Mass Spectrometry Applications

One of the most enjoyable aspects of instrument development is using these instruments in new ways and on new systems. This usually involves collaboration with experts in the application area and always requires learning new things.

In this work, we examined proteomic responses to natural and artificial diets in the honey bee fat body, a tissue with central nutrient storage and metabolic functions. Bees were fed protein diets of natural pollen, a commercial plant-based diet used by beekeepers that does not contain pollen, and two novel cyanobacteria diets comprising dried or fresh laboratory-grown spirulina.

V.A. Ricigliano, C. Dong, L.T. Richardson, F. Donnarumma, S.T. Williams, T. Solouki, K.K. Murray, Honey Bee Proteome Responses to Plant and Cyanobacteria (blue-green algae) Diets, ACS Food Science & Technology, 1 (2021) 17-26.

Fungicides can penetrate the outer skin of fruit and diffuse into the interior region. Mass spectrometry analysis of fungicide treated apples is commonly conducted using the entirety of the fruit, which results in loss of localization information and does not provide any information about the rate of penetration. We developed a MALDI imaging method that can provide localization of pesticide and laser ablation sampling for electrospray ionization mass spectrometry.

I. Pereira, B. Banstola, K. Wang, F. Donnarumma, B.G. Vaz, K.K. Murray, Matrix-Assisted Laser Desorption Ionization Imaging and Laser Ablation Sampling for Analysis of Fungicide Distribution in Apples, Anal. Chem., 91 (2019) 6051-6056.

The goal of this project was to develop mass spectrometry fingerprinting methods for the identification of shrimp species. We used a mass spectrometry system originally developed for bacteria identification and adapted it to shrimp.

Salla, Murray, “Matrix-Assisted Laser Desorption Ionization Mass Spectrometry for Identification of Shrimp.” Anal. Chim. Acta 2013, 794, 55–59.

Alligators and crocodiles live in environments with large concentrations of pathogenic microbes, yet their wounds typically heal without infection. This is thought to be due to cationic peptides in their white blood cells. In a collaboration with Prof. Mark Merchant at McNeese State University, we developed methods to isolate peptides in alligator blood with analysis using a mass spectrometry and proteomics based approach. One and two-dimensional gel electrophoresis, reversed phase high-performance liquid chromatography, and nano-electrospray tandem mass spectrometry were used for biomolecule identification.

L.N.F. Darville, M.E. Merchant, V. Maccha, V.R. Siddavarapu, A. Hasan, K.K. Murray, Isolation and determination of the primary structure of a lectin protein from the serum of the American alligator (Alligator mississippiensis). Comp. Biochem. Physiol. B, Biochem. Mol. Biol., 161 (2012) 161-169.

L.N.F. Darville, M.E. Merchant, A. Hasan, K.K. Murray, Proteome analysis of the leukocytes from the American alligator (Alligator mississippiensis) using mass spectrometry., Comp. Biochem. Physiol. Part D Genomics Proteomics, 5 (2010) 308-316.

• ASMS 2022: Metaproteomics of the Honeybee Gut
• Multimodal Label‐Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers
• Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer
• Honey Bee Proteome Responses to Plant and Cyanobacteria (blue-green algae) Diets
• Label-free lipidome study of PVT of rat brain with post-traumatic stress injury by Raman imaging
• Matrix-Assisted Laser Desorption Ionization Imaging and Laser Ablation Sampling for Analysis of Fungicide Distribution in Apples
• Matrix-assisted laser desorption ionization mass spectrometry for identification of shrimp
• A mass spectrometry approach for the study of deglycosylated proteins

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