- by /u/TonightHungry8520Hi everyone! I ran my data through SIRIUS. SIRIUS worked and exported a bunch of Excel filesā¦ but now Iām completely lost about how people actually go from these outputs to real metabolite IDs. My goal is that i only want annotated compounds that exist in HMDB (since itās biological samples and I donāt care about synthetic/random database hits). I got the files exported which are in the image, but Right now it feels like I have resultsā¦ but not something I can confidently say: āthis feature = this metaboliteā. If anyone has a practical workflow (like: open this file […]
- by /u/le_chantAs the title says, Iām looking to quantify aromatic amino acids in fermented food samples. Does anyone have any good recommendations on University cores or for-profit companies in the US for this? Iām looking for something relatively expedient (as university cores can sometimes be slow) but not crazy expensive per sample. Iāll also need sample prep. submitted by /u/le_chant [link] [comments]
- by /u/JACKHAGGETTsubmitted by /u/JACKHAGGETT [link] [comments]
- by /u/Training_Pangolin177At least the Gas 2 has problem. It is not flowing and the light for gas 2 on source board flash and then quit, but not gas 1, since both gas essentially come from the same source I suppose the gas 2 valve is bad. Is it safe to just diconnect the electric leads and gas tubes to the valves and replace the valve without vent the instrument? submitted by /u/Training_Pangolin177 [link] [comments]
- by /u/TonightHungry8520Hi all! I have experimental MS2 spectra and reference spectra that I wanna compare and present them, I just need to generate a clean mirror plot for my thesis/presentation experimental vs reference with matched peaks highlighted. Does anyone know a free, simple GUI or online tool where I can upload my sample MS2 spectra and the reference spectra and it will automatically mirror them just like the images shown. I have so many spectra and donāt want to do it manually itās ugly and time consuming. Thank you in advance! š submitted by /u/TonightHungry8520 [link] [comments]
- by /u/Training_Pangolin177For example, if the source PCB needs to be replaced, is there a way to do it while maintaining the vacuum? How about other boards? It seems there is a way to do just that on a Agilent QTOF instrument, I saw a service engineer doing that with a device inserted to a port, even though I don't know exactly what is that all about. Thanks. submitted by /u/Training_Pangolin177 [link] [comments]
- by /u/No-Region-2187Hello Experts! We are developing a method for oligosaccharide profiling in our sample and by FLR-MS. We are doing a clean-up method by C18 pierce column before labeling and after the glycan release by PNGase F. Does anyone have any experience? please let me know if this workflow should work well? Activate the C18 (Pierce) spin column with 400 ĀµL Activation Solution (50% ACN). Centrifuge according to manufacturer instructions and discard the flow-through. Equilibrate the column with 400 ĀµL Equilibration/Wash Solution (0.5% TFA in 5% ACN). Centrifuge and discard the flow-through. Prepare the released glycan sample by adding Pierce Sample […]
- by /u/CommandOwn1557I can't find any information on this. The RT in the report.tsv file in DIANN a normalized RT or unnormalized? Thanks submitted by /u/CommandOwn1557 [link] [comments]
- by /u/redditnessdudeI'm new to LC/MS, and even newer to HILIC chromatography, so I neglected to consider that the high organic starting composition in my gradient would precipitate out my aqueous mobile phase (10 mM ammonium bicarbonate). Now the ESI needle isn't spraying properly, it's just sputtering liquid everywhere, and there is also visible precipitate on the outside of the needle. Is there any recovering from this without replacing the needle? We don't have one on hand. submitted by /u/redditnessdude [link] [comments]
- by /u/MassSpecMenderSafe to say that none of the internal components were even salvageable. Except for the local scrap man that is… submitted by /u/MassSpecMender [link] [comments]
- by /u/Leonbimdoes anyone have the latest firmware for TRACE 1300 GC from Thermo? Or something relativeley new? submitted by /u/Leonbim [link] [comments]
- by /u/mage1413hi all, I recently found that my sciex MS works perfectly fine for low MW (less than 1000) compounds. however when I go over 1100 or so the machine errors out via shutting off the exhaust pump. I'm wondering if anyone has experienced this issue before? submitted by /u/mage1413 [link] [comments]
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