- Database searches with semi-specific enzyme traditionally have low sensitivity due to the large search space. Except, Mascot Server 3.0 includes MS²PIP, which boosts sensitivity. We've compared the results to a recent FragPipe publication.https://www.matrixscience.com/blog/mascot-vs-fragpipe-uncovering-endogenous-proteolytic-processing.html#proteomics #massspec
- Mascot Newsletter, December 2024:Predicted fragment intensities with Mascot Server 3.0.Self-ligating surface receptor and CD8+ T-cells.Saving search results as HTML.https://www.matrixscience.com/nl/202412/newsletter.html#proteomics #massspec
- Mascot Daemon is the automation client bundled with Mascot Server. The Quantitation Summary report is an easy way to export protein expression data for complex experiments.https://youtu.be/4Ds59gYhL4Q#proteomics #massspec
- Check out how Mascot Server 3.0 and MS²PIP work together to boost protein and peptide identifications.https://youtu.be/O7LSREqiW8o #proteomics #MassSpec
- Mascot Server 3.0 produces excellent results with Thermo Orbitrap instruments. We processed a DDA LFQ benchmark by Bart van Puyvelde et al. using MS2PIP model HCD2021. Old Mascot: 4667 protein hits; Mascot 3.0: 5007 protein hits.https://www.matrixscience.com/blog/best-ms2pip-model-for-thermo-orbitrap.html#proteomics #MassSpec
- Creating a list of confidently identified proteins is easy.1) Run the database search in Mascot.2) Version 3.0 defaults to 1% sequence FDR. Filter out one-hit wonders in Report Builder, if desired.4) Export as CSV — done.https://www.matrixscience.com/help/creating_a_list_of_confidently_identified_proteins.html#proteomics #MassSpec
- #TODO Write better code so you don't have to come back to so many TODO's#MassSpectrometry #python #MS #massspec #dataanalytics #analytics #science #chemistry #analyticalchemistry #matchms #programming #programminghumor
- Mascot Newsletter, November 2024:Predicted retention times in Mascot Server 3.0.N-terminal methylation of Physcomitrella organellar proteins.Mascot Server 3.0 compatibility with third-party applications.https://www.matrixscience.com/nl/202411/newsletter.html #proteomics #MassSpec #MassSpectrometry
- Reliable scoring of peptide matches is vitally important when they are used as input to machine learning. Mascot probabilistic scoring is the ideal foundation, now documented in greater detail than ever before. #proteomics #MassSpec #MassSpectrometry https://www.matrixscience.com/help/scoring_help.html
- Looking for opinions on which 193 nm excimer laser ablation unit to get to couple with our Element 2 for high-res analysis trace and minor elements in carbonates (esp. speleothems). Trying to choose between RESOlution or ESL. If any geochemists/LAICPMS afficonados on here have OPINIONS on either of these (good or bad), please drop me a line! #Geochemistry #LAICPMS #Laser #MassSpec #MassSpectrometry
- Protein inference is a critical and often misunderstood step. Mascot implements a novel algorithm that uses information from both unique and shared peptides to build a complete picture of the sample content. #proteomics #MassSpec #MassSpectrometryhttps://www.matrixscience.com/help/protein_inference.html
- Mascot Newsletter, October 2024: Mascot Server 3.0 is released. Thermal proteome profiling and affinity ultrafiltration for analysing natural products. Mascot at HUPO 2024. #proteomics #MassSpec #MassSpectrometryhttps://www.matrixscience.com/nl/202410/newsletter.html
- Database searches with semi-specific enzyme traditionally have low sensitivity due to the large search space. Except, Mascot Server 3.0 includes MS²PIP, which boosts sensitivity. We've compared the results to a recent FragPipe publication.https://www.matrixscience.com/blog/mascot-vs-fragpipe-uncovering-endogenous-proteolytic-processing.html#proteomics #massspec
- Mascot Newsletter, December 2024:Predicted fragment intensities with Mascot Server 3.0.Self-ligating surface receptor and CD8+ T-cells.Saving search results as HTML.https://www.matrixscience.com/nl/202412/newsletter.html#proteomics #massspec
- Mascot Daemon is the automation client bundled with Mascot Server. The Quantitation Summary report is an easy way to export protein expression data for complex experiments.https://youtu.be/4Ds59gYhL4Q#proteomics #massspec
- Check out how Mascot Server 3.0 and MS²PIP work together to boost protein and peptide identifications.https://youtu.be/O7LSREqiW8o #proteomics #MassSpec
- Mascot Server 3.0 produces excellent results with Thermo Orbitrap instruments. We processed a DDA LFQ benchmark by Bart van Puyvelde et al. using MS2PIP model HCD2021. Old Mascot: 4667 protein hits; Mascot 3.0: 5007 protein hits.https://www.matrixscience.com/blog/best-ms2pip-model-for-thermo-orbitrap.html#proteomics #MassSpec
- Creating a list of confidently identified proteins is easy.1) Run the database search in Mascot.2) Version 3.0 defaults to 1% sequence FDR. Filter out one-hit wonders in Report Builder, if desired.4) Export as CSV — done.https://www.matrixscience.com/help/creating_a_list_of_confidently_identified_proteins.html#proteomics #MassSpec
- #TODO Write better code so you don't have to come back to so many TODO's#MassSpectrometry #python #MS #massspec #dataanalytics #analytics #science #chemistry #analyticalchemistry #matchms #programming #programminghumor
- Mascot Newsletter, November 2024:Predicted retention times in Mascot Server 3.0.N-terminal methylation of Physcomitrella organellar proteins.Mascot Server 3.0 compatibility with third-party applications.https://www.matrixscience.com/nl/202411/newsletter.html #proteomics #MassSpec #MassSpectrometry
- Reliable scoring of peptide matches is vitally important when they are used as input to machine learning. Mascot probabilistic scoring is the ideal foundation, now documented in greater detail than ever before. #proteomics #MassSpec #MassSpectrometry https://www.matrixscience.com/help/scoring_help.html
- Looking for opinions on which 193 nm excimer laser ablation unit to get to couple with our Element 2 for high-res analysis trace and minor elements in carbonates (esp. speleothems). Trying to choose between RESOlution or ESL. If any geochemists/LAICPMS afficonados on here have OPINIONS on either of these (good or bad), please drop me a line! #Geochemistry #LAICPMS #Laser #MassSpec #MassSpectrometry
- Protein inference is a critical and often misunderstood step. Mascot implements a novel algorithm that uses information from both unique and shared peptides to build a complete picture of the sample content. #proteomics #MassSpec #MassSpectrometryhttps://www.matrixscience.com/help/protein_inference.html
- Mascot Newsletter, October 2024: Mascot Server 3.0 is released. Thermal proteome profiling and affinity ultrafiltration for analysing natural products. Mascot at HUPO 2024. #proteomics #MassSpec #MassSpectrometryhttps://www.matrixscience.com/nl/202410/newsletter.html