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- Â This isn't super new, but a colleague sent it to me and it's really really cool.The liver is really weird and even though from a microscopic level it looks like a bag of square (they like "cuboidal") cells all stacked like bricks row after row forever, these cells are very different depending on where they are. These big bricks of cells are also packed full of mitochondria and may have hundreds of them per cell. This group used spatial sorting […]
- The 26S proteasome is the endpoint of the ubiquitin-proteasome system, an essential pathway for maintaining cellular homeostasis through targeted degradation of misfolded, damaged, and obsolete proteins. Substrates labeled with ubiquitin are directed to the 26S proteasome by binding to one or more ubiquitin receptors. However, ubiquitin-dependent degradation occurs even when the canonical receptor sites are mutated, suggesting the presence of additional, unidentified binding sites. Here we created photo-crosslinkable probes for ubiquitin interactions by incorporating the unnatural amino acid p-benzoyl-L-phenylalanine into […]
- Pumilio (PUM) proteins are conserved RNA-binding proteins that control mRNAs involved in development, proliferation, and stem cell differentiation. Human PUM1 and PUM2 repress targets by recruiting the CCR4-NOT deadenylase complex through a metazoan-specific N-terminal repression domain (RD3), which is predicted to be intrinsically disordered. Here we dissect RD3 using cell-based reporter assays, protein interaction assays with recombinant proteins, and crosslinking mass spectrometry. We identify multiple short RD3 peptides that are sufficient for repression and bind directly to the C-terminal NOT […]
- Publication date: Available online 20 December 2025Source: Journal of Chromatography AAuthor(s): Anna Tekieli, Mariusz Kowalczyk, SĹawomir Wybraniec, Aneta SpĂłrna-Kucab
- Publication date: Available online 19 December 2025Source: Journal of Chromatography AAuthor(s): Ling Liu, Yizhe Zhu, Zitao Chai, Ruifen Zheng, Zhiqiang Zeng, Caiming Tang
- Isotope dilution mass spectrometry (IDMS) is a critical technique for the quantification of protein biomarkers in complex biological samples, enabling sensitive measurement through integration with sample preparation to eliminate matrix interference. Nevertheless, the interchangeability between distinct pretreatment strategies is poorly defined. Herein, two traceable IDMS methods, immune mass spectrometry (IMS) and direct mass spectrometry (DMS), that combine immunoaffinity or antibody-free pretreatment were established for the quantification of plasma ProGRP, which is a recognized biomarker for small cell lung cancer. The […]
- Mass spectrometry imaging (MSI) is a powerful analytical technique suited for simultaneously measuring and assigning functional roles of multiple analytes directly from intact tissue sections. MSI acquisitions often lead to highly complex datasets with numerous isobaric species. Ion mobility (IM) spectrometry crucially helps to unravel these datasets by providing an orthogonal separation thus supplementing the lack of chromatographic separation in MSI. However, the rich, multidimensional data produced by IM-MSI investigations, combined with the lack of comprehensive software solutions that support […]
- Due to the demand in alternative medicine to treat various diseases, medicinal plants as ancient remedies gained more recent interest for the discovery and isolation of active compounds (Int J Environ Res Public Health. 2020;17(10):3376, J Ethnopharmacol. 2017;199:161-167). As these plant matrices contain an enormous range of metabolites, the challenge lies in the selection and application of separation techniques capable of resolving such complexity. For this reason, comprehensive two-dimensional liquid chromatography (LC ĂÂ LC), due to the increased peak capacity compared […]
- Raman spectroscopy allows rapid, non-invasive characterization of molecular changes in biological tissues, making it a promising tool for ecotoxicology. This study evaluates the potential of Raman spectroscopy combined with chemometrics for detecting changes induced by cadmium in the tissues of Mytilus edulis, a mussel species widely used in environmental monitoring. Adult mussels were exposed to two cadmium concentrations (50Â Âľg/L and 500Â Âľg/L) for 7 days. The cadmium concentrations in water and tissues of mussels (gills and mantle) were quantified by inductively […]
- Publication date: Available online 20 December 2025Source: Analytica Chimica ActaAuthor(s): Tapeesh Bharti, Anuj Kumar Rathi, Vivek Mishra
- Rapid Communications in Mass Spectrometry, Volume 40, Issue 5, 15 March 2026.
