Combined RSS Items
- Nuclear pore proteins (Nups) in yeast, flies and mammals physically interact with hundreds or thousands of chromosomal sites, which impacts transcriptional regulation. In budding yeast, transcription factors mediate interaction of Nups with enhancers of highly active genes. To define the molecular basis of this mechanism, we exploited a separation-of-function mutation in the Gcn4 transcription factor that blocks its interaction with the nuclear pore complex (NPC) without altering its DNA binding or activation domains. SILAC mass spectrometry revealed that this mutation […]
- Journal of Mass Spectrometry, Volume 59, Issue 6, June 2024.
- Journal of Mass Spectrometry, Volume 59, Issue 6, June 2024.
- A Broadened Class of DonorâAcceptor Stacked Macrometallacyclic Adducts of Different Coinage MetalsChemistry â A European Journal, Accepted Article.
- There are all sorts of thing out there now that can learn from millions (billions?) of MS/MS spectra and can take your sequence and predict how it will fragment.Which one is best? Who knows? Maybe we should have an algorithm DEATH MATCH! Okay, again I'm scrambling to wrap up things before they take my keys and kick me out on the street, so I'm leaving this here mostly for you (well…me…maybe…cause I do want to be able to find this later).HOWEVER […]
- The human dual specificity tyrosine phosphorylation regulated kinase 1A (DYRK1A) is implicated in the pathology of Down syndrome, microcephaly, and cancer, however the exact mechanism through which it functions is unknown. Here, we have studied the role of the Drosophila ortholog of DYRK1A, Minibrain (Mnb), in brain development. The neuroblasts (neural stem cells) that eventually give rise to differentiated neurons in the adult brain are formed from a specialized tissue in the larval optic lobe called the neuroepithelium, in a […]
- The clownfish – sea anemone system is a great example of symbiotic mutualism where host 'toxicity' does not impact its symbiont partner, although the underlying protection mechanism remains unclear. The regulation of nematocyst discharge in cnidarians involves N-acetylated sugars like sialic acid, that bind chemoreceptors on the tentacles of sea anemones, leading to the release of stings. It has been suggested that clownfish could be deprived of sialic acid on their skin surface, sparing them from being stung and facilitating […]
- Publication date: Available online 11 May 2024Source: Journal of Chromatography AAuthor(s): Suo Decheng, Song Zhanteng, Xiao Zhiming, Li Yang, Liu Wuyan, Wang Junming, Fan Xia
- Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.3c09347
- PROTEOMICS, EarlyView.
- Journal of Mass Spectrometry, Volume 59, Issue 6, June 2024.
- Journal of Mass Spectrometry, Volume 59, Issue 6, June 2024.
- Journal of Mass Spectrometry, Volume 59, Issue 6, June 2024.
- Journal of Mass Spectrometry, Volume 59, Issue 6, June 2024.
- Journal of Mass Spectrometry, Volume 59, Issue 6, June 2024.
- Journal of Mass Spectrometry, Volume 59, Issue 6, June 2024.
- Shengxian decoction, a traditional Chinese medicinal prescription, has been shown to alleviate doxorubicin-induced chronic heart failure. This study established an ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method to separate and characterize the complex chemical compositions of Shengxian decoction, and the absorbed compounds in the bio-samples of the cardiotoxicity rats with chronic heart failure after its oral delivery. Note that 116 chemical compounds were identified from Shengxian decoction in vitro, 81 more than previously detected. Based on the three-dimensional data of […]
- Bone resorption involves dissolution of mineral and enzymatic degradation of bone matrix. The primary enzyme is cathepsin K but other proteases including matrix metalloproteinases are involved. Some cleavage products of cathepsin K have been partially identified, including crossed-linked telopeptides of type I collagen. However, the pathway of type I bone collagen degradation has not been fully elucidated. The aim of this study was to comprehensively characterise the entire complement of bone breakdown products resulting from osteoclast action under controlled conditions […]
- The plasma proteome is maintained by the influx and efflux of proteins from surrounding organs and cells. To quantify the extent different organs and cells contribute to the plasma proteome composition, we developed a mass spectrometry-based proteomics strategy to infer the origin of proteins detected in human plasma in health and disease. First, we constructed an extensive human proteome atlas from 18 vascularized organs and the most abundant cell types in blood. Second, the atlas was interfaced with previous RNA/protein […]
- Among neurons, retinal ganglion cells (RGCs) are uniquely sensitive to mitochondrial dysfunction. The RGC is highly polarized, with a somatodendritic compartment in the inner retina and an axonal compartment projecting to targets in the brain. The drastically dissimilar functions of these compartments implies that mitochondria face different bioenergetic and other physiological demands. We hypothesized that compartmental differences in mitochondrial biology would be reflected by disparities in mitochondrial protein composition. Here, we describe a protocol to isolate intact mitochondria separately from […]
- Analytical ChemistryDOI: 10.1021/acs.analchem.4c01119
- Thanks r/massspectrometry! I didn't know about this. I have Gary's Mass Spectrometry for Biotechnology which was a key part of Iulia Lazar's class on bioanalytical instrumentation when I was in grad school. This is on an obviously inferior -omics but I bet it's a solid read and it's like $14 in paperback!Â
- Abstract A multimodal approach combining inductively coupled plasma mass spectrometry (ICP-MS), single-particle ICP-MS (spICP-MS), scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDS) and Raman spectroscopy enabled a deeper insight into the balance between total titanium (Ti), the soluble titanium fraction and titanium dioxide based particle fraction levels in periprosthetic tissues collected from patients undergoing revision surgery. Hydrofluoric acid usage in the sample digestion allowed for complete digestion of TiO2 particles, thus enabling accurate estimation of total Ti levels. The TiO2 fraction represents 38â94% […]
- Publication date: 15 August 2024Source: Talanta, Volume 276Author(s): Eduardo Bolea-Fernandez, Ana Rua-Ibarz, Jorge Alves Anjos, Frank Vanhaecke
- Â If you've never done it, it absolutely sucks to kill a lab animal to get samples. Not only is it awful but an increasing body of evidence suggests that those millions of years or evolution make it not make very much sense at all to do it for many mechanisms. However, there are some systems where you absolutely have to do it to get that information. What if instead of needing to murder dozens of animals you could learn everything […]