- by MS ArticlesYing Zhu is having a solid month for his CV! While the resolution might not wow people in the MALDI world, the depth sure should make up for it. 3,500 proteins quantified and more than 120 "pixels" analyzed per day (a pixel is a 50um x 50um square, though it looks like they can cut smaller than this. With this little material […]
- by MS ArticlesPublication date: Available online 21 December 2024Source: Journal of ProteomicsAuthor(s): Sandra Doua, Natacha Germain, Amale Geandrot, Cloé Defour, Aurélia Gay, Catherine Massoubre, Francois Lang, Bruno Estour, Bogdan Galusca
- by MS ArticlesWOW! Did I ever blow my initial take on this paper. Not a little, but by so much that I'm a little concerned about whether I have some level of impairment in my reading and/or comprehension right now. I'm tempted to write it off as flu like symptoms while moving my family to a new city in the winter, and […]
- by MS ArticlesPublication date: Available online 20 December 2024Source: Journal of Chromatography AAuthor(s): Xialin Luo, Jiaqi Tian, Qing Li, Zhonggan Jin, Xiaoyu Fan, Hong Zhang, Haitao Lv, Yi Ju
- by MS ArticlesThe advancement of crosslinking mass spectrometry (CLMS) has significantly enhanced the ability to study protein-protein interactions and complex biological systems. This study evaluates the performance of the Orbitrap Astral and Eclipse mass spectrometers in CLMS workflows, focusing on the identification of low-abundance crosslinked peptides. The comparison employed consistent liquid chromatography setups and experimental conditions, using Cas9 crosslinked with PhoX and […]
- by MS ArticlesRationale: Excess consumption of sugar- and fat-rich foods has heightened the prevalence of cardiometabolic disease, which remains a driver of cardiovascular disease- and type II diabetes-related mortality globally. Skeletal muscle insulin resistance is an early feature of cardiometabolic disease and is a precursor to diabetes. Insulin resistance risk varies with self-reported race, whereby, African-Americans have a greater risk of diabetes […]
- by MS ArticlesThe identification of MHC class I presented ligands by mass spectrometry (MS)-based immunopeptidomics is an essential tool to characterize antigen processing pathways and to define targets for tumor immunotherapies. However, existing sample preparation workflows typically require large sample inputs, limiting the applicability in high throughput drug screenings, kinetic immunopeptidome studies and for scare samples in clinical contexts. To address this […]
- by MS ArticlesThe combination of spatial metabolomics and spatial transcriptomics can reveal powerful connections between gene expression and metabolism in heterogeneous tissues, but section-to-section variability can convolute data integration. We present a novel method combining Desorption Electrospray Ionization Mass Spectrometry Imaging spatial metabolomics and spatial transcriptomics on the same tissue sections. We demonstrate this workflow on human breast and lung cancer tissues […]
- by MS ArticlesAnalytical ChemistryDOI: 10.1021/acs.analchem.4c04263
- by MS ArticlesToday you can buy a mass spec package and – as long as nothing breaks – and you take really good notes most people can be generating really good proteomics data.When it's not working – well….that's different. Troubleshooting still seems to require an expert or a lot of work and logical exclusion of components one at a time. If your fancy […]
- by MS ArticlesI might have honestly ranted about this preprint at some point last year, but it just came out in Nature Genetics. I think it counts as the all time high water mark for how much work scientists are willing to do to avoid mass spectrometrists. There is, by concentration more histone protein in each typical human cell than messenger RNA. Millions […]
- by MS ArticlesAnal. Methods, 2025, Accepted ManuscriptDOI: 10.1039/D4AY01670J, PaperSayan Gupta, Brandon Russell, Line G. Kristensen, Shawn M. Costello, James Tyler, Corie Y. Ralston, Behzad Rad, Susan MarquseeX-ray Footprinting Mass Spectrometry (XFMS) is a structural biology method that uses broadband X-rays for in situ hydroxyl radical labeling to map protein interactions and conformation in solution. However, while…The content of this RSS Feed (c) […]
- by MS ArticlesYersinia pestis is the etiologic agent of the plague. A hallmark of plague is subversion of the host immune response by disrupting host signaling pathways required for inflammation. This non-inflammatory environment permits bacterial colonization and has been shown to be essential for disease manifestation. Previous work has shown that Y. pestis inhibits phagocytosis and degranulation by neutrophils. Manipulation of these […]
- by MS ArticlesHuman neutrophils express numerous G protein-coupled receptors (GPCRs) of importance for immune regulation. Several of the functionally known neutrophil GPCRs, are not part of the human neutrophil proteome. To identify GPCRs not earlier shown to be expressed in human neutrophils, we utilized a microfluidic flow cell technique in combination with subcellular granule fractionation and liquid chromatography-tandem mass spectrometry (LC-MS/MS). This […]
- by MS ArticlesBackground and objectives: Brain capillary endothelial cells (BCECs) express transferrin receptor 1 (TfR1) to ensure sufficient iron transport into the brain across the blood-brain barrier (BBB). Our main objective was to examine adult mice subjected to dietary iron deficiency (ID) for possible changes in the content of TfR1 in BCECs and the influence thereof on the uptake and transport of […]
- by MS ArticlesSpatial proteomics studies the spatial distribution of proteins within cells or tissues, providing a new perspective for comprehending cellular processes and disease mechanisms. One important challenge of current spatial proteomics technologies is low resolution, resulting in multiple cells in each spatial proteomics spot. While methods have been proposed to infer the composition of potential cell types in such spots for […]
- by MS ArticlesAnal. Methods, 2025, 17,37-44DOI: 10.1039/D4AY01595A, PaperHan Zhang, Yinran Xiong, Xiaonan Shi, Lijia Zhu, Qiong Wu, Ting Wu, Yiping DuA newly developed algorithm based on isotope pattern changes and non-negative least squares calculation was developed for quantitative analysis of deamidation in peptides with no reference standards.The content of this RSS Feed (c) The Royal Society of Chemistry
- by MS ArticlesResistance to endocrine therapy (ET) is common in estrogen receptor (ER) positive breast cancer. Multiple studies have demonstrated that upregulation of MAPK signaling pathways contributes to ET resistance. Herein we show that vandetanib treatment enhances sensitivity to ET in ET-sensitive and -resistant ER+ breast cancer models. Using a CRISPR knockout model, we demonstrate that vandetanib effects are partially mediated by […]
- by MS ArticlesDespite the availability of new therapies for cervical cancer, innovative strategies are essential to address challenges related to drug resistance and high toxicity. The present study focuses on the metabolic profiling of cervical carcinoma using non-targeted metabolomics approach using liquid chromatography-mass spectrometry. Our study identified over 100 metabolites in cervical tissue samples (both cancerous and adjacent normal) using HILIC and […]
- by MS ArticlesThe progression of the cell cycle is regulated by the expression of specific genes and fluctuations in cellular metabolic states. Previous research has employed cell cycle-based transcriptomics, proteomics, and metabolomics analyses to identify cell cycle-dependent changes at the gene expression, protein, and metabolic levels. However, the role of protein compartmentalization in regulating protein function, coupled with evidence that metabolic enzymes […]
Murray Mass Spectrometry Group
Research group of Kermit Murray at Louisiana State University where we use lasers for sampling and imaging and study the chemistry and physics of laser ablation.
