- hi!! can you give me tips on how can i discuss metabolomics to undergrad students that doesnt know it at all? any analogy or anything that would help them understand. the discussion is focused on ai driven biomarker discovery. i realized that they need to have a brief discussion on metabolomics and bioinformatics too, to understand the discussion. thank you so much!! [link] [comments]
- by /u/Dependent-Alarm3338Hello! (TW: This post reeks of noob) I am an analytical chemistry grad student (first year, expected to graduate in 4), due to my background in pharmacy, I developed an interest in metabolomics and I would love to do my PhD thesis on targeted metabolomics. My advisor is an analytical chemist who doesn't have experience in metabolomics, but he is so supportive and gave me the green light to work on it if I mange to conceptualize a study. And the more I read, the more lost I feel. So, I am here to get help from the pros. Although […]
- Does anyone have experience in extracting metabolites and lipids from serum (human / bovine)? We have some issues with extracting them for Mass Spec analysis. I would be grateful if you could share working protocol if possible. I did run the lipidomics samples a couple of weeks ago. Unfortunately, I didn't really observe anything in them other than the standards that I had spiked in (which looked great, so I am confident the method works well), so I opted to not run the metabolomics as I expect the result will be the same. It seems I am still hitting this […]
- by /u/Bumblebee0000000Hi, I am sorry to bother you. In 5 months I will start a thesis in bioinformatic and metabolomics using R and machine learning. Big problem: I am interested but have no idea where to start studying. Do you know what I should read or videos I could watch to learn more about R (the program I will use), machine learning and R applied to metabolomics? I often feel overwhelmed when I have too many resources to use and I end up being desperate. Thanks in advance submitted by /u/Bumblebee0000000 [link] [comments]
- by /u/QuirkyFlower7244I'm in Metaboanalyst, processing MS peak list data (i.e. the pre-processing step). After the step of matching peaks across samples, peaks were grouped, and if there was more than one peak per group, it was replaced by their sum. My question is, how can one see which peaks (by rt and m/z) were grouped together and replaced by a sum? For example, I had ~12000 features and now it's down to about ~8000. Thank you so much for your insight! submitted by /u/QuirkyFlower7244 [link] [comments]
- by /u/HS-Lala-03Just ran a HUGE experiment with 22 conditions across 2 weeks of quenching, extraction and GC-MS runs of yeast cells. My data looks like absolute s**t. This is so demoralizing and I don't know what to do. Sorry for the post since it's not very scientific, but I'm just tired. submitted by /u/HS-Lala-03 [link] [comments]
- https://preview.redd.it/gyoq7iayyboe1.png?width=271&format=png&auto=webp&s=b4497921eaae300a97caddbb21c8f9b548830abb if you are on Discord open invitation to join our Mass Spec (Multi Omics) group. https://discord.gg/Sm6gWgpsf4 [link] [comments]
- by /u/RadiantNote922Hi there, first time LC-MS work for me! I am trying to compare the metabolite content of a plant grown in four different places. I've got the LC-MS data processed with Compound Discoverer, and at the moment i have a file with thousands of molecules and a dozen of rows, with the compound name, the area of the peak of that molecule in that sample group (average), the ratio between the groups, the ajusted p value, etc… I wanted to ask you, in general how do you analyze the data coming out from compound discovere? For example, i have got […]
- by /u/ChaochicWhat should I consider making the decision to keep only one? https://preview.redd.it/u4b1p54xmcme1.png?width=828&format=png&auto=webp&s=ff7b6ef85bd3be585a753cbfba31b3fb997cff4b submitted by /u/Chaochic [link] [comments]
- by /u/QuirkyFlower7244In the data pre-processing step in metaboanalyst, "Processing MS peak list data," metaboanalyst suggests a mass tolerance of 0.25 m/z, specifically for LC-MS peaks. However, a mass tolerance of 0.025 is pre-populated in the text box. How can I best choose the optimal mass tolerance for my LC-QToF data? I also thought m/z had no units, however I am also reading that it could equate to daltons or ppm or percentage. It is unclear what units metaboanalyst is using. I appreciate your thoughts on this! submitted by /u/QuirkyFlower7244 [link] [comments]
- by /u/Narrow-Street-4194submitted by /u/Narrow-Street-4194 [link] [comments]
- by /u/sidharth45Hello I’m going to perform some untargeted metabolomics and lipidomics of rat plasma in tumor induced model against drug treatment. The columns which I have are BEH-Hilic, Hilic-z, BEH C18 and, HSS T3 C18. For polar metabolites, I will use both of the hilics but for non polar I’m confused with the C18s. Can anyone please suggest which is the best? Due to some time constraints and instrument slot booking, I can’t spend good amount of time on optimising the columns. So please suggest me which one should I go with and can anyone pls share me some good untargted […]
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