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- Background: Both the expression and activities of LINE-1 (L1) retrotransposons are known to occur in numerous cell-types and are implicated in pathobiological contexts such as aging-related inflammation, autoimmunity, and in cancers. L1s encode two proteins that are translated from bicistronic transcripts. The translation product of ORF1 (ORF1p) has been robustly detected by immunoassays and shotgun mass spectrometry (MS). Yet, more sensitive detection methods would enhance the use of ORF1p as a clinical biomarker. In contrast, until now, no direct evidence […]
- Journal of Mass Spectrometry, Volume 59, Issue 12, December 2024.
- Publication date: Available online 20 November 2024Source: Analytica Chimica ActaAuthor(s): Zhihong Wang, Christina M. Mikulski, Makayla Kent, Tod Leighfield, Gregory J. Doucette, John S. Ramsdell
- Ischemic strokes disrupt brain networks, leading to remote effects in key regions like the thalamus, a critical hub for brain functions. However, non-invasive methods to quantify these remote consequences still need to be explored. This study aimed to demonstrate that MRI-derived R2* changes can capture iron accumulation linked with inflammation secondary to stroke-induced disconnection. In order to link remote R2* changes to stroke-induced disconnection, we first conducted a secondary analysis of 156 prospectively included stroke patients who underwent MRI at […]
- Abstract Breast cancer (BC) is the leading cause of cancer death among women worldwide, making the discovery and quantification of new biomarkers essential for improving diagnostic and preventive strategies to limit dissemination and improve prognosis. Essential trace metals such as Fe, Cu, and Zn may play critical roles in the pathophysiology of both benign and malignant breast tumors. However, due to the high metabolic activity and reduced element selectivity of cancer cells, also non-essential elements may be taken up and […]
- Abstract Quality control (QC) samples are commonly used in metabolomics approaches for three main reasons: (i) the initial conditioning of the column; (ii) the correction of analytical drift especially between batches; and (iii) the evaluation of measurement precision. In practice, there are several ways to prepare and conserve QC samples. The most common in untargeted metabolomics is to pool samples after or before extraction, in order to obtain pooled QC samples accounting, respectively, for analytical variance or for both analytical […]
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00328
- ABSTRACT Methylophiopogonanone A (MOA) is one of the homoisoflavonoids isolated from Ophiopogon japonicus, which has been demonstrated to have extensive pharmacological activities. The aim of this study was to develop and validate a liquid chromatography–tandem mass spectrometry method for the measurement of MOA in rat plasma. Methylophiopogonanone B (MOB) was used as internal standard. After precipitation with acetonitrile, the samples were separated using a Waters ACQUITY HSS T3 column with 0.1% formic acid solution and acetonitrile as mobile phase. Mass […]
- Background: Obstructive sleep apnea (OSA) is characterized by intermittent hypoxia/hypercapnia (IHC), affects predominantly obese individuals, and increases atherosclerosis risk. Since we and others have implicated gut microbiota and metabolites in atherogenesis, we dissected their contributions to OSA-induced atherosclerosis. Results: Atherosclerotic lesions were compared between conventionally-reared specific pathogen free (SPF) and germ-free (GF) ApoE-/- mice following a high fat high cholesterol diet (HFHC), with and without IHC conditions. The fecal microbiota and metabolome were profiled using 16S rRNA gene amplicon sequencing […]
- Paleoproteomics sheds light on million-year-old fossils
- Insights into the nutritional value of honeybee drone larvae (Apis mellifera) through proteomic profiling
- Publication date: 1 March 2025Source: Talanta, Volume 284Author(s): Hui-I Yu, Yi-Shiou Chiou, Jie-Huei Wang, Meng-Chi Yen, Zih-Han Wu, Chia-Lung Shih
- Amino acid substitutions (AA subs) play an important role in regulating cellular activities, encompassing complex cell signaling and cycling in both normal and cancer cells. Profiling AA subs at a proteomic level has been a challenging issue in the past. In this study, we analyze a large collection of proteomic data originating from 537 patients across five cancer patient cohorts using a novel mass spectrometry data analysis tool, named PIPI3. The pervasive patterns of AA subs are present in essential […]
