- by /u/Altruistic_Spring104Hello hive mind, Our lab has an Agilent LCMS 1260 infinity II hplc + 6135B lcms stack and recently a leaving lab left us a fraction collector from their HPLC stack that we want to add into the stack. The only issues were running into is the fact that our license is locked at 5 modules and refuses to talk to the FC. Anybody know a way around this? submitted by /u/Altruistic_Spring104 [link] [comments]
- by /u/nintendochemist1Hello, We have a Perkin Elmer NexION2000B running Syngistix 3.4 that is currently down and awaiting a visit from our engineer. The issue I've observed has been intermittent at best. A different engineer, not our regular one, tried to replicate it and left when they couldn't. The issue occurs when we attempt to tune the system with SmartTune. Our torch alignment shows a readback of 0 for In 115 and stops after three iterations, but the results window to the right will show "Passed" with some random number. This happens every now and then, and we can't figure out why. […]
- by /u/DamnYouMetalIonsPut me out of my misery, please I'm running samples of RNA in our OrbiTrap via direct injection through the ESI source, and I'm getting sodium adducts that are half the intensity of the damn peak itself. Group wisdom is to spray 50% methanol with 1% acetic acid through the source and then wait until the next day, but I've had limited success with this, and I'm hoping to find some other approaches submitted by /u/DamnYouMetalIons [link] [comments]
- by /u/nitsujdleifHi all, I'm an honours student working on an LCMS proteomics project using a Bruker Compact QTOF. The project is focussing on the optimization of HPLC and MS parameters for complex proteome analysis (eventually some modified glioblastoma cells). We want to start tinkering with the MS parameters, but I've had a difficult time finding publications that use this instrument for the analysis of complex protein samples. Does anyone know of any relevant publications that could provide a sound starting point in the way of MS params? Or does anyone have personal experience working with this instrument for proteomics analysis? I […]
- by /u/kiaruchemI was searching for a book regarding soft fragmentation mechanism (ESI LC-MS) I already have McLafferty's "Interpretation of mass spectra" and I like it but it's more focused on EI hard fragmentation, so I was wondering if there's a book (or any kind of resource) that explains soft fragmentation (and I would prefer something that goes into details, maybe with exercises too) EDIT: thank you for all the suggestions. I'll take a look if I can find the books you have suggested submitted by /u/kiaruchem [link] [comments]
- by /u/Sweet_Ferret_840I am looking at purchasing a Thermo Fisher Scientific X Series 2 ICP-MS Spectrometer. It is a model from around 2005. The seller got it surplus from a university, it is in working condition, but he does not a computer with software or discs included. I was wondering if there was any way to get the software online(eBay, etc.) or maybe an emulator of some kind. I believe the software is the PlasmaLab version. submitted by /u/Sweet_Ferret_840 [link] [comments]
- by /u/ExtremeSplatHello all! I recently came across a nearly free nEXT400IID (B832-00-816) turbomolecular pump that I was able to pick up. The problem that I am having now is determining how to adapt this specific pump to a standard ISO/DN flange. This pump appears to be purpose built for Thermo Fisher mass spectrometers (see the link below for what I mean) so has a specifically designed upper flange for (conceivably) mounting into their machines. The flange is meant to "allow evacuation from three vacuum chambers" but the specific plumbing of these ports appears that anything other than the primary turbo inlet […]
- by /u/Fine-Pie-4536I’m not very familiar with Proteome Discoverer, but it’s the only program we have that can perform database searches and matching. I recently developed an immunoaffinity assay and would like to run a broader proteomics analysis on my data. Normally, I focus on targeted analyses and don’t need a search engine. However, in this experiment, I have both control samples and assay samples, each containing serum plus my analyte. My assay removes most of the proteins, making it more sensitive, whereas the control is just extracted (intact) serum. I don’t perform any digestion, because it’s not required for my analyte. […]
- by /u/ChronicEntropicsubmitted by /u/ChronicEntropic [link] [comments]
- by /u/nintendochemist1Hello! I have been working on calibrating our LTQ after getting it up and running, but I'm encountering an issue that I can't figure out. You have all been a great help so far, so I'm hoping someone can point me in the right direction. My parameters are in the top right of the first picture. When infusing the cal mix standard, I can clearly see the 195, 524 and Ultramark peaks: https://preview.redd.it/3t3uqqel0gde1.png?width=1920&format=png&auto=webp&s=761696d2ae94ddee2271237460fe2b218f2691ae Knowing that the mass cal procedure uses SIM, I checked the ions there and saw them there as well (only showing caffeine to keep it from being […]
- by /u/DrymoglossumAn Open invitation to join mass spectrometry omics discord group mass spectrometry omics discord group submitted by /u/Drymoglossum [link] [comments]
- by /u/Hour_Future987Does anyone know of any remote chromatography review type of jobs for a Chemist? Part time or full time? submitted by /u/Hour_Future987 [link] [comments]
Mass spectrometry websites, social media, journal feeds, and other links and items of interest to the mass spectrometry community.
