- by UnknownHappy 2026 US Researchers! Here is your friendly reminder that the NIH did have time to invent not one – but 2 (two!) new required Biosketch formats. Ignore the one from the summer. Starting January 2026 your BioSketch MUST be made through the ScienV portal at the National Library of Medicine. (Yes that still exists, it's just the Goddard Library that is being shut down and the books thrown out).I assume the NIH came back from the longest shutdown in US government history as surprised by this as we are, so there is a glitch or two. If your ORCID won't connect […]
- by UnknownLink to the original post (with patent links here). I tried opening it in a browser I'm not logged in to (into…? en to? e tu? something) and it worked!
- by UnknownThis is probably a great idea, but it ABSOLUTELY has a funny parallel I haven't been able to stop thinking about.Wow. Did I ever go down a rabbit hole. I was looking for a MadTV skit and then discovered that SNL did it first – in 1976! SNL did a 3 blade razor. MadTV stepped it up a little with 20 ion funnels! razor blades! (Youtube link)
- by UnknownOkay, so even if this is possible after I've repeatedly said it probably isn't (yet?), I hope we can all look at this and agree that it looks like a complete pain in the butt to actually do. But…I don't like bulk phosphopeptide enrichment, so I have my biases. Now, you could probably argue immediately that 83 phosphopeptides from one HeLa cell isn't very much. And I'd also agree with that, but it's probably more than I could get without enrichment. Though….I might legitimately see if that is true on an Ultra2. We do have some cancer cells coming in….
- by UnknownPaper link if you don't want a history of p53 and why I love it. p53 (TP53 to some people for some reason) is super weird, it's also super important, the TP doesn't stand for the bathroom paper Americans are obsessed with for some reason. It stands for Tumor suPressor (I didn't come up with the name it was discovered by 3 or 4 researchers independently the year I was born). It's one of my favorite proteins, not only because my first postdoc was on it's very close friend p53 binding partner #1 (53BP1), but also because its the best example […]
- by UnknownI saw foxes and plasma in the title and I automatically assumed I knew one of the authors. I skimmed through and found that there were multiple seasonal proteins altered and thought that seemed pretty unlikely without extensive nanoparticle depletion. Those top proteins in blood plasma don't really seem to change all that much. Then I realized we weren't talking about blood plasma. We are talking about another plasma! Aha. That's a thing! And it's a crab eating fox! I didn't know that was a thing either! The method section is interesting, particularly the animal conditioning, but also the mass spectrometry! Proteins were […]
- by UnknownIf you were thinking "cool, maybe someone made the great iRT triggering tools for Q Exactive available again!" maybe they have, but this isn't where they did it. This group did do targeted by standard PRM assay for the host-cell proteins that they are most worried about, and I've never seen this before. Generally host cell protein analysis is done by untargeted means – at least in the literature. The figures are nice, and they focus a lot of their reproducibility and ability to port these methods to other chromatography gradients, hypothetically. They also back up some of their results by […]
- by UnknownHow would your favorite mass spec do in a head to head in a process control pipeline? What's that? Something practical in manufacturing things, probably! Ask an AI, it will make some dumb shit up about it. Google's moron of an AI I can't seem to turn off just lied to me extensively about how many points different field goals in basketball count for. Unless I started the New Year with a stroke, I'm pretty sure I can't trust Doogle for anything now and I'm happy about this surprisingly inexpensive IOS migration in progress. What, was I typing about a paper? […]
- by UnknownI am making this post to boost the Altmetric score of my accepted paper. Sorry, it got desk rejected from the target journal and ended up in one I'd never heard of and I want ALL THE ACCESSES. But…if you want to see my paper with the editorial glitch by the journal which inserted figures from a completely unrelated paper about COVID, check this out. It's legitimately weird. Thanks to Dr. Slavov for pointing it out. For an actual blogpost about these papers check out this weird Springer blog. https://communities.springernature.com/posts/single-cell-histone-modifications-can-be-readily-quantified-in-single-cell-proteomic-datasetsIf you want to see the targeted one where they derivatized the lysines(!!) with robots, check […]
- by UnknownI kept forgetting this was a thing. Apparently, I even met some of the authors, but I was with the Director of Mass Spectrometry Innovation at some fancy place in California, and since we only hang out at conferences we tend to end up out until morning and I may not always form long-term memories at conferences with mass spec nerds everywhere. Which is funny when I pitch a collaboration and I'm all like "nice to meet you" and they are like "…ummm….what……" This is the original paper! This is the big picture! (Click to expand, or go to the paper link […]
