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- PROTEOMICS, EarlyView.
- Abstract The spatial metabolic analysis of tumor tissues following neoadjuvant chemotherapy (NAC) is critical for understanding chemotherapy-induced metabolic changes. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) offers a powerful tool for revealing spatially resolved metabolic profiles within tissues. However, necrotic regions in post-NAC tissues are fragile, creating challenges for sample preparation and MALDI MSI analysis. In this study, we introduce an optimized workflow employing conductive tape to stabilize tissue samples during sectioning and MALDI MSI analysis, preserving necrotic areas […]
- Analytical ChemistryDOI: 10.1021/acs.analchem.4c05961
- Publication date: Available online 21 February 2025Source: Analytica Chimica ActaAuthor(s): Anpu Wang, Taghi Sahraeian, Abraham K. Badu-Tawiah, Susan V. Olesik
- Publication date: Available online 20 February 2025Source: Journal of Chromatography BAuthor(s): Haihong Jia, Ruihong Li, Yahui Li, Fen Lu, Lan Ma, Xiujuan Xu
- As part of the US HUPO sponsored "Philly Express" series highlighting speakers at the upcoming 2025 US HUPO meeting in Philadelphia, Ben and Ben sit down to talk with Dr. Jesse Meyer, Cedars Sinai Medical Center, winner of the 2025 Robert J. Cotter New Investigator Award.
- THIS is the study that I've been waiting for. I don't know how it wasn't blowing up the interwebs when it dropped, but here it is.
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00448
- Analytical ChemistryDOI: 10.1021/acs.analchem.4c04516
- Journal of Mass Spectrometry, Volume 60, Issue 3, March 2025.
- ABSTRACT Metabolomics, a powerful discipline within systems biology, aims at comprehensive profiling of small molecules in biological samples. The challenges of biological sample complexity are addressed through innovative sample preparation methods, including solid-phase extraction and microextraction techniques, enhancing the detection and quantification of low-abundance metabolites. Advances in chromatographic separation, particularly liquid chromatography (LC) and gas chromatography (GC), coupled with high-resolution (HR) mass spectrometry (MS), have significantly improved the sensitivity, selectivity, and throughput of metabolomic studies. Cutting-edge techniques, such as ion-mobility […]
- ABSTRACT The widespread use of organophosphorus pesticides (OPs) has raised significant environmental and health concerns due to their residues in soil and potential entry into the food chain. This study introduced chiral analysis methods for four OPs—methamidophos (METHP), dipterex (DIP), malathion (MALA), and isothiophos-methyl (ISOME)—using liquid chromatography–tandem mass spectrometry (LC–MS/MS) with cellulose-based chiral columns. Three distinct methods were established: one for METHP, another for DIP, and a third for MALA and ISOME. Key chromatographic variables, including organic mobile phases and […]
- Publication date: Available online 19 February 2025Source: Analytica Chimica ActaAuthor(s): Lorenzo Toma, Maurizio Piergiovanni, Silvia Gentili, Monica Mattarozzi, Maria Careri, Encarnación Moyano
- Publication date: Available online 19 February 2025Source: Analytica Chimica ActaAuthor(s): Ids B. Lemmink, Sipke Bosma, Toine F.H. Bovee, Han Zuilhof, Gert IJ. Salentijn
- Publication date: Available online 19 February 2025Source: Analytica Chimica ActaAuthor(s): Kai Hua, Jiahao He, Xingtong Li, Congyi Zheng, Lingrui Ji, Qingyan Wu, Xi Chen, Ke Gao, Wei Wei, Liping Lu
- Publication date: Available online 19 February 2025Source: Analytica Chimica ActaAuthor(s): Ana M. Pedraza-Soto, Rafael Lucena, Soledad Cárdenas
- Publication date: Available online 19 February 2025Source: Analytica Chimica ActaAuthor(s): Yi-Ying Wu, Chin-Pao Chiu, Yu-Chie Chen
- Publication date: Available online 18 February 2025Source: TalantaAuthor(s): Sifang Wu, Feng Xu, Yong Wu, Chenlu Wang, Wenqing Gao, Renlu Han, Jiancheng Yu, Keqi Tang
- Publication date: Available online 8 February 2025Source: TalantaAuthor(s): Sandra M. Camunas-Alberca, Ameer Y. Taha, Ana Gradillas, Coral Barbas
- Publication date: Available online 19 February 2025Source: Journal of Chromatography BAuthor(s): Huixiao Duo, Liyun Wang, Pei Lu, Xiaodan Zhang, Mingcong Zheng, Hanying Song, Juan Zhou, Wenzhuo Lei, Shushu Ding, Jie Jia Li, Junxu Li, Qing Zhu
- Publication date: 15 April 2025Source: Journal of Proteomics, Volume 314Author(s):
- Publication date: Available online 19 February 2025Source: Journal of ProteomicsAuthor(s): Sara V. Villa-Arias, José Alberto Mendivil-de la Ossa, Frank W. Avila, Steve Dorus, Catalina Alfonso-Parra
- ABSTRACT Monosaccharides play a central role in metabolic networks and in the biosynthesis of glycomolecules, which perform essential functions across all domains of life. Thus, identifying and quantifying these building blocks is crucial in both research and industry. Routine methods have been established to facilitate the analysis of common monosaccharides. However, despite the presence of common metabolites, most organisms utilize distinct sets of monosaccharides and derivatives. These molecules therefore display a large diversity, potentially numbering in the hundreds or thousands, […]
- ABSTRACT Pectolinarin is a flavonoid compound known for its wound-healing properties, including anti-inflammatory and antibacterial effects. In this study, we employed UPLC-MS/MS to quantify pectolinarin in rat plasma and investigate its pharmacokinetics. Plasma samples were processed using an acetonitrile precipitation method. Chromatographic separation was performed on a UPLC BEH column with a gradient mobile phase of acetonitrile-water (containing 0.1% formic acid). Detection was carried out using electrospray ionization (ESI) tandem mass spectrometry in multiple reaction monitoring (MRM) mode with positive […]
- ABSTRACT Filgotinib (FLG) is a Janus kinase 1 inhibitor and is metabolized to an active metabolite, GS-829845. There is no report on the method for simultaneous quantification of FLG and GS-829845 in clinical samples. We developed a liquid chromatography–tandem mass spectrometry method for simultaneous determination of FLG and GS-829845 in patient plasma. FLG and GS-829845 were extracted from an aliquot of 50 μL of human plasma by simple deproteinization using methanol. Chromatographic separation was performed using a Shim-pack Scepter C18-120 column […]