Combined RSS Items
- Journal of Mass Spectrometry, Volume 61, Issue 5, May 2026.
- Publication date: Available online 14 April 2026Source: International Journal of Mass SpectrometryAuthor(s): Anushree Dutta, Pintu Mandal, Nabanita Deb
- Publication date: Available online 14 April 2026Source: Journal of Chromatography AAuthor(s): Ebrahim Azadniya, Kathrin Billerbeck, Gertrud E. Morlock
- Publication date: Available online 14 April 2026Source: Journal of Chromatography AAuthor(s): Azemina Bajramova, Evangelos Synefakis, Jesper Larsen, Jeppe Lindegaard Hjorth, Charlotta Turner, Margareta Sandahl
- For the first time in a long time, I had to do some EvoSep troubleshooting. Turns out that ceramic needle thing can get clogged! Gabriel at EvoSep led me to this super useful online resource that walks you through step by step to get it all worked out. It's amazingly clear with pictures and "did it work? click here!" AND 4 MILLION PERCENT BETTER than letting Adobe's class trailing LLM help you dig through the user manual. If you see a button […]
- Pantothenamides (PanAms) comprise a promising class of antimalarial compounds that kill asexual blood-stage Plasmodium falciparum parasites and block transmission. Intriguingly, the most advanced PanAm in drug development, MMV693183, is approximately 100 times more potent against female gametocytes than males. We hypothesized that this specificity is explained by a difference in PanAm uptake, which we studied using a PanAm-based photoaffinity labelling (PAL) probe. We successfully synthesized a probe that competed with MMV693183 in drug sensitivity assays, while the probe did not […]
- Okay, we have to take a look at this for real. I do like the color scheme on these plots, though…As an aside, I ran a commercial program for some people recently and it gave me 20% more protein groups than the ones I currently use. Those extra 20% really annoyed my collaborators. They were …like… biologically very very unlikely…? Not DIA-NN, a commercial thing, but I did re-learn a lesson that more peptides isn't always a better. But DIA-NN […]
- Wow…. I do just have to leave this here and move on. I've already forwarded the paper to a bunch of people, though, and can't wait to spend more time on it. We need to figure out how much DDM you can use before it's a bad thing, though! This group used 6-8x more than what we use, and they get a lot more membrane proteins…..Totally worth taking a look at!
- Single-cell proteomics (SCP) has emerged as a powerful approach to quantify protein expression variability at cellular resolution, yet most state-of-the-art workflows are tailored to eukaryotic cells with only one study exploring how single bacteria can be analyzed by mass spectrometry. Here, we established bacSCP, a protocol extending SCP to bacterial cells, facing analytical challenges such as the thick bacterial cell wall hampering lysis, the extremely small cell size and resultant low protein content, and the consequently relatively high level of […]
- Spatial multi-omics methods allow researchers to study complex biological systems by integrating multiple molecular layers while preserving their spatial organization. However, integrating spatial transcriptomics and mass spectrometry imaging (MSI) remains challenging due to the differences between the two modalities, including sampling geometry, spatial resolution, signal scaling, and measurement principles. For example, 10x Genomics Visium captures transcriptomic data on a discrete hexagonal grid of spots; however, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) produces dense Cartesian pixel maps of molecular distributions. […]
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c04968
- Sweroside is an important iridoid compound which exhibits various pharmacological and biological activities. The intracorporeal metabolic fate of sweroside was elucidated via the UHPLC-ESI-MS method combined with picolinoyl derivatization. Sweroside can be metabolized in vivo to swerosimetabolin A and new metabolite swerosimetabolin B after gavage administration. ABSTRACT Sweroside (SRD) is an important iridoid compound that exhibits various pharmacological and biological activities. However, it has been suggested that the remarkable pharmacological activity is due to the metabolites of the iridoids rather […]
- ABSTRACT Black ginseng, produced through the traditional nine-steaming–nine-drying process, exhibits enhanced pharmacological activity compared with unprocessed ginseng. This study investigated the dynamic changes in ginsenoside composition and antioxidant activity during steam-drying using a non-targeted metabolomics approach based on ultra-high-performance liquid chromatography-mass spectrometry. A total of 73 ginsenosides were identified, among which 47 differential markers were screened by principal component analysis and partial least squares discriminant analysis. The relative quantification of 29 key markers revealed distinct transformation patterns of ginsenosides during […]
