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Publication date: Available online 12 July 2026Source: Journal of ProteomicsAuthor(s): S. Wislet, C. Le Goff, Massonet Ph, S. Hody, J.L. Croisier, V. D’orio, J.F. Kaux, G. Mazzucchelli, R. Cloots

Publication date: Available online 11 July 2026Source: Journal of ProteomicsAuthor(s): Dirk Winkelhardt, Julia P. Schessner, Sven Berres, Alireza Nameni, Armin Soleymaniniya, Ayla Schröder, Caroline Jachmann, Douwe Schulte, Georg Wallmann, Henry Webel, Johannes Rainer, Katerina Nastou, Laurent Gatto, Magnus Palmblad, Maike Weber, Omar Shouman, Pavel Sinitcyn, Ralf Gabriels, Robbe Devreese, Tim Van Den Bossche

With the rapid development of mass spectrometry-based proteomics, the volume of phosphoproteomic data has increased substantially. However, accurate localization of phosphorylation sites and standardized statistical validation remain critical analytical bottlenecks. To address the lack of standardized cross-algorithm evaluation, we introduce onsite, a unified and open-source Python framework. onsite integrates an alanine-decoy strategy to estimate the false localization rate (FLR) across […]

Heterozygous de novo missense mutations in the EEF1A2 gene encoding translation elongation factor eEF1A2 result in neurodevelopmental disorders, typically characterised by early onset epilepsy and intellectual disability (ID). The E122K mutation is the most commonly reported missense mutation and is amongst the more severe in terms of epilepsy and ID. Here we made use of a recently developed mouse model […]

ABSTRACT Loureirin D is a bioactive dihydrochalcone isolated from Dracaena resin with very high therapeutic value. A sensitive and high-throughput ultra-performance liquid chromatography–mass spectrometry (UPLC–MS/MS) method was developed for the quantitative analysis of loureirin D in rat plasma with only 50 μL of sample per assay. Sample extraction was protein precipitation using acetonitrile, and isoscoparin was used as internal standard (IS). […]

ABSTRACT SHR6508, a novel calcimimetic agent, is currently being developed for treating secondary hyperparathyroidism in chronic kidney disease (CKD) patients requiring maintenance hemodialysis. For the purpose of quantifying SHR6508 in human plasma, a sensitive and reliable LC–MS/MS method was first developed and successfully validated. Since SHR6508 is a disulfide bond-containing peptide, ascorbic acid was supplemented into blood samples during collection […]

Hot chemistry in cold ices reveals the previously elusive sulfinothioic acid molecule, HS(O)SH, prepared through nonequilibrium chemistry in low-temperature ices and identified with isomer selective photoionization reflectron time-of-flight mass spectrometry combined with isotopic labeling. ABSTRACT Low-oxidation-state sulfur oxyacids are key yet experimentally elusive intermediates in interstellar sulfur chemistry; even the simplest member, sulfinothioic acid (HS(O)SH), has neither been detected in […]

Publication date: October 2026Source: TrAC Trends in Analytical Chemistry, Volume 203Author(s): Wen Li, Bin Hu

This study investigated site-specific N-glycosylation alterations in thyroglobulin from patients with papillary thyroid carcinoma using nano-LC–MS/MS. Advanced bioinformatics and machine learning identified these unique glycan signatures, establishing the Asn1365-HexNAc(3)Hex(6) glycoform as a highly predictive diagnostic biomarker (AUC = 0.83). ABSTRACT Thyroglobulin (Tg) is a clinically established biomarker for thyroid cancer; however, its diagnostic specificity remains limited due to confounding benign conditions. This […]

Natural compound phosphonothrixin's herbicidal activity resides exclusively in its (S)-enantiomer, yet existing enantioselective syntheses are cumbersome, and direct chiral LC separation is hampered by the compound's high polarity, lack of aromatic groups, and metal chelation–induced peak tailing. By employing a zwitterionic sulfohexyl quinidine carbamate, stationary phase reliable analytical and preparative chiral separations were achieved, enantiomers isolated, and absolute configurations assigned […]

Journal of Mass Spectrometry, Volume 61, Issue 8, August 2026.

