Infrared laser ablation sampling coupled with data independent high resolution UPLC-IM-MS/MS for tissue analysis

M.E. Pettit, F. Donnarumma, K.K. Murray, T. Solouki, Infrared laser ablation sampling coupled with data independent high resolution UPLC-IM-MS/MS for tissue analysis, Anal. Chim. Acta. 1034 (2018) 102–109. doi:10.1016/j.aca.2018.06.066.

Infrared laser ablation microsampling was used with data-dependent acquisition (DDA) and ion mobility-enhanced data-independent acquisition (HDMS^E) for mass spectrometry based bottom-up proteomics analysis of rat brain tissue. Results from HDMS^E and DDA analyses of the 12 laser ablation sampled tissue sections showed that HDMS^E consistently identified approximately seven times more peptides and four times more proteins than DDA. To evaluate the impact of ultra-performance liquid chromatography (UPLC) peak congestion on HDMS^E and DDA analysis, whole tissue digests from rat brain were analyzed at six different UPLC separation times. Analogous to results from laser ablated samples, HDMS^E analyses of whole tissue digests yielded about four times more proteins identified than DDA for all six UPLC separation times.