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ABSTRACT Cistanoside A (Cis A) has received more attention because of its potential in the treatment of osteoporosis. However, its in vivo process remains unknown. In this study, a simple liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed and validated for the determination of cistanoside A in rat plasma, urine, and feces. After prepared by protein precipitation, cistanoside A and IS […]

RNA modifications contribute to the regulatory and structural complexity of RNA molecules, influencing key biological processes. Nanopore direct RNA sequencing (DRS) enables the detection of these modifications at single-molecule resolution without chemical conversion. Oxford Nanopore Technologies recently introduced RNA004 sequencing chemistry and the Dorado basecaller, which improves accuracy and enables the identification of eight RNA modifications. However, the reliability of […]

Analyst, 2026, Accepted ManuscriptDOI: 10.1039/D6AN00299D, PaperYu-Nan Chen, Xiao-Long Hou, Ying Liu, Xuyang Shen, Yi-xuan Gu, Yue Yu, Xin Zheng, Ying-Lin Zhou, Xinxiang ZhangDue to the high molecular weight and polarity, glycans suffer low ionization efficiency in electrospray ionization mass spectrometry (ESI-MS). It is necessary to chemically derivatize them for MS response improvement….The content of this RSS Feed (c) The Royal […]

Analyst, 2026, Accepted ManuscriptDOI: 10.1039/D6AN00305B, PaperJiarui Zhang, Hui Miao, Jiale Liu, Xinyue Zhou, Yue Deng, Shuang Feng, Yalin Xi, Meiyun Shi, Lei YinAccurate quantification of hydrophilic monodisperse polyethylene glycol (PEG) derivatives in complex biological matrices remains challenging due to substantial matrix interference and poor retention using conventional chromatographic systems.This study presents an…The content of this RSS Feed (c) The Royal […]

Herein we uncover a relationship between histone succinylation and Jumonji (JmjC) domain-containing histone demethylases. We used quantitative proteomics and peptide pull-down assays to identify JmjC demethylases as candidate interactors with succinylated histone peptides. Succinyl-lysine peptides bind and inhibit the catalytic activity of JmjC demethylases in a dose-dependent manner. This includes KDM4D and KDM6B, which are responsible for the removal of […]

Analytical ChemistryDOI: 10.1021/acs.analchem.6c02808

Alcohol-associated liver disease (ALD) is a leading cause of liver-related morbidity and mortality. Although various omics approaches have revealed early metabolic alterations, individual datasets provide limited mechanistic insight. Here, we integrated RNA sequencing with mass spectrometry-based analyses to quantify gene expression, protein abundance, proteome and acetylome dynamics, and metabolic fluxes in livers of alcohol-fed mice. This multi-layered approach revealed extensive […]

Mass spectrometry-based proteomics enables in-depth investigation of protein phosphorylation, quantifying tens of thousands of phosphosites per sample following phosphopeptide enrichment. However, critical information on phosphosite occupancy (stoichiometry) is typically lost during the phospho-enrichment process. Here, we introduce SILAC-Site, a SILAC-based fractionation-free and chemical labelling-free workflow for direct phosphosite stoichiometry evaluation using stable isotope labeling and phosphatase treatment. By acquiring treated […]

Nano- and microplastics (NMPs), by-products of the fragmentation and degradation of plastic products, are ubiquitous environmental contaminants, yet their burden in pediatric immune tissues and functional consequences for developing immunity remain unknown. Here we report the first comprehensive characterization of NMPs in surgically excised pediatric tonsils (n = 30) using pyrolysis gas chromatography mass spectrometry (Py-GC/MS), Nile Red fluorescence microscopy, […]

Advances in Electrostatic Linear Ion Trap (ELIT) Charge Detection Mass Spectrometry (CDMS) over the past 10 years have revolutionized its use for analyzing very high-molecular-weight species such as protein complexes, viral vectors, vaccines, viruses, and amyloid fibrils. Nonetheless, ELIT-based CDMS has remained confined to a small number of specialized instrumentation groups, predominantly in academia, where large and complex home-built instruments […]

Interleukin-33 (IL-33) is a key cytokine in mast cell mediated immunity, promoting inflammatory cytokine production without inducing degranulation. Here, we compared IL-33 induced proteomic responses across three mast cell culture systems, Foetal Liver derived Mast Cells (FLMCs), Bone Marrow derived Mast Cells (BMMCs), and Peritoneal Mast Cells (PMCs), using quantitative data-independent acquisition mass spectrometry. Although baseline proteomes were largely conserved […]

We report the isolation and identification of a Paenibacillus polymyxa strain from the citizen science project; Swab and Send. Through whole genome sequencing we are able to describe the biosynthetic gene cluster of polymyxin A produced by P. polymyxa 1G (NCBI accession no. JBVPZV000000000), compare the pmxA, pmxB and pmxE genes to five other polymyxin genes encoding known polymyxin variants, […]

Most human genes produce multiple RNA isoforms, yet it remains unclear which isoforms are translated into stable, functional proteins. Long-read RNA-sequencing resolves full-length transcript structures and, when paired with mass spectrometry, can provide empirical evidence of isoform translation. Despite this opportunity, comprehensive workflows integrating isoform discovery, open reading frame prediction, peptide identification, and protein inference remain limited, leaving users to […]

Analytical ChemistryDOI: 10.1021/acs.analchem.6c00906

The leptomeninges (arachnoid and pia) are the inner layers of the meninges that envelope the brain. They define the borders of the subarachnoid space (SAS) where cerebrospinal fluid (CSF) circulates. Leptomeninges act as the barrier tissue and contribute to fluid exchange and immune function of the brain. These processes are identified as key factors in the development of neurodegenerative diseases. […]

Aims: Hypertrophic cardiomyopathy (HCM) is a genetic heart disease that leads to left ventricular hypertrophy, heart failure, and arrhythmias. Pathogenic missense variants in the gene myosin binding protein C (MYBPC3) cluster within its internal subdomains C3 and C6. The protein, myosin binding protein C (MyBP-C), expressing these variants, normally localizes to the myofilaments, leaving uncertainty regarding the mechanism(s) by which […]

A labile sulfur ligand in a three-cysteine-coordinated [2Fe−2S] cluster mediates sulfide sensing in NreB

ABSTRACT This study aimed to systematically characterize the chemical composition and develop a reliable strategy for identifying compounds in Allii macrostemonis Bulbus (AMB). This study employed an efficient, highly sensitive, and high-resolution analytical approach utilizing ultra-high-performance liquid chromatography-Quadrupole-Exactive Orbitrap mass spectrometry with parallel reaction monitoring scanning. A total of 130 compounds were preliminarily discovered in the AMB. The 130 compounds […]

 Just leaving this here so I can get back to it later in case we have some drug response data with a lot of variables! Super smart and some of the most honest writing. "Yes, in this dataset this other tool actually proves more sensitive"! I love it. The authors use both simulated and real datasets they generated using DIA and […]

 Official site link here! Highlights? 75 Hz in a Tribrid? That's rocket fast. Probably rocking the Excedion Pro's lower resolution Orbitrap scan rates? Unclear, but that would be the safe assumption. It looks like there will be 4 versions of ApeX aimed at different markets? There is a long held tradition for this vendor to take a big 'ol dump on the systems […]