- by /u/nintendochemist1submitted by /u/nintendochemist1 [link] [comments]
- by /u/SenC1990Hello, I could really use some help. For my master's thesis, I am working on the analysis of Ambient-MS data, specifically peppermint/mint samples. Unfortunately, for various reasons, the samples themselves are not ideal, so I have started working on a deconvolution approach to separate overlapping signals. However, I have reached a point where my own datasets are not sufficient for proper development and validation. I am therefore looking for datasets that meet some of the following criteria: MS/MS data acquired at multiple collision energies, ideally 5 CE levels (e.g., 10, 20, 30, 40, 60% or similar). Cases where deconvolution is […]
- by /u/dynein_IRLI’m too lazy to learn ms-dial. Any real lipidomics people use mzmine only? Do you just search against LipidBlast? Or do I need to stop being lazy? submitted by /u/dynein_IRL [link] [comments]
- by /u/Extension-Cattle-671Hi everyone. I am new to this machine and I want to know how to take data in ppm units. The data i take is in raw form so i think current in Amperes? but while data taking I can change the display plot axis to ppm so the software directly shows the data in ppm meaning it can somehow do the conversion itself then it must also be able to save it right? or at least how to convert it myself- which formula to use and what factors. I will be forever grateful for any help i can get […]
- by /u/ZeffWallysubmitted by /u/ZeffWally [link] [comments]
- by /u/Upper_Assist_1791submitted by /u/Upper_Assist_1791 [link] [comments]
- by /u/Outside_Western8328I wanted to test the linearity of our qtof so did serial dilutions. Then my expectation was that the dilution times peak area would give the same "normalized area" for all dilutions in the linear range. Normalized peak area=peak area* dilution For tripple quad instrument this was very accurate but for the QTOF instrument it was not as good as I expected. Most of untargeted omics dont use cal curves so it does seem like it needs to be linear and proportional response. Anyone that has tested this and is it something wrong with my data or a common issue […]
- by /u/EarthPuzzled9675We use 3 point peak, 3 replicates, 200 sweeps/replicate. The masses selected are in the picture, and the sample introduction setting is also shown in picture. So for ~0.1s integration time per mass, doesn’t 200 sweep and 3 replicate at least take 0.1 x 5 x 200 x 3 = 300s? But after the 30s stabilization, our software takes just 2-3 seconds to complete all the acquisition. Is this normal? Also what does the total Acq time:36.240 mean in the first picture? submitted by /u/EarthPuzzled9675 [link] [comments]
- by /u/just4fun420blazeitHello everyone, We have been using an in-house QC mix (3 amino acids and their heavy isotopes) for several months. While the other two amino acids consistently perform with CVs < 10%, L-Histidine has been highly erratic for months, with a CV of 30-50%. This failure rate is causing our QC to fail during actual sample runs. For context, L-Histidine elutes at the ~1-minute mark. Because the other amino acids remain highly stable, we have ruled out sample preparation errors. Furthermore, the LC-MS system was recently PM’d, and the column has submitted by
- by /u/vaquerosupremoHello all, I am a final year PhD student in synthetic biology/molecular biology currently writing my thesis. I have genetically engineered cells to make novel therapeutics and sent samples to a company for running on LC-MS. I have the MS data and have been using MS-DIAL to search for my compounds of interest. From the MS DIAL results I have peaks which are consistent with the positive control which aren't present in my negative control – therefore I believe my cells are making my product. But I was wondering how to present this in a PhD thesis? I am not […]
- by /u/OwlScary4912I test drinking water and use a NexION 350 and a NexION 1100. The 350 works great but it’s old and the 1100 mostly work fine but the lead numbers all around drop throughout a run. It can fall sometimes -.300ug/l between calibration checks from the start of the day to the end of the day. It also starts out lower with the same calibration check when compared to the 350. We’ve tried everything and literally nothing seems to work. Any help would be be amazing would love to figure this out before the 350 completely shuts down. Doesn’t matter […]
- by /u/Training_Pangolin177The instrument was idling fine with no reading for turbo pump 1 iongauge but good reading for turbopump 2. When trying to restart the mass spec, the status on the mass spec panel keep flashing oringe and both ion gauge in orginge color. On the computer, mass spec or diagnosis is offline and no connection to the mass spec or configuraton can be made and mass hunter won't open. Doesn this mean some board on the mass spec needs to be replaced? If so whicn one is it? Thank you. submitted by /u/Training_Pangolin177 [link] [comments]
Mass spectrometry websites, social media, journal feeds, and other links and items of interest to the mass spectrometry community.