- Publication date: Available online 12 April 2026Source: TalantaAuthor(s): Yi Pan, Keon Ho, Sunwoo Kim, Jisik Jung, Dengfeng Sun, JieBin Yang, Zhongjun Zhao, Jinsang Jung, Sanghyub Oh
- ABSTRACT Rationale Effective treatments for diabetic kidney disease are currently lacking. This study aimed to investigate the therapeutic mechanism of Gynura procumbens (Lour.) Merr against diabetic kidney disease. Methods The 70% ethanol extract of Gynura procumbens (Lour.) Merr was analyzed by UPLC-Q/TOF-MS/MS. A diabetic kidney disease mouse model was established to evaluate its efficacy. Mechanisms were predicted via network pharmacology and validated through transcriptomic, protein, and gene expression analyses. Results A total of 32 compounds and 13 active ingredients were […]
- A Novel Approach for Rapid Screening of Stilbenes in Plant Extracts Based on SALDI Mass SpectrometryABSTRACT Rationale Stilbenes are a class of plant phenolic secondary metabolites with a wide range of biological activity and pronounced antioxidant properties, which are important for various industries. This determines the urgency of developing new methods for identifying stilbenes in new plant sources. An original approach solving this challenge is proposed in this article. Methods Three standard samples of stilbenes rhaponiticin, isorhapontigenin, and polydatin were used to optimize the experimental conditions. Three plant extracts used for approbation were obtained by […]
- Microtubules (MTs) play central roles in the organization and morphology of trypanosomatid parasites, forming highly specialized cytoskeletal structures such as the subpellicular corset, the flagellar axoneme, and the mitotic spindle. Functional specialization of MTs is regulated by the "tubulin code", which is defined by the combination of different – and {beta}-tubulin isotypes, a set of post-translational modifications (PTMs) and specific MT-binding proteins. Although multiple tubulin PTMs have been described in trypanosomatids using specific antibodies or mass spectrometry, to date no […]
- Molecular studies of brain receptors and transporters have typically relied on recombinant systems, limiting insight into their organization in native tissue. Here, we develop nanobody-based immunoprecipitation coupled with native mass spectrometry to interrogate endogenous protein assemblies from post-mortem mouse and human brain sections. We exemplify our approach by characterizing the synaptic proteins VGluT1 and mGluR2. From a single mouse brain, we discover mGluR2/3 heterodimers, alongside mGluR2 homodimers. Considering regions of human brain heterodimeric mGluR2/3 is highly abundant in the OFC […]
- Publication date: Available online 11 April 2026Source: International Journal of Mass SpectrometryAuthor(s): Payten A. Harville, Thien Khuu, Nan Yang, Michael B. Rosen, Mark A. Boyer, Meng Huang, Anne B. McCoy, Mark A. Johnson
- Mass spectrometry (MS) is a powerful technique for characterizing modified RNA as it directly sequences and quantifies all mass-altering modifications simultaneously. However, the physicochemical properties of RNA result in poor ionization efficiencies during electrospray ionization, presenting a major barrier to sensitive MS measurements necessary for low abundance RNA samples and RNAs with low modification stoichiometries. Here, we report a ligation-based approach to increase ionization efficiencies of RNA oligonucleotides. We show that short (~5 nt), chemically modified DNA oligonucleotides can be […]
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.6c00045
- Publication date: Available online 9 April 2026Source: Journal of Chromatography AAuthor(s): Yuki Fukushima, Kazuhiro Yamamoto, Koichi Machida, Akira Kotani, Hideki Hakamata
- More than 600 distinct skeletal muscles constitute up to 40% of the total mass of the human body. Human skeletal muscles differ in anatomical position, morphology, origin, and function, but the diversity of their molecular phenotypes, the gene expression and protein abundance profiles, remains poorly explored. Here, we report the large-scale CAGE-Seq promoterome profiling of 75 human skeletal muscles, complemented by 22 matched proteomes obtained with mass spectrometry. We identified 37001 transcribed regulatory elements and 1804 protein groups encompassing 1895 […]
- PROTEOMICS, EarlyView.
- Alzheimer's disease (AD) is defined by progressive tau aggregation, yet the molecular events driving this process remain poorly understood. Post-translational modifications (PTMs) are key regulators of tau biology and potential biomarkers of disease progression. Using immunoprecipitation-mass spectrometry and absolute quantification of tau isoforms, we profiled tau PTMs in soluble and insoluble brain fractions. We studied multiple brain regions (hippocampus, inferior temporal and frontal gyri), representing regions affected at different stages of pathology, from human donors spanning the AD spectrum and […]