workshop Metabolomics & MS Data Standards fostering collaboration across the field. 🤝Standardization is key to reproducible science! 🚀#HUPOPSI #OpenData #Bioinformatics #deNBI #Metabolomics #MassSpec #FAIRdata #ScienceEvents
- Publication date: 1 October 2026Source: Talanta, Volume 308Author(s): Ilaria Belluomo, Mark J. Perkins, Patrik Spanel, George B. Hanna, Vaughan S. Langford
- For real, I've been telling people about this conversation since we recorded it a while back. Snot is way cooler than you'd guess.
- Publication date: Available online 8 May 2026Source: Analytica Chimica ActaAuthor(s): Feiyao Lu, Dan Fu, Xiuping He, Xueting Zhang, Haoshun Zhang, Tongzhu Han, Junhui Chen