Combined RSS Items
- Annexins are Ca2+ and membrane-binding proteins conserved across eukaryotes and involved in diverse membrane-associated processes, including unconventional protein secretion (UPS). In the intestinal parasite Giardia lamblia, annexins are represented by a family of 21 proteins termed alpha-giardins, all of which lack classical signal peptides yet are detected at the parasite surface and in the secretome. Here, we present a comprehensive functional analysis of alphaGs, with a particular focus on alpha 1-giardin, a leading vaccine candidate against giardiasis and a putative […]
- The regulation of gene expression requires precise control of chromatin-associated complexes that respond to diverse structural and epigenetic cues. The Rpd3 Large (Rpd3L) complex is a Sin3 histone deacetylase (HDAC) complex that dynamically adapt to chromatin states to reinforce transcriptional silencing, yet the mechanisms governing the catalytic activation in chromatin context-dependent manner remain unclear. Here we present the cryo-EM structure of Rpd3L bound to both mono- and di-nucleosome substrate at near-atomic resolution, uncovering a substrate-guided allosteric activation mechanism. Rpd3L adopts […]
- Spectral library search (SLS) is a major approach for peptide identification from tandem mass spectrometry data, with performance depending substantially on the accuracy of the underlying Spectrum-Spectrum Matching (SSM) scoring functions. However, detailed comparative studies remain limited by the absence of comprehensive benchmark datasets. We propose new methods to build SSM scoring functions benchmarks and construct a benchmark dataset with (i) eight query spectrum sets with varying noise level for 476,063 precursors, and (ii) three spectral libraries with experimental, de-noised […]
- Publication date: Available online 9 January 2026Source: International Journal of Mass SpectrometryAuthor(s): Luciana Rivera Molina, Robert Brand, Calum Bochenek, Chrys Wesdemiotis
- Publication date: Available online 8 January 2026Source: International Journal of Mass SpectrometryAuthor(s): Zhe Fang, Lei Wang, Kai Li, Chaogang Wang, Lizhi Ren, Yaqiang Li, Yihui Wang, Ming Chen, Haizhou Wang
- Publication date: Available online 8 January 2026Source: International Journal of Mass SpectrometryAuthor(s): Lasini Amunugama, Jared B. Shaw
- We investigated how seed proteolysis was enhanced by germination, by subsequent homogenisation (disrupting sprout compartments), and by co-incubation of homogenates from different species. Mass spectrometry of released peptides tracked proteolytic signatures from chickpea, lentil, mung and broccoli proteins, in soaked seeds, in sprouted seeds, and after sprout homogenisation followed by incubation alone or in mixture with other sprouts. The proteolytic signatures differed markedly among the four species, and in the different treatment conditions. After homogenisation, legumain-like cleavage (after asparagine) increased […]
- J. Anal. At. Spectrom., 2026, Accepted ManuscriptDOI: 10.1039/D5JA00337G, Technical NoteLouise Darricau, Jérôme Aléon, Maximilien Verdier, Virginie Sellier, Josselin Gorny, Smail Mostefaoui, Cyrielle Jardin, Arnaud Mangeret, Arnaud Duverger, Nicolas Ait-Ouabbas, David Suhard, Gilles Montavon, Alkiviadis GourgiotisThis study presents an analytical protocol for the quantitative imaging of 226Ra by Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS), based on the synthesis and characterization of barite standards enriched in…The content of this RSS Feed (c) The Royal Society of Chemistry
- Publication date: 15 May 2026Source: Talanta, Volume 302Author(s): Annika Haase, Gertrud Morlock
- Publication date: Available online 8 January 2026Source: TalantaAuthor(s): Xiaoyu Wei, Xudong Wang, Ming Xu
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00340
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00276
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c06533
- ABSTRACT The objective was to achieve accurate origins discriminations of flos of Lonicerae japonicae, especially those from the genuine and non-genuine producing areas. An integration strategy was proposed based on machine learning, combining ultra-high performance liquid chromatography-time-of-flight mass spectrometry and gas chromatography-ion mobility chromatography data fusion for geographical origins discrimination of flos of Lonicerae japonicae. Sixty-one batches of flos of Lonicerae japonicae samples from different origins were determined by ultra-high performance liquid chromatography-time-of-flight mass spectrometry and gas chromatography-ion mobility chromatography. […]
- Human myoglobin (hMYO) is a sensitive early biomarker for acute myocardial infarction, rising within 1~3 h after myocardial injury and offering high predictive value for excluding infarction. To standardize its measurement, a precise quantitative method combining magnetic bead extraction with liquid chromatography–isotope dilution tandem mass spectrometry (LC‑IDMS/MS) was established. 15NLabeled myoglobin was spiked into serum as an internal standard, and magnetic beads coated with monoclonal antibodies (mAbs) against hMYO were used for the extraction of hMYO from serum. The mAb employed […]
