Mass spectrometry RSS feeds from journals and social media.
- by MS ArticlesYing Zhu is having a solid month for his CV! While the resolution might not wow people in the MALDI world, the depth sure should make up for it. 3,500 proteins quantified and more than 120 "pixels" analyzed per day (a pixel is a 50um x 50um square, though it looks like they can cut smaller than this. With this little material and an eye on throughput – the fancy tools on the Eclipse for MS3 and Real Time Search didn't improve […]
- by MS ArticlesPublication date: Available online 21 December 2024Source: Journal of ProteomicsAuthor(s): Sandra Doua, Natacha Germain, Amale Geandrot, Cloé Defour, Aurélia Gay, Catherine Massoubre, Francois Lang, Bruno Estour, Bogdan Galusca
- by MS ArticlesWOW! Did I ever blow my initial take on this paper. Not a little, but by so much that I'm a little concerned about whether I have some level of impairment in my reading and/or comprehension right now. I'm tempted to write it off as flu like symptoms while moving my family to a new city in the winter, and hoping it isn't a brain tumor, but – if you saw that post it was way off. I'm tempted to leave […]
- by MS ArticlesPublication date: Available online 20 December 2024Source: Journal of Chromatography AAuthor(s): Xialin Luo, Jiaqi Tian, Qing Li, Zhonggan Jin, Xiaoyu Fan, Hong Zhang, Haitao Lv, Yi Ju
- by MS ArticlesThe advancement of crosslinking mass spectrometry (CLMS) has significantly enhanced the ability to study protein-protein interactions and complex biological systems. This study evaluates the performance of the Orbitrap Astral and Eclipse mass spectrometers in CLMS workflows, focusing on the identification of low-abundance crosslinked peptides. The comparison employed consistent liquid chromatography setups and experimental conditions, using Cas9 crosslinked with PhoX and DSSO as quality control samples. Results demonstrated that the Astral analyzer outperformed the Eclipse, achieving over 40% more unique residue […]
- by MS ArticlesRationale: Excess consumption of sugar- and fat-rich foods has heightened the prevalence of cardiometabolic disease, which remains a driver of cardiovascular disease- and type II diabetes-related mortality globally. Skeletal muscle insulin resistance is an early feature of cardiometabolic disease and is a precursor to diabetes. Insulin resistance risk varies with self-reported race, whereby, African-Americans have a greater risk of diabetes development relative to their White counterparts. Self-reported race is strongly associated with mitochondrial DNA (mtDNA) haplogroups, and previous reports have […]
- by MS ArticlesThe identification of MHC class I presented ligands by mass spectrometry (MS)-based immunopeptidomics is an essential tool to characterize antigen processing pathways and to define targets for tumor immunotherapies. However, existing sample preparation workflows typically require large sample inputs, limiting the applicability in high throughput drug screenings, kinetic immunopeptidome studies and for scare samples in clinical contexts. To address this challenge, we developed Mild Acid Elution in a Tip (MAETi), a streamlined and antibody-free approach for high-sensitivity MHC-I immunopeptidome profiling […]
- by MS ArticlesThe combination of spatial metabolomics and spatial transcriptomics can reveal powerful connections between gene expression and metabolism in heterogeneous tissues, but section-to-section variability can convolute data integration. We present a novel method combining Desorption Electrospray Ionization Mass Spectrometry Imaging spatial metabolomics and spatial transcriptomics on the same tissue sections. We demonstrate this workflow on human breast and lung cancer tissues identifying correlations between metabolites and cancer-related RNA transcripts.
- by MS ArticlesAnalytical ChemistryDOI: 10.1021/acs.analchem.4c04263
- by MS ArticlesToday you can buy a mass spec package and – as long as nothing breaks – and you take really good notes most people can be generating really good proteomics data.When it's not working – well….that's different. Troubleshooting still seems to require an expert or a lot of work and logical exclusion of components one at a time. If your fancy neural network machine intelligence thing that turns those spectra into biology decides to have a headache and not work? Well, […]
- by MS ArticlesI might have honestly ranted about this preprint at some point last year, but it just came out in Nature Genetics. I think it counts as the all time high water mark for how much work scientists are willing to do to avoid mass spectrometrists. There is, by concentration more histone protein in each typical human cell than messenger RNA. Millions of copies of these little proteins. They are super evolutionarily conserved, though, so the differences between important histone A and […]
- by MS ArticlesAnal. Methods, 2025, Accepted ManuscriptDOI: 10.1039/D4AY01670J, PaperSayan Gupta, Brandon Russell, Line G. Kristensen, Shawn M. Costello, James Tyler, Corie Y. Ralston, Behzad Rad, Susan MarquseeX-ray Footprinting Mass Spectrometry (XFMS) is a structural biology method that uses broadband X-rays for in situ hydroxyl radical labeling to map protein interactions and conformation in solution. However, while…The content of this RSS Feed (c) The Royal Society of Chemistry