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00289
- Publication date: Available online 18 December 2025Source: Journal of Chromatography AAuthor(s): Quang-Dong Bui, Willy Verluyten, Bart Noten, Tiny Deschrijver, Sebastiaan Eeltink
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c06913
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c05530
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c05115
- ABSTRACT ErdongâYangxin oral liquid (EYOL) is a traditional Chinese medicine (TCM) prescription that demonstrates clinical efficacy in treating depression, yet its comprehensive chemical profile and quality-control markers remain insufficiently studied. This study aimed to systematically characterize its chemical composition and establish a robust method for quality assessment. First, ultrahigh-performance liquid chromatography (UHPLC)âlinear ion trap quadrupoleâOrbitrap mass spectrometry enabled the tentative identification of 149 compounds, primarily iridoid glycosides, flavonoids, and saponins. Subsequently, network pharmacology analysis predicted several components, such as dioscin, […]
- ABSTRACT Tetrahydromethyltestosterone (THMT) and 20-hydroxymethyl-18-nortetrahydromethyltestosterone (20OHnorTHMT) are metabolites of the anabolic androgenic steroids methyltestosterone and metandienone. Both molecular structures are used as markers in anti-doping analysis. There are eight reasonable diastereomeric structures of each group relevant for metabolic purposes. Highly sophisticated mass spectrometers fail to confidently differentiate these closely related, yet non-isomeric and non-isobaric groups of molecules. Due to the low abundance of the molecular ion, high-resolution mass spectrometry provides shared fragment ions that challenge identification by extracted ion chromatograms […]
- Mesoporous high-entropy oxides enable metabolic fingerprinting for pancreatic cancer
- Mass spectrometry (MS) and topâdown proteomics (TDP) have emerged as powerful tools to decipher proteoform-level information, advancing biological insight and translational research. This study unites leaders in capillary electrophoresis (CE) and MS from multiple institutions across the globe to showcase the reproducibility, unique information, and predictability of CE-MS for TDP analysis for a diverse set of platforms. Abstract Mass spectrometry (MS)-based topâdown proteomics (TDP) has emerged as a powerful tool for characterizing proteoforms to advance both fundamental and translational research. […]
- Simultaneous determination of vitamin B12 and cobalt (II) in infant milk power using liquid chromatography-inductively coupled plasma mass spectrometry
- đ Seasonâs Greetings & GratitudeThanks for choosing us in 2025.Wishing you a peaceful holiday season and a successful new year â from our team to yours!#mswil #proteomics #LCMS #MassSpec #MassSpectrometry #Proteomics #Chemistry #BioMedical #Pharmancy #Metabolomics #Physics #MSWil #MakingScienceWork
- Glycoproteomics data are rapidly accumulating due to advances in mass spectrometry instrumentation and the development of specialized search engines (e.g., pGlyco3, Glyco-Decipher) that enable identification of N-linked glycopeptide spectral matches (GPSMs) together with glycan structures. These advances have greatly expanded the scale and depth of N-linked glycopeptides; however, the intrinsic structural heterogeneity of glycosylation remains challenging to interpret. No existing tool provides a unified trait-based framework for analyzing N-linked GPSM data at both the glycosylation-site and protein levels. We developed […]
- Effects of processing on the polyphenolic content of Armagh Bramley apple products and pomace
- The STRiatin Interacting Phosphatases and Kinases (STRIPAK) complex is a conserved multiprotein module with functions in cell proliferation, cell growth, and cancer. Primarily examined through proteomic and in vitro studies, there is a relative lack of insight into the in vivo function of the STRIPAK complex during development. Motivated by the prominent interaction of STRIPAK and the CCM3 adapter protein, which is associated with the vascular disease cerebral cavernous malformation (CCM), we sought to examine the role of the conserved […]