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Molecular glue degraders (MGDs) are proximity-inducing molecules that promote the destruction of disease-causing proteins by stabilizing novel interfaces between E3 ubiquitin ligases and target proteins. The rational design of MGDs remains exceptionally challenging, historically relying on serendipitous discoveries. Here, we deployed a high-throughput, mass spectrometry (MS)-based screen evaluating thousands of cereblon (CRBN)-directed compounds to expedite the identification of novel neosubstrates. […]

Analyst, 2026, Accepted ManuscriptDOI: 10.1039/D6AN00421K, PaperYang Liu, Zhengyang Guo, Siyang Qin, Xuxiao Zhao, Xiaopei Wu, Jing Jing, Jianghai LuIn this study, the metabolic profiles of MK-3984, a selective androgen receptor modulator (SARM) targeting muscle and bone tissues, were comprehensively investigated for the first time using Quadrupole Orbitrap LC-MS/MS…The content of this RSS Feed (c) The Royal Society of Chemistry

Chemistry – A European Journal, Volume 32, Issue 23, 16 June 2026.

Chemistry – A European Journal, EarlyView.

Inositol phosphates (InsPs) and inositol pyrophosphates (PP-InsPs) are key regulators of cellular signaling and metabolism, yet developmental changes in the InsP/PP-InsP network in eukaryotic systems remain poorly understood. Here, we combine isomer-resolved capillary electrophoresis-mass spectrometry (CE-MS) with genetic perturbations to characterize InsP metabolism during the Drosophila melanogaster development. Our analyses reveal extensive developmental reprogramming of the InsP pathway, with early […]

Publication date: 1 December 2026Source: Talanta, Volume 310Author(s): Eliézer de Oliveira, Carla Beatriz Grespan Bottoli

Scaling covalent ligand discovery through dynamic combinatorial library-versus-proteome screening

Journal of Mass Spectrometry, Volume 61, Issue 7, July 2026.

Capelin (Mallotus villosus), a key forage fish in the Northwest Atlantic, links zooplankton to predators including commercial fishes, seabirds, and marine mammals, yet its life-cycle movements in the Gulf of St. Lawrence (GSL) remain poorly understood. Between 2022 and 2024, otoliths from 927 individuals collected during and after spawning were analysed by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS). […]

ABSTRACT Rationale Nitrilases are widely used as biocatalysts in pharmaceutical manufacturing because of their high stereoselectivity and mild reaction conditions. However, residual nitrilase in final drug products may compromise product quality and safety, highlighting the need for sensitive and reliable analytical methods for its monitoring and control. Methods In this study, a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed […]

ABSTRACT Rationale Ametryn poses significant risks to aquatic ecosystems, but conventional MRM methods suffer from matrix interference and limited sensitivity. To overcome these limitations, we established a more selective and sensitive UHPLC-MS3 approach that effectively suppresses background noise and enhances signal-to-noise ratio, enabling reliable quantification of ametryn in zebrafish tissues. Methods A novel UHPLC-MS3 method with electrospray ionization was developed. […]

Constructing atomically coupled W–O–Ru interfacial units on RuO2 triggers a dynamic strain evolution during the oxygen evolution reaction. An initial tensile strain secures structural stability, while a self-adjusting transition to interfacial compression optimizes catalytic activity. This dynamic strain engineering effectively decouples the long-standing activity-stability trade-off, enabling highly efficient and durable acidic water oxidation. ABSTRACT Developing highly active and durable acidic […]

Managing plastic pollution is challenging because current physical and chemical recycling methods are inefficient and environmentally intensive. Biological recycling approaches have been framed as sustainable alternatives but are challenging to optimize due to a lack of process analytical technologies that provide real-time data on microbial plastic metabolism. In this study, we used Piscinibacter sakaiensis 201-F6, a model bacterium with a […]

Liquid chromatography-mass spectrometry (LC-MS) untargeted metabolomics detects thousands of metabolic features, but converting these chemical signals into metabolite set-level biological knowledge remains challenging. This is because most features lack unambiguous metabolite identities. Conventional metabolite set enrichment analysis (MSEA) generally requires identified metabolites and metabolite-level ranked inputs, leaving much of the untargeted feature space unused. Here, we present FeatureMSEA, a feature […]

Melanocortin 2 receptor (MC2R) is a G protein-coupled receptor (GPCR) for adrenocorticotropic hormone (ACTH), and its trafficking and signalling are associated with the melanocortin receptor accessory protein (MRAP). Mutations in either MC2R or MRAP disrupt this signalling and cause familial glucocorticoid deficiency. Here, we combine native mass spectrometry (MS) and hydrogen deuterium exchange mass spectrometry (HDX MS) to uncover how […]

Post-translational modifications usually occur under enzymatic control as a mechanism for regulation of protein activity or localization, but can also occur spontaneously during protein aging and degradation. In contrast, there are few examples of spontaneous post-translational modifications with a significant role in the stability or biochemical function of a bacterial protein. Here we show that a spontaneous post-translational modification is […]

ABSTRACT Rationale Schisandrol B (Sol B) is a major bioactive lignan compound extracted from the dried fruits of Schisandra chinensis (Turcz.) Baill. with comprehensive pharmacological activities. The study aims to investigate the metabolic stability and metabolic characteristics of Sol B in rat liver microsomes (RLMs) and human liver microsomes (HLMs) and further clarify the metabolic differences among different species. Method […]

ABSTRACT Rationale Teriparatide (TPT) is a synthetic peptide primarily used in the clinical treatment of osteoporosis, with its reference materials available from both synthetic and recombinant DNA origin used for generic drug preparations. Considering the potential toxicological effects of structurally related impurities, systematic characterisation of the impurity profiles of TPT reference materials arising from different synthetic sources is essential for […]

High-resolution mass spectrometry is routinely used for the analysis of complex samples in pharmaceutical, environmental, and omics related studies. Such applications require instrumentation to be capable of combining sub-ppm mass accuracy, high resolving power, rapid full m/z range acquisition, and a wide dynamic range. Achieving these requirements simultaneously places constraints on analyzer design and performance. Multi-reflecting time-of-flight (MRT) based analyzers […]

The roles of reactive oxygen species (ROS) as signaling molecules and inhibitors of phytoplankton growth are well documented. While phytoplankton physiological mechanisms for ROS detoxification are well characterized, the role of heterotrophic bacterial partners in ROS alleviation and outcomes for these bacteria remain poorly understood. Here, we examined how extracellular hydrogen peroxide (H2O2) shapes nutrient exchange between the diatom Phaeodactylum […]