- by MS ArticlesThe mitochondrial translocator protein (TSPO) was once proposed to mediate mitochondrial cholesterol import for steroid hormone biosynthesis, but genetic deletion studies in multiple models have refuted this role. Nevertheless, the idea that pharmacological ligands of TSPO can modulate steroid output continues to be invoked. One such compound, 19-Atriol (androst-5-ene-3{beta},17{beta},19-triol), was reported to inhibit progesterone synthesis via TSPO binding in MA-10 […]
- by MS ArticlesFor a special US HUPO sponsored episode highlighting the upcoming HUPO Meeting in Toronto, Ben and Ben sit down to talk with one of the organizers, Dr. Mathieu Lavallée-Adam, University of Ottawa.Keywords: bioinformatics; hockey; Toronto
- by MS ArticlesThe interaction between dissolved organic matter (DOM) and microbial communities is a critical yet understudied driver of biogeochemical cycling in aquatic ecosystems. Understanding these interactions is essential for elucidating the chemical and microbial dynamics that sustain ecosystem functionings. Here, we combined non-target ultra high-resolution mass spectrometry-based environmental metabolome analysis with microbiome analysis to conduct the first comprehensive investigation of DOM-microbe […]
- by MS ArticlesDNA methyltransferase 3A (DNMT3A) is a de novo DNA methyltransferase that is recurrently mutated in hematological malignancies and developmental disorders. The most prevalent mutation, R882H, compromises DNMT3A activity in a dominant-negative manner, but its precise biochemical mechanism has been debated. Here, we use paired deep mutational scanning of the wild-type and R882H-mutant proteins to systematically identify mutations on a massively […]
- by MS ArticlesAnalytical ChemistryDOI: 10.1021/acs.analchem.5c02995
- by MS ArticlesThe monosaccharide N-acetyl-glucosamine (GlcNAc), which dynamically modifies serine and threonine residues of nuclear and cytoplasmic proteins, is a key regulator of numerous biological processes. Investigating O-GlcNAc modification in vivo and in vitro remains challenging, making animal models essential for gaining powerful insights into this post-translational modification. In this study, we conducted a proteomic analysis for identifying O-GlcNAc-modified proteins in both […]
- by MS ArticlesABSTRACT Baihe Dihuang decoction (BDD), composed of Lilii Bulbus and Rehmanniae Radix, is a classical Chinese herbal formula. It demonstrates clinical applications in treating emotional disorders and anxiety. In this study, we characterized the chemical basis of BDD in vitro and elucidated its metabolic pathways, pharmacokinetic profiles, and tissue distribution in vivo. An ultra-high performance liquid chromatography coupled with quadrupole […]
- by MS ArticlesABSTRACT 1-Methyl-9-(3-pyridinylmethyl)-9H-pyrido[3,4-b] indole (HMYB) is a novel semi-synthetic alkaloid derived from β-carboline harmane, which has been proven to be effective in treatment of encopresis. But the pharmacokinetic characteristics of HMYB were not yet illustrated. In this study, three metabolites (harmane, harmol, and 7-OH-HMYB) of HMYB were isolated from rat urine after oral administration of HMYB and authenticated with nuclear magnetic […]
- by MS ArticlesChemistry – A European Journal, EarlyView.
- by MS ArticlesMicrobial breakdown of poly- and perfluoroalkyl substances (PFASs) remains poorly understood, often hindered by mass balance discrepancies and unexplained fluoride release. Here we identify a previously unrecognized PFAS biodefluorination mechanism mediated by mycothiol (MSH), a ubiquitous thiol antioxidant produced by Actinomycetota. Using high-resolution mass spectrometry and non-target analysis, MSH-mediated conjugation is unraveled as a dominant biotransformation pathway for two representative […]
- by MS ArticlesPublication date: Available online 24 September 2025Source: Journal of Chromatography AAuthor(s): Robert L. Ross, J. Michael Sutton, Keeley Murphy, Roberto Gamez, Ryan Cowley, Shanhua Lin, Min Du, Michael G. Bartlett
- by MS ArticlesPublication date: Available online 23 September 2025Source: International Journal of Mass SpectrometryAuthor(s): Jia-Xuan Yan, Wendy Zhong
- by MS ArticlesThis study introduces an analytical method for the determination of endocrine-disrupting chemicals (EDCs) in human urine samples, employing QuEChERS as a clean-up step for the first time in this biological matrix. Sample preparation was rigorously optimized through the evaluation of sample and final dilution volumes, balancing the need to reduce matrix interference without sacrificing sensitivity. High-performance liquid chromatography coupled with […]
- by MS ArticlesJournal of the American Chemical SocietyDOI: 10.1021/jacs.5c05797
- by MS ArticlesAnalyst, 2025, Accepted ManuscriptDOI: 10.1039/D5AN00705D, CommunicationLingna Zheng, Haiyu Lu, Qing Yang, Yixian Liu, Chunjing Chen, Qianzhen Li, Xiang GaoA high throughput method was developed for screening human MAT2A (hMAT2A) inhibitors by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS). The stable isotope deuterium-labeled S-adenosylmethionine (SAM-D3) was enzymatically synthesized by…The content of this RSS Feed (c) The Royal Society of Chemistry
- by MS ArticlesAnalyst, 2025, Accepted ManuscriptDOI: 10.1039/D5AN00565E, Paper Open Access   This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.Annabel Selina Jessica Eardley-Brunt, Thomas Mills, Rafail Kontronias, Giovanni Luigi de Maria, Keith Channon, The Oxford Acute Myocardial Infarction Study, Claire VallanceIntroduction: Analysis of small molecule metabolites found in blood plasma of patients undergoing treatment for STEMI has the potential […]
- by MS ArticlesAim: Enhance the understanding of intrinsic metabolic and oxidative stress vulnerability in normal-tension glaucoma (NTG) pathophysiology by targeted profiling of skin fibroblasts from NTG and control donors. Background: NTG is a primary open-angle glaucoma subtype characterised by glaucomatous neurodegeneration without elevated intraocular pressure. Increasing evidence links systemic metabolic and oxidative stress vulnerability to NTG pathophysiology, making non-ocular, somatic cells promising […]
- by MS ArticlesPROTEOMICS, Volume 25, Issue 17-18, Page 70-70, September 2025.
- by MS ArticlesABSTRACT This work focuses on the separation of oligonucleotides (ONs) in a free solution by capillary electrophoresis—mass spectrometry (CE-MS). Specifically, we evaluated a combination of separation in acidic background electrolytes (BGEs), nanospray ionization, and time-of-flight mass spectrometry in positive mode. A mixture of synthetic ONs, ranging in length from 15 to 78 nt, was employed as the test compounds. The key […]
- by MS ArticlesBackground Technological advancements in protein mass spectrometry have significantly enhanced analytical sensitivity and throughput, enabling single-cell proteomics by mass spectrometry (SCP-MS) to become reality. SCP-MS allows high-resolution analysis of cellular heterogeneity and function, bypassing bulk analysis limitations. Here, we used SCP-MS to document at the protein level the cellular diversity of mouse kidney cells. We further focused SCP-MS on low […]
Murray Mass Spectrometry Group
Research group of Kermit Murray at Louisiana State University where we use lasers for sampling and imaging and study the chemistry and physics of laser ablation.
