Analytical Chemistry (subscription required) has a unique article on the proteomic analysis of Renaissance paintings. The authors worked with microgram quantities of paint from Benedetto Bonfigli’s triptych, The Virgin and Child, St. John the Baptist, St. Sebastian (XVth century), and from Niccolo di Pietro Gerini’s, The Virgin and Child (XIVth century) and found proteins from the egg yolk and white that were used as binders in the paint. The presence of egg white is interesting in that the traditional recipe for tempera paint called for only the yolk, while the more radiant “tempera grassa” called for both the yolk and white.
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