- by /u/alycatdabraHi- I'm getting acquainted with an Agilent Ultivo QQQ LCMS system. I'm working with a metal complex compound that ionizes great on QExactive and when I do direct injections on the Ultivo. However, when using the LC in conjugation, the ionization completely changes. I no longer see higher masses and only see fragments. I'm guessing that it's something about the increased flow suppressing ionization (0.25 mL/minute). All other parameters are kept the same. I've experimented with sheath gas flow, gas temperature, and ionization energy but nothing is giving me the parent peak any more. I've tried using injections without the […]
- by /u/Slinkyfest2005Hiya folks, I'm relatively new to the whole GCMS thing and as is tradition dictates there isn't really anyone more familiar with the instrument than I am, sad to say. It's a Thermo 1310 with ISQ 7000 MS. Having inherited the machine and knowing the last people to use it, I think they cranked the spring loaded transfer line nut closed with a wrench to try and ensure a seal. Now the damn thing is cutting columns in half when I try to put them in, and it won't accept a 0.53ID column at all. Any suggestions for fixing this […]
- by /u/Expensive-Painter-18submitted by /u/Expensive-Painter-18 [link] [comments]
- by /u/Replica72Hi! I work for a company in LA that has a clinical lab and wants to also open an analytical lab with mass spec for both peptides (quality control) and hormones (clinical testing). Im looking for an expert in the LA California area who can help us with method development. Anyone interested or know someone who may be? submitted by /u/Replica72 [link] [comments]
- by /u/Workflow_ScientistI’m trying to see whether this is actually useful for real-world issues like carryover, signal instability, peak shape problems, retention time shifts, contamination suspicion, and similar headaches. Instead of going through vendor manuals, app notes, and PDFs, you ask the troubleshooting question directly and get a scientific and deep answer grounded in the source material. I’d really appreciate feedback from people who actually deal with this stuff. submitted by /u/Workflow_Scientist [link] [comments]
- by /u/Ok_Bake_4761Hello, I am working with an Agilent 5977B with Helium as a carrier. I got a message from our gas supplier that due to the blockage of the strait of Hormuz, we won't get Helium any time soon. We bought a 50L bottle 15 years ago for 150€, and I believe it's going to be 1000€ per 50L bottle in the near future for us. It's a weird idea and I bet someone thought about this already, but why is it not yet economically suitable to "recycle" the used He carriergas by capturing and cleaning it. Is something even possible? […]
- by /u/Technical-Oil1890Bonjour à tous ! Je souhaite analyser mes données LS-MS avec MZMINE 4, mais je ne sais pas comment l'utiliser. Je souhaite visualiser les spectres MS/MS de deux ions différents, mais je ne trouve pas cette fonctionnalité dans le logiciel. Je peux cependant visualiser les spectres MS1 et MS2. Pouvez-vous m'aider ? Pour information, mes fichiers d'analyse sont au format .raw. submitted by /u/Technical-Oil1890 [link] [comments]
- by /u/One_Secretary_6458I had some issues with the source casings. After fixing that a reboot was needed and this error message appeared. Does someone know what to do? submitted by /u/One_Secretary_6458 [link] [comments]
- by /u/Optimal_Reach_12I recently performed the annual pm needed for our genius xe35 nitrogen generator. it all seemed to go fairly well, replace a few filters and seals in the pumps but now we sometimes get a "low nitrogen tank pressure" warning. when it gives this warning the tank is still reading 100psi that the generator is set to. Does anyone know why this might be (it might be that I didn't quite do the pm correctly but I believe I did)? submitted by /u/Optimal_Reach_12 [link] [comments]
- by /u/Left-Distribution868Help! My raw data file in google drive have been converted to .dat file. I downloaded the zip and tried to unzip it using various extracting softwares, it still remains as .dat file and won't go back to it's original file type. Can I ask if you guys have the same experience and what did you do? Did you resolve this? submitted by /u/Left-Distribution868 [link] [comments]
- by /u/Ragnorus_Hello everyone, For several months now, I’ve been trying to get a TSQ Quantum Ultra mass spectrometer up and running again—one that was rescued from the dumpster (even though it was fully functional)… I had gathered everything needed to get it working, including the PC with all the software. It seemed like a great opportunity to get started with mass spectrometry. Unfortunately, following a power surge in the building, the unprotected PC was fried… I have since been able to restore the PC to its original state (Windows 7) and reinstall the various software packages using the thermo.flexnetoperations website, and […]
- by /u/mage1413Hi all, I have a Agilent 6230B ToF with a MMI source. During a scan with a tuning solution Im finding that APCI shows good peaks but when I switch to ESI it's a complete mess. I was wondering if anyone else has faced a similar issue. thank you submitted by /u/mage1413 [link] [comments]
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