Murray Group ASMS 2024 Presentations

MP 377 Laser Ablation Sampling and MALDI Imaging for Tissue Lipidomics

TP 170 Deep Ultraviolet and Infrared Laser Ablation of Carbonic Anhydrase II

TP 382 Photochemical Tissue Pre-processing for Matrix-Assisted Laser Desorption Ionization Imaging of Lipids

TP 665 Deep Ultraviolet Laser Protein Footprinting

WP 379 MALDI Imaging of Lipids using Long-chain n-Alkyl Primary Ammonium and Conventional Matrix Conjugate Base Organic Salt Matrices

WP 750 Laser Ablation Sampling for Formalin Fixed Paraffin Embedded Mouse Lung Tissue Proteomics

ThP 534 Laser Ablation Electrospray of Proteins with Ion Mobility Mass Spectrometry

TP 382 Photochemical Tissue Pre-processing for Matrix-Assisted Laser Desorption Ionization Imaging of Lipids
TP 382 Photochemical Tissue Pre-processing for Matrix-Assisted Laser Desorption Ionization Imaging of Lipids
TP 665 Deep Ultraviolet Laser Protein Footprinting Kermit K. Murray*; Oluwatosin A. Ogundairo; Kadeem Hayes Louisiana State University, Baton Rouge, LA
TP 665 Deep Ultraviolet Laser Protein Footprinting

Kermit K. Murray*; Oluwatosin A. Ogundairo; Kadeem Hayes

Louisiana State University, Baton Rouge, LA
ASMS 2024: Laser Ablation Sampling for Formalin Fixed Paraffin Embedded Mouse Lung Tissue Proteomics
ASMS 2024: Laser Ablation Sampling for Formalin Fixed Paraffin Embedded Mouse Lung Tissue Proteomics
WP 750 Laser Ablation Sampling for Formalin Fixed Paraffin Embedded Mouse Lung Tissue Proteomics
WP 750 Laser Ablation Sampling for Formalin Fixed Paraffin Embedded Mouse Lung Tissue Proteomics
ThP 534 Laser Ablation Electrospray of Proteins with Ion Mobility Mass Spectrometry
ThP 534 Laser Ablation Electrospray of Proteins with Ion Mobility Mass Spectrometry
ThP 534 Laser Ablation Electrospray of Proteins with Ion Mobility Mass Spectrometry
ThP 534 Laser Ablation Electrospray of Proteins with Ion Mobility Mass Spectrometry
Deep Ultraviolet and Infrared Laser Ablation of Carbonic Anhydrase II
Deep Ultraviolet and Infrared Laser Ablation of Carbonic Anhydrase II
ASMS 2024
ASMS 2024
WP 379 MALDI Imaging of Lipids using Long-chain n-Alkyl Primary Ammonium and Conventional Matrix Conjugate Base Organic Salt Matrices
WP 379 MALDI Imaging of Lipids using Long-chain n-Alkyl Primary Ammonium and Conventional Matrix Conjugate Base Organic Salt Matrices
TP-665-DUV-Laser-Protein-Footprinting
TP-665-DUV-Laser-Protein-Footprinting
TP-665-Deep-Ultraviolet-Laser-Protein-Footprinting
TP 382 Photochemical Tissue Pre-processing for Matrix-Assisted Laser Desorption Ionization Imaging of Lipids
TP 382 Photochemical Tissue Pre-processing for Matrix-Assisted Laser Desorption Ionization Imaging of Lipids
MALDI Imaging of Lipids using Long-chain n-Alkyl Primary Ammonium and Conventional Matrix Conjugate Base Organic Salt Matrices.pdf
MALDI Imaging of Lipids using Long-chain n-Alkyl Primary Ammonium and Conventional Matrix Conjugate Base Organic Salt Matrices.pdf
MP 377 Laser Ablation Sampling and MALDI Imaging for Tissue Lipidomics Kadeem O. Hayes and Kermit K. Murray
MP 377 Laser Ablation Sampling and MALDI Imaging for Tissue Lipidomics

