Combined RSS Items
- Publication date: March 2026Source: International Journal of Mass Spectrometry, Volume 521Author(s):
- Publication date: March 2026Source: International Journal of Mass Spectrometry, Volume 521Author(s):
- Publication date: Available online 31 January 2026Source: International Journal of Mass SpectrometryAuthor(s): Wenwu Yang, Can You, Xiaofei Qiu, Xirun Tong, Shansong Lu, Guiling Duan, Yingxiong Cai, Hanyu Deng, Hongmei Yang
- Publication date: 1 June 2026Source: Talanta, Volume 303Author(s): Yihe Liu, Jianzheng Yang, Jianying Zhang, Yan Zhou, Yichuan Tang, Tao Zhou
- Publication date: Available online 31 January 2026Source: Journal of Chromatography BAuthor(s): Ting Zou, Xiangchang He, Zhiying Yuan, Weihe He, Jie Dai, Siyu Li, Guangming Xu
- YES! Okay, so this is may finally be the smart solution to something we tried (and probably just about everyone else) with the SCoPE-MS/ScoPE2 workflows.If you have a "carrier" "boost" "basil" or "oregonO" channel, why couldn't you load that thing with phospho-enriched samples (for example) instead of 200 cells or a a diluted perfectly digested pooled sample? The reason appears to be that your coisolated peptide (or junk) background ends up leading to a preposterous number of false discoveries. Remember […]
- Publication date: 15 March 2026Source: Journal of Proteomics, Volume 325Author(s):
- Publication date: Available online 31 January 2026Source: Journal of ProteomicsAuthor(s): Xiang Li, Zhiqiong Mao, Peichan Chao, Yajin Liao, Yalan Li
- Capillary zone electrophoresis (CZE)-mass spectrometry (MS) has been proposed as a powerful analytical tool for bottom-up, top-down, and native proteomics (multi-level proteomics) decades ago to analyze complex biological samples at the levels of peptides (bottom-up), proteoforms (top-down), and complexoforms (native). However, its broad adoption has been impeded by the limited robustness and reproducibility. Here, we present multi-level proteomics data from nearly 170 CZE-MS runs (~170 hours of instrument time), demonstrating qualitatively (i.e., the number of identified peptides and proteoforms, the […]
- Stable carbon and nitrogen isotope ratios are widely used in the life sciences to investigate diet, trophic interactions, and metabolic fluxes, but conventional isotope ratio mass spectrometry requires milligram scale samples, limiting its applicability to small or rare biological specimens. Fourier Transform Isotopic Ratio Mass Spectrometry (FT IsoR MS) enables amino acid resolved isotope analysis in a proteomics compatible workflow and has previously been demonstrated at the microgram scale. Here, we assess the lower sample limit of FT IsoR MS […]
- A recent genome mining study identified class II lanthipeptides encoded in Nostoc punctiforme PCC73102 that contain acyl groups conjugated to Lys side chains. The structure and bioactivity of these peptides, named nostolysamides, were not determined. In this study, we heterologously produced the nostolysamides by co-expression of the NpuA precursor peptide with an N-terminal SUMO tag with the class II lanthipeptide synthetase NpuM in Escherichia coli. We structurally characterized the NpuA-derived product and established the position of the thioether crosslinks. All […]
- Base excision repair (BER) is the primary pathway that removes oxidatively-induced DNA base damage from the nuclear and mitochondrial genomes, with 8-oxoguanine DNA glycosylase (OGG1) initiating repair at the two most frequently-formed base lesions: 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxoGua) and 2,6-diamino-4-oxo-5-formamidopyrimidine (FapyGua). Humans expressing a catalytically-compromised variant of OGG1 (S326C) are at increased risk for type 2 diabetes, Alzheimers disease, and Parkinsons disease. To potentially enhance the overall catalytic efficiency of this variant, a prior medicinal chemistry screen discovered seven chemically distinct agonists […]
- Supercharging-enhanced nDIA-MS enables global profiling of drug-induced proteome solubility shifts
- Bio-based oxalic acid production in Issatchenkia orientalis enables sustainable rare earth recovery
- Amyloid-beta (Aβ) oligomers are key contributors to the pathology and progression of Alzheimer’s disease (AD), making their characterization essential for understanding aggregation processes and developing potential therapeutic strategies. This study provides a systematic framework for analyzing Aβ(1–42) oligomers in vitro using cyclic ion mobility-mass spectrometry (cIMS). Compared to previous generations of traveling wave ion mobility (TWIM) devices, the cIMS platform offers superior resolution through its scalable ion mobility path length. However, the multistage character of the cIMS platform requires thorough […]
