RSS feed of mass spectrometry journals and other mass spectrometry articles and social feeds.
- Mass spectrometry (MS)-based phosphoproteomics has transformed our understanding of cell signaling, yet current workflows face limitations in sensitivity and spatial resolution when applied to sub-microgram scale protein inputs. Here, we present nanoPhos, a robust method for ultra-sensitive phosphoproteomics, which allows deep coverage at high throughput and is compatible with Deep Visual Proteomics (DVP). It employs loss-less solid phase extraction capture […]
- Structural elucidation using untargeted tandem mass spectrometry (MS/MS) has played a critical role in advancing scientific discovery. However, differentiating molecular fragmentation patterns between isobaric structures remains a prominent challenge in metabolomics, drug discovery, and reaction screening, presenting a significant barrier to the cost-effective and rapid identification of unknown molecular structures. Here, we present a geometric deep learning model, ICEBERG, that […]
- Just stealing Chris's post! Bonus, if you see Ian watch what he's eating and model your diet after it. He's at least 114 and he's out there on stage giving talks at 8 in the morning? Oh! Website went up on the Astral 2 "Zoomie"270 Hz and the resolution to do TMT 32-plex (I thought it was 35? Does anyone know?) Big […]
- Mass spectrometry instrumentation continues to evolve rapidly, yet quantifying these advances beyond conventional peptide and protein detections remains challenging. Here, we evaluate a modified Orbitrap Astral Zoom mass spectrometer (MS) prototype and compare its performance to the standard Orbitrap Astral MS. Across a range of acquisition methods and sample inputs, the prototype instrument outperformed the standard Orbitrap Astral MS in […]
- Chordoid glioma (ChG) is a rare, low-grade brain tumor characterized by a novel recurrent point mutation, D463H, in the kinase domain of protein kinase C alpha (PKC). The mutation is invariably an Asp to His substitution, suggesting it endows a unique function beyond catalytic inactivation associated with other cancer-associated PKC mutations. Here we use in vitro and in cellulo activity […]
- If you've seen me talk about single cell proteomics, I like to point out the proteins I can see (50,000 copies and up) and I make a joke suggesting you come back in a decade if you're interested in transcription factors.They turn on transcription at your DNA…you only have a couple copies of each gene. In many, most cases, we […]
- Read more here! It sure makes the Illumina protein prep 384 samples/week seem even sillier…..
- Hardware is already leaking to the media? Here is the first one – and a surprisingly well-written article with insight from a bunch of early adopters and/or beta testers. Oh crap, and my news feed was like "oh, so you like mass spectrometers? First I've ever heard you mention that… here's another! Looks like it's a top-down focused instrument with MSn(?) capabilities […]
- J. Anal. At. Spectrom., 2025, Accepted ManuscriptDOI: 10.1039/D5JA00108K, Technical Note Open Access   This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.Raven L. Buckman Johnson, Hark Karkee, Alexander Gundlach-GrahamSingle-particle inductively coupled plasma time-of-flight mass spectrometry (spICP-TOFMS) can be used to measure metal-containing nanoparticles (NPs) and sub-micron particles (µPs) at environmentally relevant concentrations. Multielement fingerprints measured by spICP-TOFMS […]
- Publication date: Available online 30 May 2025Source: Journal of ProteomicsAuthor(s): Sandrine Reymond, Lyssia Gruaz, Domitille Schvartz, Anna Penalba, Joan Montaner, Jean-Charles Sanchez
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00029
- ABSTRACT Liquid chromatography-mass spectrometry (LC–MS) is a powerful technique for the detection and quantification of methylated quaternary ammonium compounds (mQACs), such as acylcarnitines and methylated amino-acid-derived (betainized) compounds, in biological matrices. Due to their high polarity and permanent charge, mQACs present analytical challenges, particularly in achieving efficient chromatographic retention and resolution. Here, we focus on the application of hydrophilic interaction […]
- ALK and ROS1 fusions are emerging as key drivers of infant-type hemispheric gliomas (IHG). With diverse gene partners, the impact of ALK and ROS1 oncoprotein heterogeneity on glioma biology remains unknown. We developed an integrative phospho-proteomic and transcriptomic approach to discover biological functions regulated by five IHG-associated fusions: CCDC88A::ALK, PPP1CB::ALK, GOPC::ROS1, CLIP1::ROS1 and KIF21A::ROS1. Here, we report fusion-specific oncogenic functions […]
- ABSTRACT Rationale In this study, the chemical structure of a unique lipid A derived from the phenol-phase soluble lipopolysaccharide (LPS) of Vibrio anguillarum serovar SJ-41, a virulent marine and freshwater pathogen, was investigated using electrospray ionization with field asymmetric wave ion mobility-Orbitrap mass spectrometry (ESI-FAIMS-MS). The analysis indicated that the obtained lipid A consisted of a heterogeneous mixture of molecules. […]
- ABSTRACT Rationale Bletilla striata (Thunb.) Rchb.f. (Orchidaceae), a traditional Chinese medicine herb known as baiji, exhibits diverse pharmacological activities. Despite extensive research on its phytochemistry, the volatile organic compounds (VOCs) of B. striata remain largely unexplored. Methods Gas chromatography-ion mobility spectrometry (GC-IMS) was used to analyze and compare the VOCs of three pre-processing methods (fresh, boiling, and steaming) and four […]
- ABSTRACT Rationale Clinically, cyclosporine (CsA) plays a crucial role in the treatment of aplastic anemia (AA) and has demonstrated significant therapeutic efficacy. We applied DIA-based quantitative proteomics to analyze plasma protein profiles in AA patients, aiming to identify proteins and pathways modulated by CsA, thereby elucidating its therapeutic mechanisms. Methods Plasma samples from three AA patients pre– and post–CsA treatment […]
- Journal of Mass Spectrometry, Volume 60, Issue 6, June 2025.