Kadeem O. Hayes and Kermit K. Murray

ASMS 2024: Deep Ultraviolet and Infrared Laser Ablation of Carbonic Anhydrase II

Deep Ultraviolet and Infrared Laser Ablation of Carbonic Anhydrase II

Neda Feizi1; Kadeem O Hayes2; Blessing Chisom Egbejiogu2; Kelcey B. Hines2; Kermit K. Murray2; Touradj Solouki1
1Baylor University, Waco, TX; 2Louisiana State University, Baton Rouge, LA

Introduction
Integrating ion mobility-mass spectrometry (IM-MS) with laser ablation (LA) holds great appeal for acquiring spatially resolved details on protein conformations. Previously, we showed that protein conformation remained intact after LA. However, the potential for laser-induced unfolded proteins to refold back into native-like structures in the capture-solvent remains unresolved. In contrast to typical proteins, denatured bovine carbonic anhydrase II (CA) does not revert to its original conformation in native capture-solvent; instead, it aggregates. This characteristic makes CA particularly well-suited for investigating conformational changes in native surface mass spectrometry (NSMS). Here, we employ NSMS to explore protein unfolding and refolding events, enabling us to examine conformational stabilities during infrared (IR) and deep UV (DUV) laser ablation experiments.
Methods
All solvents and chemicals were purchased from commercial sources and used without further purification. CA stock solution was prepared at 750 µM concentration in native solution of 200 mM ammonium acetate (NH4OAc) adjusted to a pH of 7.3 ± 0.1. Four microliters of the stock solution was deposited on the quartz microscope slide and air-dried. Either a 193 nm ArF excimer laser or 2.94 µm OPO laser was used at a repetition rate of 20 Hz to ablate CA from the surface. All laser ablation experiments were performed in transmission geometry with capturing in 20 µL of native solution. Native IM-MS and broadband collision-induced unfolding (CIU) data were acquired using a Waters Synapt G2-S instrument (Milford, MA).
Preliminary Data
Native mass spectrometry was utilized to probe the charge states and CIU pathways of CA. After deposition and drying on a glass microscopic slide, CA was either washed with native solution or ablated with IR or DUV lasers into the native solution. To minimize variations in mass spectra, including observed charge states and peak shapes, all ESI emitters were inspected with SEM before acquiring mass spectra and only emitters with tip diameters between 600 ± 100 nm were utilized. Mass spectra from triplicate washed and laser-ablated CA showed analogous patterns and charge state distributions (+8 to +10). Moreover, the statistical t-test indicated that the average charge states and peak areas of the washed and laser-ablated CA were not significantly different. The broadband protein ion unfolding experiments were performed without quadrupole ion isolation of specific charge states. Ion collisional activation was achieved in the pre-IM trap cell by establishing a potential difference from 10 to 100 V in 5 V increments. CIU plots showed that the number of unfolding intermediates and the onset voltages for each unfolding transition of washed and laser-ablated CA were similar. The findings from complementary MALDI and bottom-up analyses of native, refolded, and aggregated CA corroborate these observations. The presented work suggests that the native-like conformation of CA remains unchanged after DUV and IR laser ablation; therefore, laser ablation can be used for NSMS.
Novel Aspect
Carbonic anhydrase II serves as a unique probe, enabling the investigation of structural stability during IR and DUV laser ablation.

ASMS 2024 Presentations

LSU Professor Kermit Murray Selected Senior Member of National Academy of Inventors

Roy Paul Daniels Professor of Chemistry Kermit K. Murray has been selected a senior member of the National Academy of Inventors.

This year’s class comprises 124 accomplished academic inventors representing 60 research universities, governmental entities and nonprofit institutes worldwide.

The Senior Member program is an exclusive award created to showcase the innovative ecosystems at NAI member institutions, like LSU, which provide the supportive environment to foster novel discoveries. Senior members are chosen from active faculty, scientists, and administrators with success in patents, licensing, and commercialization who have produced technologies that have the potential for a real impact on the welfare of society.

“It is a great honor to be selected as a senior member of the National Academy of Inventors,” Murray said. “I am thankful to all the wonderful students, colleagues, and collaborators who share my passion for lasers and mass spectrometry and helped make this possible.”