- Proteomics software is prone to filling up the disk with data and temp files, which can cause weird issues. This blog article lists the most common errors in Mascot products and how to free up disk space.https://www.matrixscience.com/blog/strange-software-failures-check-your-disk-space.html#proteomics #massspec
- To survive stressful conditions, bacteria enter the stringent response mediated by the magic spot nucleotides (MSN) guanosine 3', 5' bispyrophosphate (ppGpp) and guanosine 3'-diphosphate 5' triphosphate (pppGpp). Another group of so-called alarmones elevated under stress are dinucleoside polyphosphates (NpnNs), such as diadenosine triphosphate (Ap3A). How these two groups of alarmones intersect with one another is poorly understood. Here, we present a sensitive method using capillary electrophoresis coupled to mass spectrometry (CE-MS) with heavy isotope labeled internal references to separate and […]
- As part of the US HUPO sponsored "Pony Express" series highlighting speakers at the upcoming 2026 US HUPO meeting in St. Louis, Ben and Ben sit down to talk with Dr. Jeffrey Gordon, Washington University, who will be giving the opening plenary.keywords: microbiome; human gut; #24; philosophy of science
- The photoprotective efficacy of natural skin-active complexes is well recognized, yet their application is often hindered by the challenge of deciphering their complex, multi-component, and multi-target mechanisms. To bridge the gap between established phenotypes and molecular mechanisms, we developed an AI-driven integrated platform that combines phytochemical profiling, network pharmacology, and deep learning-based target prediction with rigorous biophysical validation. We applied this platform to investigate RRBE, a bioactive complex refined from red rice bran extract. In vivo clinical studies confirmed that […]
- Immunoglobulin light chains, a component of antibodies, can misfold and aggregate to cause systemic AL amyloidosis. Aggregation, including amyloid fibril formation, requires unfolding of the full-length light chain from its native state, and in most cases aberrant proteolysis. Small molecules that bind to the native state of light chains to stabilize them against conformational excursions and proteolysis are under development as drug candidates for AL amyloidosis. Since each patient has a unique light chain sequence, a challenge for candidate stabilizer […]
- Publication date: 1 May 2026Source: Talanta, Volume 301Author(s): Lucas Abruzzi, Jack Gillies, Erik T. Krogh, Daniel G. Beach, Chris G. Gill
- Anal. Methods, 2026, Accepted ManuscriptDOI: 10.1039/D5AY01566A, Paper Open Access   This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.Cécile Dionne Edoa, Ken Goeury, Justine Fontaine, Sébastien SauvéA sensitive method has been developed for the analysis of the three subclasses of dithiocarbamates (DTCs), (dimethyl dithiocarbamates (DMDs), ethylenebisdithiocarbamates (EBDs), propylenebisdithiocarbamates (PBD)) in berries and leafy vegetables using UHPLC/MS-MS….The content of this RSS Feed (c) The Royal Society of Chemistry
- Chemical proteomics is a powerful method to track proteins labelled by reactive small molecules in living cells on proteome-wide scale. The strategy relies on reactivity and specificity of bioorthogonal click reactions. Although a variety of bioorthogonal reactions have been developed to facilitate chemical proteomics, their reactivity and specificity might not be comparable with enzymatic reactions. Here we describe an iodoacetamide chloroalkane cysteine reactive probe that is, upon the reaction with nucleophilic cysteine of thioredoxin (TrxA), efficiently and specifically conjugated with […]
- Protein secretion is a fundamental mechanism for cellular coordination and signalling, with its dysregulation leading to widespread physiological dysfunction and disease. Immunoassay formats that utilise secondary antibody readouts are the current gold standard for measuring secreted proteins, offering high specificity and sensitivity, but relying on predefined protein panels that constrain the discovery of novel biology. We present a scalable mass spectrometry-based workflow that combines data-independent acquisition with ion mobility and parallel fragmentation to deliver rapid, global profiling of the secretome. […]
- Happy 2026 US Researchers! Here is your friendly reminder that the NIH did have time to invent not one – but 2 (two!) new required Biosketch formats. Ignore the one from the summer. Starting January 2026 your BioSketch MUST be made through the ScienV portal at the National Library of Medicine. (Yes that still exists, it's just the Goddard Library that is being shut down and the books thrown out).I assume the NIH came back from the longest shutdown in US […]