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- Protein biomarkers in biofluids are highly sensitive indicators of prodromal cognitive impairment yet remain limited for primary prevention. Lipids, essential to brain structure and function, offer untapped prognostic value. Here, we identify a lipidomic signature in serum from asymptomatic PSEN1-E280A mutation carriers aged 6-40 years, that differentiate carriers from non-carriers with an AUC 80-90%. Similarly, to symptomatic carriers (older 41 years; 93%) and sporadic AD cases (85%), using high-resolution mass spectrometry. Latent profile analysis revealed lipid-based signatures of dementia risk […]
- ABSTRACT A simple, sensitive and efficient ultra performance liquid chromatographyâmass spectrometry (UPLCâMS/MS) method for simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma has been developed and validated. Using olmesartan-d4 and hydrochlorothiazide-13C-d2 as stable isotope-labeled internal standard (SIL-IS). Plasma samples were processed by protein precipitation. Separated on a ZORBAX Eclipse XDB-Phenyl column (150âmmâĂâ4.6âmm, 5âÎŒm) using a gradient elution with mobile phases methanol and 5âmM ammonium acetate. Detected by electrospray ionization (ESI) in negative ion mode of multiple reaction monitoring […]
- ABSTRACT Yi-Mai compound (YMHJ), a traditional Chinese medicine formulation, has demonstrated therapeutic efficacy against atherosclerosis (AS) in clinical observational studies. To elucidate its underlying pharmacological mechanisms, this investigation employed an integrated approach combining ultra-performance liquid chromatographyâelectrospray ionizationâtandem mass spectrometry (UPLC-ESI-MS/MS), network pharmacology analysis, and molecular docking validation. Through systematic chemical profiling, 2136 constituents were identified in YMHJ, with 33 prioritized as potential bioactive components using network pharmacology-based target prediction. Subsequent multiomics analysis revealed 67 disease-related targets and six hub genes […]
- In-situ cross-linking mass spectrometry reveals compartment-specific proteasomal interactions and structural heterogeneity
- Structural mechanisms of allosteric regulation in the human cis-prenyltransferase complex
- Symbiotic associations enable species to integrate complementary traits and adapt to novel environments. However, how the host and endosymbiont exchange nutrients remains poorly understood in most cases. Previously Paramecium bursaria cells were shown to reorganize lipid droplets to accommodate endosymbiotic Chlorella cells and interfering with lipid metabolism reduced the endosymbiont number. Here, we combined transcriptomics, lipidomics, imaging mass spectrometry, and stable-isotope tracing to investigate organic nutrient exchange in this symbiotic system. Our results reveal that endosymbiotic algae undergo extensive reprogramming […]
- Non-tailed phages remain an underexplored group in marine environments, as tailed phages have dominated sequence and culture collections. However, recent surveys indicate that non-tailed phages might be more abundant than tailed phages, and with different effects on microbial mortality and gene transfer. Here, we report the structural characterization of bacteriophage NO16, a non-tailed vibriophage, one of the simplest members of the Double Jelly Roll (DJR) lineage. Mass spectrometry analyses indicate that the virion is composed of at least nine different […]
- Publication date: Available online 28 November 2025Source: Analytica Chimica ActaAuthor(s): Ana Jano, AdriĂĄn Fuente-Ballesteros, M.Teresa MartĂn, Ana M. Ares, JosĂ© Bernal
- Publication date: Available online 28 November 2025Source: Analytica Chimica ActaAuthor(s): Alleigh N. Couch, Christopher M. Zall, J.Tyler Davidson
- We present the first application of a deuterated water metabolic labeling workflow coupled with data-independent acquisition (DIA) tandem mass spectrometry (MS/MS) for quantifying label enrichment in MS/MS to study protein turnover. The approach automates the turnover rate determination from combined precursor and fragment ions. The truncation of the observed isotope distributions of fragments is overcome by implementing an approach to determining the label enrichment from two mass isotopomers. The high redundancy of fragment ions provides a confident assessment for deuterium […]
- Publication date: Available online 27 November 2025Source: Journal of ProteomicsAuthor(s): Michele Martins, FĂĄbio CĂ©sar Sousa Nogueira, Magno Junqueira, Guillaume Nugue
- [ASAP] SpecQuality: A Tool for Reliable Spectral Quality Assessment in Proteomics and ProteogenomicsJournal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00168
- ABSTRACT Tannins are widespread specialized plant metabolites that contribute significantly to the polyphenol content of plant-based diets. Their effects on human and animal health vary depending on their structure, with potential benefits including antioxidative, antimicrobial, anthelmintic, and anticarcinogenic properties. Understanding tannin composition and quantity in plant products is essential, as their bioactivities are influenced by their functional groups. Mass spectrometry-based techniques excel in tannin analysis, offering both qualitative and quantitative insights. Combining ultrahigh-performance liquid chromatography with electrospray ionization and high-resolution […]
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c05933
- [ASAP] Phenotyping and Selection of Cells Using Mass Spectrometry and a Microfluidic Droplet PrinterAnalytical ChemistryDOI: 10.1021/acs.analchem.5c05032
- Publication date: Available online 26 November 2025Source: Journal of Chromatography AAuthor(s): Long Yuan
- Intracellular bacterial pathogens deploy effector proteins to hijack host membranes by targeting specific lipids. Phosphoinositides are key eukaryotic signaling molecules that govern membrane identity and dynamics, making them powerful levers for microbial interference. However, the bacterial repertoire of phosphoinositide-binding effectors remains largely undefined. In this study, we systematically identified such proteins using an expression library of 241 Legionella pneumophila effectors. We applied a combination of lipid bead pulldowns, mass spectrometry, colocalization with phosphoinositide biosensors, and protein-lipid overlay assays, leading to […]
- Publication date: Available online 27 November 2025Source: Analytica Chimica ActaAuthor(s): Yaping Li, Emir NazdrajiÄ, Wei Zhou, Janusz Pawliszyn
- Publication date: Available online 26 November 2025Source: Journal of Mass Spectrometry and Advances in the Clinical LabAuthor(s): Per Bengtson, Magnus Förnvik Jonsson
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00167
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00155
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00260
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c04847
- Correction to: Speciation analysis of fungi by liquid atmospheric pressure MALDI mass spectrometry
- Mascot Newsletter, November 2025:Top-3 quant with DDA-PASEF.ZooMS peptide markers for South American Xenarthrans.Migrating from Windows 10 to Linux could extend the lifetime of your hardware.https://www.matrixscience.com/nl/202511/newsletter.html#proteomics #massspec