“This year’s class of Senior Members is truly a testament to the outstanding innovation happening at NAI Member Institutions and what happens when the academic space encourages and celebrates invention and commercialization,” said Paul R. Sanberg, President of NAI.

Murray is a leader in the development of instruments for mass spectrometry. He has broken new ground in the sampling, measurement, and imaging of biological samples using lasers. By expanding the capabilities of mass spectrometry itself, Murray has enabled the application of this powerful technique to problems that were previously inaccessible, such as microsampling at cellular precision. And through his dedicated mentorship of students here at LSU, by routinely involving them in the creation of new technologies, he has instilled his enthusiasm for innovation in the next generation of scientists.

He began his LSU career in 2001. His many honors include LSUs Rainmaker Senior Scholar Award and the American Society for Mass Spectrometry Research Award.

Murray holds four U.S. patents in the field of mass spectrometry and is the founder of a startup company, Laser Bioanalytics. He was also instrumental in the commercialization of laser systems now in production by Opotek.

He has published over 140 scholarly articles and serves on the editorial board of Analytica Chim Acta and as editor of a mass spectrometry terminology website. Murray is a Fellow of the International Union of Pure and Applied Chemistry (IUPAC) and member of the American Chemical Society, American Society for Mass Spectrometry, International Mass Spectrometry Society, and IUPAC.

Murray and the other senior members will be celebrated at NAI’s annual conference June 16-18 in Raleigh, N.C.

About LSU’s Office of Innovation & Technology Commercialization

LSU’s Office of Innovation & Technology Commercialization (ITC) protects and commercializes LSU’s intellectual property. The office focuses on transferring early-stage inventions and works into the marketplace for the greater benefit of society. ITC also handles federal invention reporting, which allows LSU to receive hundreds of millions of dollars each year in federally funded research, and processes confidentiality agreements, material transfer agreements, and other agreements related to intellectual property.

About LSU’s Office of Innovation & Ecosystem Development

LSU Innovation unites the university’s innovation and commercialization resources under one office, maximizing LSU’s impact on the intellectual, economic, and social development of Louisiana and beyond. LSU Innovation is focused on establishing, developing, and growing technology-based startup companies. LSU Innovation oversees LSU Innovation Park, a 200-acre business incubator that fosters early-stage tech companies, and the Office of Innovation & Technology Commercialization, which streamlines the process of evaluating, protecting, and licensing intellectual property created by LSU researchers. LSU Innovation serves as the host organization for the Louisiana Small Business Development Center (SBDC) Network which oversees all SBDC services across the state as well as the LSU SBDC, which provides free consulting services to small businesses across the state. LSU Innovation helps Louisiana technology companies apply for seed funding through the federal Small Business Innovation Research and Small Business Technology Transfer grant programs. LSU Innovation educates faculty, students, and the community on entrepreneurial principles through the National Science Foundation’s Innovation Corps (I-Corps) program which trains innovators to consider the market opportunities for pressing scientific questions, leading to increased funding state and federal grant programs as well as potential industry partners and licensees.

Assistant Professor Faculty Position in Analytical Chemistry at LSU

Apply Online

The Department of Chemistry in the LSU College of Science seeks a tenure-track faculty member in analytical chemistry for an August 2024 start date. Candidates are required to have strong experience in analytical chemistry, broadly defined. Preference will be given to those having research interests aligned with the LSU Scholarship First Agenda and focusing on chemical analysis methods for agriculture, biomedicine, coastal environment, defense, and energy. The successful candidate will join a dynamic research environment with instrumentation resources including NMR spectroscopy, mass spectrometry, x-ray crystallography, optical and electron microscopy, and polymer analysis and will have multiple opportunities to collaborate within the Chemistry Department and across the Louisiana State University System. A high priority for hiring is an experimentalist using NMR, especially the 700 MHz Bruker Avance Neo. Inclusiveness and diversity are critical to the success of the Department, the College of Science, and the University. The selected candidate will be expected to foster an environment that is supportive and welcoming of all groups.