Mass spectrometry RSS feeds from journals and social media.
- by MS ArticlesAlkB homolog 1 (ALKBH1) is a member of the AlkB family of Fe(II) and -ketoglutarate (-KG)-dependent dioxygenases, known for its enzymatic activity on various nucleic acid substrates. Its known targets include N1-methyladenosine (m1A), N6-methyladenosine (m6A), N3-methylcytidine (m3C), and 5-methylcytosine (m5C) in RNA, as well as N6-methyladenine (N6-mA, 6mA) in DNA and the histone protein H2A. Moreover, dysregulation or dysfunction of ALKBH1 has been implicated in a broad spectrum of human diseases. In order to shed further light on the substrate […]
- by MS ArticlesABSTRACT Rationale Flavonoids are phenolic compounds with many health-benefiting properties. However, differentiating different types of flavonoids and their isomers is challenging due to their highly similar structures of various subtypes and different numbers and sites of substituents. Timely quality evaluation of flavonoid-based products is currently almost impossible. Methods An ambient ionization method of direct analysis in real time (DART) ion source and tandem mass spectrometry (MS) was used to characterize the fine structures of flavonoids. Different flavonoid subtypes and their […]
- by MS ArticlesPublication date: Available online 7 November 2025Source: Journal of ProteomicsAuthor(s): Orlando Morales-Tarré, Xitlally Popa Navarro, Sergio Encarnación-Guevara
- by MS ArticlesPublication date: Available online 7 November 2025Source: Journal of Mass Spectrometry and Advances in the Clinical LabAuthor(s): J. Brandsma, J.W. Thompson, K.L. Schully, J.G. Chenoweth, P. Genzor, S. Krishnan, D.A. Striegel, L.St. John-Williams, A. Moseley, G. Oduro, N. Adams, T. Vantha, E.L. Tsalik, C.W. Woods, D.V. Clark
- by MS ArticlesFormalin-fixed paraffin-embedded (FFPE) samples are essential for clinical research and spatial proteomics (SP) but are technically challenging to analyze by liquid-chromatography coupled mass-spectrometry (LC-MS) due to losses during protein extraction, evaporation during decrosslinking and low-throughput sample preparation methods. Here, we present a streamlined micro-FFPE proteomics sample preparation protocol for laser-capture micro-dissection (LMD) that enables scalable and high-throughput processing in 96-well format, completing the entire process from tissue lysis to tryptic peptide mixtures on LC-MS-ready Evotips in just 2-hours. Our method […]
- by MS ArticlesObjective: Primary liver cancer is a leading cause of cancer-related mortality and harbors recurrent mutations in chromatin regulators such as BRCA1-associated protein 1 (BAP1), yet their functional impact remains unclear. We investigated how BAP1 deficiency affects liver homeostasis and tumorigenesis to clarify its functional role. Design: We employed inducible, liver-specific BAP1 knockdown in mice subjected to diet-induced metabolic stress (including rescue experiments), alongside autochthonous hydrodynamic CRISPR models, and profiled livers by RNA-seq, immunohistochemistry, and mass spectrometry-based lipidomics. Complementary mechanistic assays […]
- by MS ArticlesPublication date: Available online 7 November 2025Source: TrAC Trends in Analytical ChemistryAuthor(s): David Fabregat-Safont, Francisco Madrid-Gambin, Alex Gomez-Gomez, Élida Alechaga, Gabriel Gil-Gómez, Óscar J. Pozo
- by MS ArticlesAnalytical ChemistryDOI: 10.1021/acs.analchem.5c04075
- by MS ArticlesAnalytical ChemistryDOI: 10.1021/acs.analchem.5c05632
- by MS ArticlesAnalytical ChemistryDOI: 10.1021/acs.analchem.5c05341
- by MS ArticlesPublication date: Available online 6 November 2025Source: Journal of Chromatography AAuthor(s): Hannah Schanzmann, Veronika Ruzsanyi, Parviz Ahmad-Nejad, Ursula Telgheder, Stefanie Sielemann
- by MS ArticlesSuitability of prolonged meloxicam treatment in mice seems limited due to unfavorable pharmacokinetics, side effects, and impact on home-cage behaviors
- by MS ArticlesPublication date: Available online 6 November 2025Source: International Journal of Mass SpectrometryAuthor(s): Kenzo Hiraoka, Stephanie Rankin-Turner, Dilshadbek T. Usmanov, Satoshi Ninomiya
- by MS ArticlesUntargeted tandem mass spectrometry (MS/MS)-based metabolomics enables broad characterization of small molecules in complex samples, yet the majority of spectra in a typical experiment remain unannotated, limiting biological interpretation. Reference data-driven (RDD) metabolomics addresses this gap by contextualizing spectra through comparison to curated, metadata-annotated reference datasets, allowing inference of spectrum origins without requiring exact structural identification. Here, we present an open-source RDD metabolomics platform comprising a user-friendly web application and a Python software package that perform RDD analyses directly from […]
- by MS ArticlesMass spectrometry imaging (MSI) is emerging as a powerful tool for uncovering the distribution of metabolites in the tumor microenvironment and studying tumor metabolism in vivo. However, to date, MSI of primary patient biobanked tissues contextualized by patient data has been limited to peptides, proteins, and glycans – with few examples for metabolites. This is because most biobanked fresh-frozen tissue required for spatial metabolomics is embedded in optimal cutting temperature compound (OCT), which introduces high-abundance polymeric interferents. Herein, we use […]
- by MS ArticlesRefining EI-MS library search results through atomic-level insights
- by MS ArticlesClick-linking: a cell-compatible protein crosslinking method based on click chemistry
- by MS ArticlesExosomes are extracellular vesicles (EVs) that carry bioactive molecules from a cell of origin and may alter gene expression of the acceptor cell via binding to cell surface receptors and/or delivering their cargo into the cell. Tumor-associated macrophages (TAMs) can increase or decrease tumor growth through various mechanisms, but the impact of TAM-EVs on tumors has been difficult to elucidate due to challenges in isolating and culturing TAMs for EV purification. In this study, we set out to uncover the […]
- by MS ArticlesJournal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00256
- by MS ArticlesAnalytical ChemistryDOI: 10.1021/acs.analchem.5c05564
- by MS ArticlesAnalytical ChemistryDOI: 10.1021/acs.analchem.5c05114
- by MS ArticlesAnalytical ChemistryDOI: 10.1021/acs.analchem.5c03549
- by MS ArticlesNOS1AP (CAPON) is an adaptor protein of neuronal nitric oxide synthase (nNOS) correlated with Alzheimers disease progression, making it an attractive yet unexplored therapeutic target. To assess its chemical tractability, we employed affinity selection-mass spectrometry (AS-MS) to screen approximately 10,000 small molecules for CAPON binding, identifying 52 initial hits. These compounds were further evaluated for true binding interactions and potential autofluorescence or quenching effects using the Dianthus platform. Five compounds were selected for quantitative affinity determination by microscale thermophoresis (MST). […]
- by MS ArticlesPublication date: Available online 6 November 2025Source: Journal of Chromatography AAuthor(s): Guangyu Dong, Linchun Nie, Jiawei Liao, Dejing Meng, Wenjing Liu, Rui Yang, Haobo Yang, Qingxiang Zhou
- by MS ArticlesPublication date: January 2026Source: International Journal of Mass Spectrometry, Volume 519Author(s): Matjaž Finšgar, Katja Andrina Varda