- by MS ArticlesYersinia pestis is the etiologic agent of the plague. A hallmark of plague is subversion of the host immune response by disrupting host signaling pathways required for inflammation. This non-inflammatory environment permits bacterial colonization and has been shown to be essential for disease manifestation. Previous work has shown that Y. pestis inhibits phagocytosis and degranulation by neutrophils. Manipulation of these key vesicular trafficking pathways suggests that Y. pestis influences EV secretion, cargo selection, trafficking, and/or maturation. Our goal was to […]
- by MS ArticlesHuman neutrophils express numerous G protein-coupled receptors (GPCRs) of importance for immune regulation. Several of the functionally known neutrophil GPCRs, are not part of the human neutrophil proteome. To identify GPCRs not earlier shown to be expressed in human neutrophils, we utilized a microfluidic flow cell technique in combination with subcellular granule fractionation and liquid chromatography-tandem mass spectrometry (LC-MS/MS). This approach added the hydroxy-carboxylic acid 3 receptor (HCA3R) to the human neutrophil proteome. The beta-oxidation intermediate 3-hydroxy-octanoic acid (3-OH-C8) is […]
- by MS ArticlesBackground and objectives: Brain capillary endothelial cells (BCECs) express transferrin receptor 1 (TfR1) to ensure sufficient iron transport into the brain across the blood-brain barrier (BBB). Our main objective was to examine adult mice subjected to dietary iron deficiency (ID) for possible changes in the content of TfR1 in BCECs and the influence thereof on the uptake and transport of high-affinity anti-transferrin receptor IgG1 antibodies (clone Ri7217). Material and methods: We subjected adult, female mice to dietary ID for 8 […]
- by MS ArticlesSpatial proteomics studies the spatial distribution of proteins within cells or tissues, providing a new perspective for comprehending cellular processes and disease mechanisms. One important challenge of current spatial proteomics technologies is low resolution, resulting in multiple cells in each spatial proteomics spot. While methods have been proposed to infer the composition of potential cell types in such spots for spatial transcriptomics, the depressed correlation and divergent quantification between transcriptome and proteome limits their capability in spatial proteomics. To enhance […]
- by MS ArticlesAnal. Methods, 2025, 17,37-44DOI: 10.1039/D4AY01595A, PaperHan Zhang, Yinran Xiong, Xiaonan Shi, Lijia Zhu, Qiong Wu, Ting Wu, Yiping DuA newly developed algorithm based on isotope pattern changes and non-negative least squares calculation was developed for quantitative analysis of deamidation in peptides with no reference standards.The content of this RSS Feed (c) The Royal Society of Chemistry
- by MS ArticlesResistance to endocrine therapy (ET) is common in estrogen receptor (ER) positive breast cancer. Multiple studies have demonstrated that upregulation of MAPK signaling pathways contributes to ET resistance. Herein we show that vandetanib treatment enhances sensitivity to ET in ET-sensitive and -resistant ER+ breast cancer models. Using a CRISPR knockout model, we demonstrate that vandetanib effects are partially mediated by RET receptor tyrosine kinase. Vandetanib treatment alters the gene expression program of ER+ breast cancer cells resulting in a less […]
- by MS ArticlesDespite the availability of new therapies for cervical cancer, innovative strategies are essential to address challenges related to drug resistance and high toxicity. The present study focuses on the metabolic profiling of cervical carcinoma using non-targeted metabolomics approach using liquid chromatography-mass spectrometry. Our study identified over 100 metabolites in cervical tissue samples (both cancerous and adjacent normal) using HILIC and reversed-phase chromatography in the positive and negative ionization modes. The major metabolic alterations included changes in glycolysis, the citric acid […]
- by MS ArticlesThe progression of the cell cycle is regulated by the expression of specific genes and fluctuations in cellular metabolic states. Previous research has employed cell cycle-based transcriptomics, proteomics, and metabolomics analyses to identify cell cycle-dependent changes at the gene expression, protein, and metabolic levels. However, the role of protein compartmentalization in regulating protein function, coupled with evidence that metabolic enzymes can localize to the nucleus and influence chromatin states, suggests that fluctuations in nuclear metabolism may play a role in […]
- by MS ArticlesAs part of the US HUPO sponsored "Philly Express" series highlighting speakers at the upcoming 2025 US HUPO meeting in Philadelphia, Ben and Ben sit down to talk with Dr. Tara Tracy, Buck Institute for Research on Aging.
- by MS ArticlesAging results in a progressive decline in physiological function due to the deterioration of essential biological processes, such as transcription and RNA splicing, ultimately increasing mortality risk. Although proteomics is emerging as a powerful tool for elucidating the molecular mechanisms of aging, existing studies are constrained by limited proteome coverage and only observe a narrow range of lifespan. To overcome these limitations, we integrated the Orbitrap Astral Mass Spectrometer with the multiplex tandem mass tag (TMT) technology to profile the […]
- by MS ArticlesProteome-wide non-cleavable crosslink identification with MS Annika 3.0 reveals the structure of the C. elegans Box C/D complex
- by MS ArticlesOdor prediction of whiskies based on their molecular composition
- by MS ArticlesAnalytical ChemistryDOI: 10.1021/acs.analchem.4c05341