- by MS ArticlesSingle-cell mass spectrometry (MS) recently emerged with unprecedented sensitivity to characterize cellular proteomes. Still, limited access to sensitive and fast mass spectrometers impedes its broad adoption, due chiefly to budget constraints. Here, we democratize single-cell MS to a budget-conscious alternative with contemporary performance. A custom-built inexpensive capillary electrophoresis (CE) platform electrophoresis-correlatively (Eco) sorted peptide m/z signals. Detection re-employed a decade-old, […]
- by MS ArticlesJournal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00047
- by MS ArticlesABSTRACT Polygonum orientale L. (PO) represents significant bioactivities in treating myocardial ischemia (MI); however, its underlying mechanisms remain unclear. This study aims to elucidate PO's potential mechanisms in MI using an integrated approach that combines UHPLC–Q-Exactive Orbitrap HRMS, network pharmacology, and RNA-sequencing (RNA-seq). Initially, the chemical constituents of PO were identified using UHPLC–Q-Exactive Orbitrap HRMS. Subsequently, network pharmacology, molecular docking, […]
- by MS ArticlesABSTRACT Methylene blue (MB), a phenothiazine derivative, is currently under clinical trials for Alzheimer's disease (AD) due to its potential to inhibit tau aggregation, a key pathological process in AD. In this study, we developed and qualified a rapid and reliable liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-qTOF-MS) method for the quantification of MB in mouse plasma and brain samples. Chromatographic […]
- by MS ArticlesEnhancer RNAs (eRNAs) are transcribed by RNA polymerase II during enhancer activation but are typically rapidly degraded in the nucleus. During states of reduced RNA surveillance, however, eRNAs and other similar "noncoding" RNAs, including for example upstream antisense RNAs, are stabilized, and some are exported to the cytoplasm and can even be found on polysomes. Here, we report unexpectedly that […]
- by MS ArticlesI even signed it and 99% of my interaction with Skyline is complaining about how I'm not smart enough to ever make it do what I want.Please drop in here and sign the stuff!
- by MS ArticlesABSTRACT Rationale To perform native mass spectrometry (MS) studies, there are a limited number of volatile and electrospray ionization (ESI)-MS compatible solutions, such as ammonium bicarbonate and ammonium acetate (AA). These solutions could induce the unfolding of proteins due to the formation of CO2 bubbles or induced acidification during ESI. Hence, it was important to introduce a buffer suitable to […]
- by MS ArticlesABSTRACT Rationale Edaravone (EDA) is a radical scavenger and an antioxidant drug approved to treat amyotrophic lateral sclerosis and used as a research tool to explore treatment of neurodegenerative diseases and cancers. It is also a reactive agent, known as PMP (1-phenyl-3-methyl-5-pyrazolone), used for the analysis of polysaccharides composition. EDA can react with sugars and aromatic aldehydes. In this context, […]
- by MS ArticlesSynaptic neurotransmission is a critical hallmark of brain activity and one of the first processes to be affected in neural diseases. Monitoring this process, and in particular synaptic vesicle recycling, in living cells has been instrumental in unraveling mechanisms responsible for neurotransmitter release. However, currently available reporters suffer from major limitations such large probe size or lack of suitability for […]
- by MS ArticlesD-aspartate is an endogenous agonist of NMDA and mGlu5 receptors, with a distinctive spatiotemporal expression profile that peaks in the prenatal and early postnatal brain. This suggests a critical role for D-aspartate metabolism in modulating neurodevelopmental processes linked to glutamatergic neurotransmission. However, the precise mechanisms through which D-aspartate exerts its effects remain unclear. To elucidate the molecular pathways orchestrated by […]
- by MS ArticlesPublication date: Available online 19 April 2025Source: Analytica Chimica ActaAuthor(s): Mudassir Shah, Linlin Wang, Lei Guo, Chengyi Xie, Thomas Ka-Yam Lam, Lingli Deng, Xiangnan Xu, Jingjing Xu, Jiyang Dong, Zongwei Cai
- by MS Articles….yeah….I'll just leave this here.
- by MS ArticlesPublication date: Available online 19 April 2025Source: Analytica Chimica ActaAuthor(s): Na Li, Xialian Cui, Yingjie Lu, Fang Zhang, Su Guo, Yue Su, Yinlong Guo
- by MS ArticlesJournal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00038
- by MS ArticlesJournal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00030
- by MS ArticlesJournal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00015
- by MS ArticlesABSTRACT Mass spectrometry-based proteomics is essential for advancing preventive and personalised medicine. Technological advancements have greatly increased both the number and sensitivity of spectra generated in a single experiment. Traditionally, spectra are identified using database search engines that depend on large and continuously expanding databases. This expansion enlarges the search space, posing challenges for controlling the false discovery rate in […]
- by MS ArticlesEnvironmental Science & Technology LettersDOI: 10.1021/acs.estlett.5c00258
- by MS ArticlesMetabolite annotation is a critical step in discovery metabolomics, but remains a significant challenge. In this study, the accuracy of metabolite annotation was systematically evaluated by leveraging the proposed strategies for simulation of tandem mass spectrometry (MS/MS) data from high-resolution mass spectrometry (HRMS) and then construction of a large-scale virtual database. Furthermore, various similarity scoring methods were comprehensively compared to […]
- by MS ArticlesEpithelial cancers such as stomach and ovary cancer tend to metastasize to the peritoneum, often leading to intractable disease and poor survival. Currently, the mechanisms that enable gastric cancer cells to penetrate the mesothelium, and to implant, invade, and survive in the peritoneal niche are poorly understood. To investigate these mechanisms, we developed a novel human peritoneal explant model. Briefly, […]
Murray Mass Spectrometry Group
Research group of Kermit Murray at Louisiana State University where we use lasers for sampling and imaging and study the chemistry and physics of laser ablation.
