RSS feed of mass spectrometry journals and other mass spectrometry articles and social feeds.
- Palytoxin (PLTX) and its analogues from Ostreopsis cf. ovata are significant health concerns. They show potent vasoconstrictive properties, often causing seafood poisoning. PLTX analogues have chiral centers, resulting in many isomers, making their separation by liquid chromatography and identification/characterization by mass spectrometry challenging. This study explores for the first time the ion mobility spectrometry (IMS) behavior of these compounds to […]
- Background: High-throughput affinity and mass-spectrometry-based proteomic studies of large clinical cohorts generate high-dimensional proteomic data useful for accelerated disease biomarker discovery. A powerful approach to realizing the potential of these big, complex, and non-linear data, whilst ensuring reproducible results, is to use automated machine learning (ML) and deep learning (DL) pipelines for their analysis. However, there remains a gap in […]
- Mascot Daemon can export protein level quant intensities for replicate LFQ, SILAC and iTRAQ/TMT. The latest version can also export intensity values calculated using the "Top-3" method, illustrated here with DDA-PASEF data.https://www.matrixscience.com/blog/top-3-quantitation-summary-in-mascot-daemon-3-using-dda-pasef.html#proteomics #massspec
- Publication date: Available online 17 October 2025Source: Journal of ProteomicsAuthor(s): Jhenifer Yonara de Lima, Emanuella de Castro Andreassa, Marlon Dias Mariano Santos, Paulo Costa Carvalho, Tatiana de Arruda Campos Brasil de Souza
- ABSTRACT Understanding kidney metabolic heterogeneity is critical for unraveling diabetic nephropathy (DN) pathogenesis. While Huangqi Guizhi Wuwu decoction (HGD) shows efficacy against DN, its spatial metabolic effects remain unknown. We integrated liquid chromatography–mass spectrometry (LC–MS) metabolomics, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), and tandem mass tag (TMT) proteomics to map systemic and microregional metabolic landscapes in HGD-treated db/db mice. […]
- pUL21 is a conserved and multifunctional alphaherpesvirus tegument protein that is critical for both early and late stages in the herpes simplex virus type 2 (HSV-2) replication cycle; however, how pUL21 participates in these activities is poorly understood. To help elucidate the role of pUL21 in these various activities, we used a proximity-dependent biotin identification (BioID) approach by constructing an […]
- Crosslinking mass spectrometry (XL-MS) could replace traditional techniques for sampling the cellular interactome, such as affinity pulldown MS. It generates superior interaction data that can be used to better model cellular structure and function. However, the sampling depth of current XL-MS techniques is disappointing. Poor sampling is often blamed on a low-yielding crosslinking reaction, estimated to be ~0.1% based on […]
- Ruminant animals are a major source of the potent greenhouse gas methane, but they are also a tractable target for climate solutions. Several strategies have been developed to lower methane emissions from ruminants, including feed additives that inhibit methanogenic archaea. Sustainable solutions must eliminate methane emissions without hampering the microbial fermentation of plant material, which the animal host relies on […]
- The de novo design and assembly of a DNA genome represents an important milestone toward the construction of a minimal synthetic cell. The genome must contain all the instructions to enable primary cellular functions, probably comprising over 150 genes with a total size over 200 kb. Here, we designed and built a partial synthetic genome that satisfies the requirements for […]
- Volatile organic compounds (VOCs) in urine have shown increasing promise as non-invasive biomarkers for the early detection and monitoring of diseases. However, their volatile nature may make them highly susceptible to pre-analytical factors, presenting significant challenges for consistent biomarker discovery and reproducibility within metabolomic workflows. This study aimed to assess the impact of four critical pre-analytical variables, including fasting state, […]
- Publication date: Available online 15 October 2025Source: Journal of ProteomicsAuthor(s): Hiba Salim, Ruben Almey, Laura Pont, Fernando Benavente, Maarten Dhaenens, Estela Giménez
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00251
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00331
- Motivation: Hydrogen-deuterium exchange/mass spectrometry (HX/MS) is a rapidly expanding technique used to investigate protein conformational ensembles. The growing popularity and utility of HX/MS has driven the development of diverse instrumentation and software, resulting in inconsistent, non-standardized data analysis and representation. Most HX/MS data formats also employ only centroid-level representations of the data rather than full isotopic mass spectra, reducing the […]
- PROTEOMICS, EarlyView.
- PROTEOMICS, EarlyView.
- ABSTRACT Rationale Light stable isotopes (δ 13C, δ 15N, δ 2H, and δ 18O) of Ectropis grisescens (Lepidoptera: Geometridae), a destructive tea leaf-eating pest, were measured at different developmental stages. Isotope values of larval instars, pupae, and adult moth body tissues were determined to understand fractionation patterns at different life stages and to evaluate dietary isotopic change from food to […]
- Mass spectrometry imaging (MSI) is a powerful tool in both basic and clinical research, enabling spatial visualization of biomolecules and drugs in tissue sections. However, factors influencing mass spectrometric response during imaging are increasingly recognized for their impact on apparent molecular distribution. Quantitative mass spectrometry imaging (qMSI) addresses this variability by incorporating analytical standards that undergo the same processes as […]
- Tauopathies are a group of neurodegenerative disorders, including Alzheimer disease, that are neuropathologically defined by deposition of pathological forms of tau in the brain. While tau is reported to drive neurotoxicity by negatively affecting cytoskeletal, nucleoskeletal, and genomic architecture, the mechanisms mediating tau-induced dysfunction of the cytoskeleton and nucleoskeleton are incompletely understood. Based on proteomic profiling, we identify a suite […]
- Ubiquilin-2 (UBQLN2) is a ubiquitin (Ub)-binding shuttle protein that is mutated in X-linked forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). ALS/FTD-linked mutations in UBQLN2 disrupt its conformation, increasing its tendency to form cytoplasmic aggregates that may disrupt cellular regulation through loss-of-function (LOF) and gain-of-function (GOF) effects. To explore how ALS-associated mutations impact UBQLN2 function, we performed quantitative […]
- The identification of bacteria is central to the microbiological sciences. While gene sequencing methods have been the standard to identify bacteria, use of MALDI-TOF mass spectrometry (MS) in clinical microbiology provides high-throughput identification to the subspecies level. However, biotyping has yet to be adopted outside of clinical settings due to the lack of a centralized public database of MS protein […]
- Anal. Methods, 2025, Advance ArticleDOI: 10.1039/D5AY01057H, Paper Open Access   This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.Fiona Regan, Catherine Allen, Jenny LawlerA sensitive contaminantion-controled LC-MS/MS workflow quantifies 11 phthalate diesters in environmental samples with sub ng L−1 limits.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of […]
- N-glycosylation is a post-translational modification of proteins that represents a critical quality attribute (CQA) for therapeutics like monoclonal antibodies (mAbs), directly affecting drug efficacy, safety, and stability. Real-time CQA monitoring analytical platforms depend on rapid N-glycan release and fluorophore labeling chemistries to support automated bioprocess analytics during mAb manufacturing. Procainamide is a well-known fluorophore used for released N-glycans reducing sugar […]
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c04349
- [ASAP] A Universal Spectrum Annotator for Complex Peptidoforms in Mass Spectrometry-Based ProteomicsAnalytical ChemistryDOI: 10.1021/acs.analchem.5c02832