- by UnknownThanks to our colleagues and affiliation with the Pittsburgh Liver Research Center we get access to a lot of human liver samples. Typically, however, this is because the liver is taken out of a person so that a new one can be put in. They don't typically take those livers out because they're in great shape. They're generally super extreme cases of bad livers. As you dig through the repositories you'll find that is often the case. Not a ton of helathy controls of livers or less terminal liver disease samples. Merry Xmas to me! Seven healthy controls WITH both liver homogenate […]
- by UnknownAntibody and antibody drug conjugate (ADC) drugs are EVERYWHERE. You can't watch 3 minutes of YouTube or Television (is that still a thing? I can't believe YouTube still exists at all either, how hard would it be to replace it with something that was good?) Do you have to run 1 hour or 2 hour gradients of digests to work them out? If so, you sure couldn't keep up with a big multi-lot multi-batch generation facility.Could you just digest and direct infuse? Probably! But how would you analyze those data? With iFishMass! They start off by doing some antibodies and ADCs with […]
- by UnknownWHOA! Okay, I am just dropping this here so I can look at it later and see if it's helpful for us because I don't currently have a way to easily do this with protein data at all! (Translation: I haven't read this, but I'll see it and remember to later if it is here!)
- by UnknownI pitched mass spec based spatial proteomics a while back at a building where they have a whole ton of microscopes that are cooled with cryogens. Supposedly someone in the audience invented the whole idea of making a microscope super cold. A Wikipedia suggests that isn't all that unlikely. Right zipcode for sure. When they started asking questions about spatial resolution and I enthusiastically gave the best I'd ever seen, it sucked all the interest out of the entire room. For real, I think they considered not validating my parking. The microscopy people can't look at a lot of proteins at […]
- by Unknown5 categories! 5 prestigous awards from probably the coolest proteomics groups on the planet! You can nominate and find the rules and all the important stuff here!
- by UnknownWell…this one looks like magic…I was reading it on my phone yesterday, but I'm reasonably sure this was a custom Nano DESI source equipped on an Orbitrap Fusion 3 (Eclipse) or 4 (Assend). There are some ridiculously nice pictures in it, but if you're getting spatial localization of large proteins or medium sized protein complexes, there is some impressive mass spec wizardry going on here. Localizing a 185kDa protein in a human kidney??? Whoa. One of two really impressive spatial proteomics papers that dropped this weekend. The other one is in my wheelhouse a bit more and I'll probably get to […]
- by UnknownThis isn't super new, but a colleague sent it to me and it's really really cool.The liver is really weird and even though from a microscopic level it looks like a bag of square (they like "cuboidal") cells all stacked like bricks row after row forever, these cells are very different depending on where they are. These big bricks of cells are also packed full of mitochondria and may have hundreds of them per cell. This group used spatial sorting to get piles of hepatocytes from different zones THEN did mitochondrial enrichment THEN did (TMT) proteomics and phosphoproteomics.There are big […]
- by Unknown1) Today I just discovered the ACS Journal of Chemistry Education (or maybe re-remembered it was a thing?) And it's a treasure trove of interesting stuff. Example? Check out this cool story from the pandemic where students remotely analyzed DIA generated MS2 spectra. This is how it went (stolen brazenly from the paper)!
- by UnknownI can't possibly spend the time on this new study that it deserves, but I really am going to think about it on my commute today. Or listen to the Halo Effect album I didn't know about until yesterday because that's how busy my 2025 has been. And maybe also think about this paper.When Anna Barker was on THE Proteomics Show podcast she stressed how absolutely critical the sample handling was to the setup of CPTAC (that was pretty much her idea, btw). I asked her what she thought about all the people who are just pulling from repositories and […]
- by UnknownI'd have went with the MCP abstract graphic but it's all smooshed up on my screen.Related, we just had Mike MacCoss on The Proteomics Show (dude won the Don Hunt award for distinguished contribution in proteomics!). I try to always ask guests what they're most excited about in the present/future of proteomics and he cited clinical and translational assays that have happened or are happening. FINALLY! (I added that bit, 'cause it's about time!) Having trouble coming up with a list of them? Here's one to add! Check out this sick new one I just stumbled onto while not at all […]
Mass spectrometry websites, social media, journal feeds, and other links and items of interest to the mass spectrometry community.