- A one-pot enzymatic platform enables the biosynthesis and site-specific incorporation of chalcogen-containing tyrosine analogues (O, S, Se) into proteins in E. coli. Engineered tyrosine phenol lyase (TPL) variants and orthogonal synthetases are combined to expand the genetic code with redox-active residues, paving the way for designer proteins with tunable electronic and catalytic properties. ABSTRACT Expanding the genetic code with unnatural amino acids (UAAs) offers powerful opportunities to engineer proteins with novel redox and catalytic functions, but is often limited by […]
- This one took me a couple of rounds of putting it down and coming back to it later.It's a smart concept and a very nice thing to think about as proteomics becomes more trusted as a diagnostic. I think I first thought it was something that it isn't, and that's why I had such a conceptual problem with it. Obviously, I might still have it wrong, but this is how I'd describe it. What if you had a random patient come in […]
- I'm tired of reading today, but I really want to get back to this cool paper.Really deep multi-proteomic type analysis looking for markers for why almost everyone has liver inflammation, but for some people it's a really bad thing that progresses to worse things.You've got secretion proteomics, and neat plasma and depleted plasma, and some SomaScan data from a related study that they used, and normalized, but don't go into much. They describe the statistics and provide the output data […]
- Oh. This is really cool. I'm so glad that SCIEX is finally getting some traction with their super cool ZenoTOF hardware. The high speed high resolution mass spec world is really super competitive right now. You really can't make a bad choice (aside from Agilent, obviously – hey, I didn't tell them to abandon global proteomics instrument market -they're doing fine in their chosen niches) for getting amazing proteomics data.There is exactly one instrument out there that has big ass […]
- Stressful events are a leading factor in development of depression. The medial prefrontal cortex (mPFC) is strongly associated with depression etiology and exposure to uncontrollable stressors results in synaptic dysfunction and loss. Learned helplessness is a behavioral paradigm that measures effects of repeated exposure to uncontrollable, inescapable stress on later responses to escapable stress. We therefore performed a proteomic analysis of mPFC synaptosomes in a mouse learned helplessness model to identify molecular changes that could contribute to functional consequences of […]
- The RNA exosome is an essential and ubiquitous RNase with exonucleolytic activity, involved in ribosome biogenesis and RNA quality control in eukaryotes. It is present both in nucleus and cytoplasm, and interacts with specific cofactors in each cell compartment, which are essential for recruitment and activity control of the exosome. Posttranslational modifications are known to regulate enzyme activity and protein interaction, although their precise roles are individually specific. In this study, we investigated the phosphorylation status of proteins associated with […]
- Reactive oxygen species (ROS) are central regulators of plant growth and stress responses. Cellular ROS levels are tightly controlled by antioxidant systems, including the evolutionarily conserved catalases that detoxify hydrogen peroxide (H2O2) predominantly within peroxisomes. Despite their importance, substantial gaps remain in our understanding of catalase biogenesis, regulation, subcellular targeting, and potential extra-peroxisomal functions. Using affinity purification of the UV-B photoreceptor UVR8 coupled with mass spectrometry, we identified a REGULATOR OF CHROMATIN CONDENSATION 1–like protein in Arabidopsis, which we named […]
- Publication date: Available online 29 January 2026Source: Journal of Chromatography BAuthor(s): Christof Manuel Schönenberger, Aurelie Berthet, Matthias Briel, Alain Amstutz, Matthias Cavassini, Loïc Sartori, Camille Rime, Davide Staedler, Fiorella Lucarini
- Chemistry – A European Journal, EarlyView.
- As part of the US HUPO sponsored "Pony Express" series highlighting speakers at the upcoming 2026 US HUPO meeting in St. Louis, Ben and Ben sit down to talk with Dr. William Noble, University of Washington, recipient of the 2026 Gilbert S. Omenn Computational Proteomics Award.keywords: genomics; machine learning; AI; computational; Bridgerton; cocaine monkeys; birding