This study established the first comprehensive pharmacokinetic profile of Mailuoning Oral Liquid by tracking 39 compounds in rats, identifying 15 key systemic markers via UHPLC-HRMS and validated UHPLC-MS/MS assays. Dose-dependent absorption was observed, providing an evidence base for dose refinement and clinical application. ABSTRACT Mailuoning oral liquid (MLN) is a state-approved multi-herb formula that has been prescribed for 25 years […]

ABSTRACT Solanum americanum Mill. and S. nigrum Linn. are traditional Solanaceae-derived herbal medicines. Their sprouts and mature fruits are utilized as both vegetables and medicinal herbs. Although S. americanum Mill. is classified as a primary variety of S. nigrum Linn., significant pharmacological variations and incompletely characterized phytochemical profiles differentiate their applications. In this study, UHPLC/LTQ-Orbitrap mass spectrometry (HRMS) (Thermo Fisher […]

ABSTRACT Pyrrolizidine alkaloids (PAs) and their N-oxides (PANOs) are naturally occurring toxic plant metabolites that have attracted widespread attention due to their potential food safety hazards. In this study, an efficient analytical method for determining PAs/PANOs in bee pollen was established by combining accelerated solvent extraction (ASE) with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A magnetic covalent organic framework […]

ABSTRACT Caffeoylquinic acids are natural polyphenolic compounds with notable antioxidant and anti-inflammatory activities. This study developed and validated a sensitive high-performance liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of isochlorogenic acid A (ICQA) and 22 metabolites in serum. The method exhibited excellent sensitivity with limits of quantification of 0.625–1.25 µg/L (signal-to-noise ratio = 10), inter/intra-day precision below 15%, […]

ABSTRACT Quantifying hydrophilic B vitamins (B1 [(thiamine], B2 [riboflavin], and B3 [nicotinic acid]) in complex functional food matrices presents a significant challenge. Conventional sorbents, such as C18, often exhibit poor retention and low recoveries (50%–60%) due to weak hydrophilic interactions. To efficiently extract these target analytes from functional foods, a new sorbent with tailored hydrophilic affinity has been developed by […]

Are you interested in getting into spatial mass spectrometry but all the super expensive commercial technologies aren't doing it for you? Why don't you…..introducing Tappy-mode scanning ESI! What's that? Okay, they didn't invent it for this paper, but they explain it better than I can! Wait a minute. Is it just like this except really fast and with an electrospray plume in the […]

Background: Fragile X-associated Tremor/Ataxia Syndrome (FXTAS) is a late-onset neurodegenerative disorder caused by FMR1 premutation CGG repeat expansions (55-200 repeats). The epigenetic landscape of the FXTAS brain remains uncharacterized. We performed genome-wide DNA methylation profiling of postmortem prefrontal cortex tissue to identify differentially methylated positions (DMPs) and candidate genes, and sought protein-level support for a neuroinflammatory signal. Methods: DNA methylation […]

Cells release membrane-bound extracellular vesicles into the bloodstream laden with proteins that may reflect their physiological state. How this circulating EV proteome changes across life remains poorly understood. Identifying molecular signatures of aging in accessible biofluids could facilitate earlier intervention and monitoring of age-related disease. Many circulating aging proteome studies rely on affinity-based platforms which suffer from poor cross-species translation, […]

Endometriosis, a common inflammatory gynecological disorder affecting up to 10% of women worldwide, is characterized by the presence of endometrium-like tissue outside the uterus. Current diagnostic methods, such as ultrasound and MRI, effectively detect ovarian and deep endometriosis but fail to detect more common peritoneal type. Diagnosing peritoneal endometriosis currently necessitates invasive laparoscopy and histological confirmation. Despite numerous efforts, no […]