- by /u/obnoxious_brainHi everyone, I’m trying to use the post-identification feature in MS-DIAL, but I’m stuck. I copied the text file format exactly as shown in the official documentation/website (with the column headers and everything). But when I load it: • MS-DIAL either immediately crashes, or • It just refuses to work and doesn’t process anything. I’ve tried retyping the file, checking tabs/spaces, and saving it as plain .txt (UTF-8), but still no luck. Could anyone share a working post-identification text file example or tell me the most common pitfalls (like encoding, column order, missing fields, etc.)? I feel like I’m missing […]
- by /u/NegativeLeader1014Has anyone got any real pricing on this instrument that they can share? submitted by /u/NegativeLeader1014 [link] [comments]
- by /u/SquirrelKitchen8188Hi everyone, I’m using a Shimadzu GC-MS TQ8030 and ran into a problem. The capillary column broke right at the transfer lin,e and a small piece is stuck inside. I already turned off the carrier gas and let the system cool down, but I’m not sure what the safest way is to remove the broken piece, clean the ferrule or transfer line area if there’s graphite debris, and then reinstall the column without damaging anything. If anyone can walk me through the steps or share tips from experience with this instrument, I’d really appreciate it. Note: I also read that […]
- by /u/Forward_Industry_646Hello all, I work on quite an outdated instrument, the Nexion 300x from PerkinElmer. Recently, I’ve been fighting a never ending battle to keep the plasma on. I’m wondering if anyone has worked on this instrument or has had any similar issues with their instruments. Here’s the rundown: The plasma will go out and give various error codes such as nebulizer gas flow RB errors or RF power errors. The plasma outage is coupled with a loud screeching sound, almost like a record scratch. It doesnt seem like the problem is the RF power supply, as we have had that […]
- by /u/EchoNomad777A few months ago, our lab conducted a study using HS/GC-MS. The instrument was last calibrated about 3 months before the study. Unfortunately, we didn't have hydrocarbon (n-alkane) standards at the time, so we proceeded without them. Around 10 samples were analyzed. Now we’ve run into an issue: the calculated retention index (RI) values differ significantly from literature values, even though we’re confident in the compound identifications (we cross-verified the spectra with multiple references). The problem is, for any journal to accept the work, these RI values need to be closer to published values—ideally within ±10–20 units. Re-running the samples […]
- by /u/S0IIUXHey everyone, I’ve been trying to figure out this issue. I recently switched columns on my GCMS and my foreline pressure is 74-75 mTorr when this instrument typically gets around 45-50mTorr. I’ve checked everywhere for potential leaks and reseated the column at the transfer line. The air and water tune looks great, and the MS passes smart tune (even passing a leak check – 5% of reference). Any ideas on how to get this pressure down? Thanks! submitted by /u/S0IIUX [link] [comments]
- by /u/Professional_Math964Sciex 4500 vacuum is not reaching operating pressure. Turbo pump was making a loud noise and upon troubleshooting it seems to have seized. The turbo pump was replaced with a backup we had that is functional but when the instrument is turned on it doesn't reach operating pressure, the pump light just keeps blinking. The turbo pump controller (TD400) does turn on and it does turn on the turbo pump as I can hear it going but it doesn't reach pressure and the controller's red error light turns on and the turbo pump stops. It kicks on again after a […]
- by /u/Powerful_Woodpecker4Hey everyone! I’m currently running Chromeleon 7.3.2 and I have two SSL inlets installed on my GC. I had both of them installed properly, everything was fine, but one day I looked and my SVOC inlet was gone and the Purge inlet got duplicated in the instruments tab on Chromeleon. I checked the local instrument controller and everything looks fine, I see both inlets and it’s not throwing any errors. However I can’t see the second inlet in Chromeleon anymore, thus I can’t confirm if gas is flowing, temperatures are good, etc. Anyone got any suggestions on how to get […]
- by /u/RolandofGilead1000Hello All, I am hoping to get some feedback about some possible MS purchases. We are a pharma lab doing mostly biologics (peptide map, subunit, intact) that also have some small molecule applications. I am fairly certain I have decided on a Exploris 240 with biopharma extended mass range. I am putting a 2DLC on the front to do online trypsin digest to setup a more automated peptide map workflow, but also can do 2DLC intact if needed on this system. The second MS is where I am struggling to decide. A TOF is my default for larger biomolecules but […]
- by /u/AstrojonnieSo, my lab is looking at getting an ICP-MS to replace our ICP-OES. We have gotten a few quotes and the price values are so greatly different, and I am not sure why… We started with PerkinElmer, and that looked like a good fit for us, with a price that fit our budget…until PE started playing games on the Terms and Conditions end of the contract generation side. Next up was Aligent Technologies (Aligent 8900), which we REALLY liked! then came the price tag and the sticker shock was difficult to swallow. We are still looking at the Aligent, going […]
- by /u/Outside_Western8328Hello, I have annoying leak on primary pump seems to be in the interface where seal wash is. Where high pressure seal and wash seal are. I replaced the high pressure seal and seal wash seal and piston but this did not fix the problem as I was expecting. The assembly looked good no visable scratches. I used seal installation tool and it looked fine. The leak is small and starts over 700bars. Is agilent 4000 grit paper ok for cleaning pistons? Anyone have a guess what the cause could be? submitted by /u/Outside_Western8328 [link] [comments]
- by /u/oops_no_nameHello, I'm a PhD student in MS imaging and after seeing the cost of new ITO glass slides, my professor asked me to try and reuse old ones. Has anyone ever washed ITO slides and/or does anyone know how this could be achieved? I already tried sonicating in water, ethanol and acetonitrile but there is still some tissue samples on the slides. submitted by /u/oops_no_name [link] [comments]
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