- by /u/matthewmitchell29Recently started a new position doing proteomics work with MS. The current process involves a peptide quant assay to calculate an injection volume for each sample for the MS so that the sample load is roughly consistent. Unfortunately, this means once the values are calculated, each one is manually typed into XCalibur when creating the run setup file. With 80 samples per plate and looking to scale up the plates ran per week, this is a very time-consuming step. I'm wondering if anyone knows of an elegant solution for this as I cannot seem to simply copy and paste the […]
- by /u/SandStorm655I have two Agilent 8890/7000E GC/MSMS (EI extractor sources) in my lab that we are doing method development on. Last Thursday, I came in to find the indicator LEDs red. After restarting MassHunter it gave me a popup to power cycle the instruments. That all worked well and they came back online. I tuned one of them (all normal) and set up a sequence. It got to the third injection and I lost my chromatograms after about 4 minutes and they never came back. Now the filaments don't light up and auto tune and manual tune both say the filament […]
- by /u/aikon012Just need help setting up a lab. Would like to get refurbished equipment that is reliable. Will be likely testing protein chains that are water soluble. I reached out to a chemist and he was crazy expensive to help our team to build out a HPLC and mass spec lab. I’m looking for the best ways to acquire all the equipment and hire someone to run these tests regularly. Any suggestions would be appreciated. submitted by /u/aikon012 [link] [comments]
- by /u/massspecserviceI thought I would share this subreddit just created, this is to help support all lab equipment of every variety. We specialize in chromatography, but all questions for every flavor of lab equipment is welcome. submitted by /u/massspecservice [link] [comments]
- by /u/Silent-Possibility23Hi, Is there any way to trick Thermo into collecting data onto a network drive? Or a good reason to honor it? It feels like a restriction from the era of CAT3… but I could be wrong 🙂 submitted by /u/Silent-Possibility23 [link] [comments]
- by /u/Old_Can_825Hello Just wanted to know if anyone else have had a same problem. I have a Shimadzu LC and then also Shimadzu LCMS8050CL. This year (2026) MS sensitivity has decreased. At first I thought that okay PM is due. So tech did a PM, signal got even worse. After that I did a clean myself (interface, qarray, multipoles), signal got just alittle better, but a month later it got even worse. Tech came back, cleaned the MS again!! Including quads 2 or 3 times, still no signal/very unstable up and down. MS has been clean front to back multiple times, […]
- by /u/anasmrait12Hello, I would like to ask about the arsenic analysis in food samples using microwave digestion. After digestion, is it necessary to add KI and ascorbic acid to both the standards and the samples in order to reduce/convert arsenic species before hydride generation analysis? Or can the arsenic be analyzed directly after digestion without adding these reagents? Thank you. submitted by /u/anasmrait12 [link] [comments]
- by /u/VinyasaAndVodkaFirst time going to ASMS!!!! What should I expect 😆 submitted by /u/VinyasaAndVodka [link] [comments]
- by /u/mage1413Hi all, I have a thermo Exploris 480. I am wondering if I wanted the Biopharma option, is it a software or hardware upgrade? Or just a license? Any help is appreciated. Thank you. submitted by /u/mage1413 [link] [comments]
- by /u/Altruistic_Border381Hi everyone! I have just started working with mass spectrometry and get confused about untargeted metabolomics. How do different detection platforms affect detection accuracy and the number of identifications? Besides, what type of mass spectrometer are you currently using? Do you have any recommendations? Timstof? astral? Thank you! submitted by /u/Altruistic_Border381 [link] [comments]
- by /u/Living-Cut-89Hello everyone, I am currently working on my masters thesis and want to set up a GC-MS-IMS System for VOC fingerprinting of herbs and spices. I am using a 7890B GC System, 5977A MSD (G7040A), IonMobilitySpectrometer25 and a µFlowMgr 2-ways Splitter from Gerstel. The Splitter is without make up gas. Column is DB-35MS (30 m, 0.250 mm and 0.25 µm). In order to learn how to set up the parallel detection i read through the Document „Agilent G3181B Two-Way Splitter Kit Without Makeup Gas: Installation and Operation Guide“. I know it is „strongly recommended“ to use a Splitter with makeup […]
- by /u/mage1413Hi all, I have a 4000 Qtrap where the vacuum reading drops to almost 0.00 Torr sometimes but then shoots up to 0.5 or so according to analyst. However, if I use a multimeter and check the boards directly I find a stable vacumm reading. I already checked the filament with a multiple meter and checked the resistance and continuity and it seems fine. I also replaced the system control board and observed the same issue. I already used a new roughing pump,.hoses and clamps. Turbo seems normal on the software. Any idea? Thank you submitted by /u/mage1413 [link] [comments]
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