- Analytical ChemistryDOI: 10.1021/acs.analchem.4c04286
- Analytical ChemistryDOI: 10.1021/acs.analchem.4c01439
- Planning your conference schedule for 2025 and trying to decide which amazing US east coast city is going to get your money? US HUPO in Philadelphia? (February 22-26!) ASMS in the greatest city in the world? (June 1-5!) International HUPO in Toronto? (November 9-13!) If you're already in North America – Toronto is your one chance to get the amazing artery clogging amazingness that every European takes completely for granted. Toronto has döner kebab places EVERYWHERE! Even in NYC – which has everything – you need […]
- Study question: What are the temperature, pH and uterine fluid composition in the human uterus three days following a positive LH test or ovum pick-up? Summary answer: The mean uterine temperature was 36.94 {+/-} 0.26{degrees}C, the mean uterine pH was 6.76 {+/-} 0.22, and the concentrations of 37 components in aspirated uterine fluid were successfully determined. What is known already: Embryo culture conditions in the laboratory impact key outcomes of IVF/ICSI treatments, such as the quality of the embryos and […]
- Abnormal lipid metabolism in Alzheimer's disease (AD) was first documented by Alois Alzheimer in his early observations of patients with a then unrecognized brain disease, which he noted was characterized by a significant presence of "adipose inclusions" or "lipoid granules". Despite this early recognition, until recently the significance of abnormal lipid metabolism in AD has been largely overlooked by the scientific community for decades, highlighting a critical gap in our understanding of this complex disease. In the past decade, numerous […]
- Cells are subjected to dynamic mechanical environments which impart forces and induce cellular responses. In age-related conditions like pulmonary fibrosis, there is both an increase in tissue stiffness and an accumulation of senescent cells. While senescent cells produce a senescence-associated secretory phenotype (SASP), the impact of physical stimuli on both cellular senescence and the SASP is not well understood. Here, we show that mechanical tension, modeled using cell culture substrate rigidity, influences senescent cell markers like SA-{beta}-gal and secretory phenotypes. […]
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00350
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00403
- ABSTRACT To characterize pharmacokinetic profile of sennoside A in rats after intravenous and oral administration, a simple and sensitive liquid chromatography tandem mass spectrometry method was established and validated for quantitative determination of sennoside A in rat plasma. After prepared by protein precipitation with acetonitrile, sennoside A and internal standard were separated on a Waters ACQUITY HSS T3 (2.1 × 100 mm, 1.8 μm) column using acetonitrile and 5-mM ammonium acetate in water as mobile phase by gradient elution. The method showed excellent linearity […]
- ABSTRACT Mass spectrometry imaging (MSI) technologies are widely used today to study the in situ spatial distributions for a variety of analytes. As these technologies advance, the pursuit of higher resolution in MSI has intensified. The limitation of direct desorption/ionization is its insufficient ionization, posing a constraint on the advancement of high-resolution MSI technologies. The introduction of postionization process compensates the low ionization efficiency caused by sacrificing the desorption area while pursuing high spatial resolution, resolving the conflict between high […]
- ABSTRACT One of the great triumphs of mass spectrometry-based peptide and protein characterization is the characterization of their modifications as most modifications have a characteristic mass shift. What happens when the modification does not change the mass of the peptide? Here, the characterization of several peptide and proteins modifications that do not involve a mass shift are highlighted. Protein and peptide synthesis on ribosomes involves L-amino acids; however, posttranslational modifications (PTMs) can convert these L-amino acids into their D-isomers. As […]
- Analyst, 2024, Accepted ManuscriptDOI: 10.1039/D4AN01309C, Paper Open Access   This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.Akhila Ajith, Emrys Jones, Emily Prince, Drupad Trivedi, Giles N Johnson, Phillip J Milnes, Nicholas LockyerNewer and safer agrochemicals are always in demand to meet the increasing needs of a growing population for affordable food. Spatial chemical monitoring of the active mobility of an agrochemical…The content of this RSS Feed (c) The Royal Society of Chemistry