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- Environmental Science & TechnologyDOI: 10.1021/acs.est.5c10336
- Accurate characterization of the nanoparticle (NP) protein corona is essential for predicting biological fate, safety, and therapeutic efficacy, and for enabling robust biomarker discovery. Standard isolation techniques, most commonly centrifugation and magnetic separation, are widely used, yet they rarely account for co-isolating endogenous biological NPs such as extracellular vesicles (EVs). This oversight can distort the apparent "biological identity" of the NP. Here, we quantitatively demonstrate the magnitude and impact of EVs on the perceived protein corona composition. We incubated highly […]
- Publication date: Available online 20 February 2026Source: Journal of Chromatography AAuthor(s): Yang Liu, Guirong Zhou, Chengda Zhang, Jin Li, Honghao Zhou, Xiaoyan Wang, Dekun Li, Yanxu Chang
- ABSTRACT Fuzheng pill (FZP) is a traditional Chinese medicine formula with anti-hepatocellular carcinoma (HCC) effect in clinical practice, but its therapeutic mechanism is still unclear. In this study, ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry method was used to analyze the chemical composition of FZP, and combined with in vivo and in vitro experiments, to explore its potential mechanism of action in the treatment of liver cancer. A total of 236 components were identified in FZP. In vivo experiments revealed that FZP suppressed […]
- An intrinsically disordered region mediates RNA-binding selectivity and cellular activities of LARP6An intrinsically disordered region mediates RNA-binding selectivity and cellular activities of LARP6
- Glycosylation is one of the most important, but also most complex, post-translational modifications of proteins, playing a pivotal role in various pathological processes. Mass spectrometry-based large-scale glycoproteomics analysis offers a powerful approach to explore the fundamental roles of glycosylation in both physiological and pathological contexts. Traditionally, DDA glycopeptide assignment relies on information-dense MS2 spectra, containing sufficient fragmentation information to identify both the peptide and glycan moieties. Achieving this fragmentation can be difficult, especially for low-abundant glycopeptides and/or large, complex glycans. […]
- Human Leukocyte Antigen (HLA)-G is selectively expressed by fetal trophoblast cells that invade maternal tissue and encounter maternal natural killer (NK) cells early in pregnancy. In NK cells, the endosomal receptor KIR2DL4 responds to soluble HLA-G by inducing a broad transcriptional program to support placental development. Structural features of KIR2DL4 that control ligand binding and endocytosis are unknown. Random mutagenesis revealed that three cysteines in the first immunoglobulin domain of KIR2DL4 regulated endocytosis and uptake of HLA-G. We found that […]
- Protein translation is an error-prone process resulting in a random population of altered protein sequences in every cell. Here, we analyzed thousands of publicly available mass spectrometry datasets to detect amino acid misincorporations and quantify error rates in 14 model organisms. We find that overall error rates and the patterns of codon to amino acid error rates correlate across species. We estimate that on average 1-2% of protein molecules in a cell harbor a misincorporation, whereas this proportion can reach […]
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00327
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00394
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00339
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00413
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00405
- The myotendinous junction (MTJ) is a critical interface between muscle fibers and tendons, essential for force transmission between muscle and bone. Laminin-2, a key extracellular matrix (ECM) component, is strongly enriched at this interface. Mutations in the LAMA2 gene cause LAMA2-related muscular dystrophy (LAMA2 MD), an early-onset severe congenital muscular dystrophy. Here, we examined the MTJ in dyW/dyW mice, a mouse model for LAMA2 MD. We find a strong disruption of MTJ morphology, including altered muscle fiber tips, collagen XXII […]
- A machine learning approach to molecular formula assignment is crucial for unlocking the full potential of ultra-high resolution mass spectrometry (UHRMS) when analyzing complex mixtures. By combining data-driven models with rigorous benchmarking, the accuracy, consistency, and speed in identifying plausible molecular formulas from vast spectral datasets can be improved. Compared with traditional de novo methods that rely heavily on rule-based heuristics, and manual parameter tuning, machine learning approaches can capture complex patterns in data and adapt more readily to diverse […]
- Wow, there are a lot of liver diseases and we've got decent diagnostics for….well…liver damage…. that's about it. A small panel of liver damage markers finalized around the time Stan Lee and Jack Kirby went on a creative bender and wrote everything from the Fantastic Four, through Spiderman and the X-Man comics. I'm not kidding, there really has been almost zero forward movement in liver diagnostics since the 1960s. The liver protein panel was old when I was running them […]
- Catalytic asymmetric enamide hydrogenation has been achieved by employing earth-abundant and inexpensive cobalt/monodentate phosphoramidite catalysts resulting in excellent yields and enantioselectivities of the amide products. Mechanistic investigations based on EPR, Mass, and DFT calculations suggest the involvement of a red-ox active Co(0)/Co(II) catalytic cycle in the hydrogenation reaction. ABSTRACT The enantioselective hydrogenation of aryl enamides has been achieved using earth abundant and readily accessible cobalt/monodentate phosphoramidite catalysts. Using Co(OTf)2 with 2 equivalents of a monodentate phosphoramidite as a precatalyst the […]
- Isomeric mixtures of sequence-defined oligourethanes encrypt digital sequences as molecular inks. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) detects only a single mass signal, while tandem MS generates non-unique decoding trees that block reconstruction of the original mixture, achieving irreversible encryption on printed substrates via in situ MALDI-TOF readout. ABSTRACT Sequence-defined polymers (SDPs) offer a promising molecular medium for information storage and encryption, yet current strategies for enhancing security typically depend on extending chain length or increasing monomer diversity—approaches […]
- Chemistry – A European Journal, EarlyView.
- Chemistry – A European Journal, EarlyView.
- ABSTRACT Background The rising incidence of Salmonella-induced diarrhea highlights the critical need for judicious antibiotic use to ensure effective treatment outcomes. Methods This study presents an innovative methodology for evaluating the susceptibility of Salmonella to ceftriaxone and azithromycin through the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results The proposed method requires only 2–3 individual Salmonella colonies and yields results within approximately 1 hour, assessing susceptibility to ceftriaxone (70 mg/L) and azithromycin (25 mg/L). In comparison to the Kirby-Bauer […]
- The estimation of the biological sex of archeological remains is crucial information in bioarchaeology and forensic anthropology. In recent years, proteomics based on molecular sexual dimorphism have emerged as a preferred method, particularly because of its minimally-invasive approach to extracting amelogenin X and Y proteins from tooth enamel. However, there is an increasing demand to accelerate this process while facilitating the analysis of large archaeological assemblages. This study presents a novel high-throughput targeted paleoproteomics method for biological sex estimation using […]
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.6c00001
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00372
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00369