- Cellular differentiation processes are largely orchestrated by variation in transcription factor (TF) abundance. Since these proteins are usually expressed at extremely low levels, studying TF-driven cellular processes using single-cell proteomics by mass spectrometry (scp-MS) has been challenging. Here we present informed DIA (iDIA), an acquisition method tailored towards low-input and scp-MS. iDIA combines targeted and global proteomics in a single […]
- Resistance of chronic myeloid leukemia (CML) to BCR-ABL tyrosine kinase inhibitors (TKIs) can arise from kinase-activating mutations within the ABL kinase domain, BCR-ABL gene amplification, or mechanisms independent of BCR-ABL. Through quantitative mass spectrometry analysis of K562, a commonly used CML cell line, and ImR, an imatinib-resistant derivative we developed, global changes in the proteome and phosphoproteome were identified. LIN28A, […]
- Protein degradation is critical for regulating cellular functions. Therefore, there is considerable interest in developing effective proteome-wide strategies to identify protease cleavage products to enhance our understanding of proteolytic pathways and their perturbation in diseases. Here, we present a streamlined N-termini proteome analysis leveraging N-Hydroxysuccinimide (NHS) ester chemistry. At the protein level, N-terminal amines (naturally occurring protein N-termini, lysines, or […]
- Argpyrimidine (APY) is a methylglyoxal-derived advanced glycation end-product (AGE) that has been associated with multiple diseases. As APY formation occurs without an enzyme, it remains exceptionally difficult to pinpoint where APY is likely to be found, both on individual proteins and in cells. In this study, we used a peptide model system and mass spectrometry analysis to investigate the chemical […]
- Hypoxia inducible factors (HIFs) are transcription factors that coordinate cellular responses to low oxygen levels, functioning as an /{beta} heterodimer which binds a short hypoxia response element (HRE) DNA sequence. Prior studies suggest HIF/HRE complexes are augmented by the binding of additional factors nearby, but those interactions are not well understood. Here, we integrated structural and biochemical approaches to investigate […]
- The threat and incidence of fungal diseases are increasing, as is the severity and mortality rates associated with these infections. New strategies to combat fungal infections are urgently needed to overcome rising rates of resistance and the emergence of new pathogens. To promote invasion within a host, fungi use highly adapted and regulated virulence factors, and, in turn, the host […]
- Post-translational modification of proteins by polyamines (putrescine, spermine and spermidine) is poorly studied due to technical challenges in identification. Polyamination is mainly performed by transglutaminases via transamidation of a polyamine to an acceptor glutamine-residue on the target protein. We performed polyamination reactions on whole cell lysates using 2 different polyamine probes (Biotin-pentylamine and DNP-pentylamine) and identified modified peptides by mass […]
- J. Anal. At. Spectrom., 2025, Accepted ManuscriptDOI: 10.1039/D5JA00142K, PaperYixuan Li, Haoyu Shi, Peng Wang, Yuxiang MoWe present a novel method for precision measurement of 207Pb/206Pb and 208Pb/206Pb isotope ratios in solid samples, utilizing vacuum ultraviolet (VUV) laser ablation/ionization coupled with a time-of-flight reflectron mass spectrometer…The content of this RSS Feed (c) The Royal Society of Chemistry