{"id":985,"date":"2015-11-06T13:12:06","date_gmt":"2015-11-06T19:12:06","guid":{"rendered":"https:\/\/kermitmurray.com\/research\/?p=985"},"modified":"2024-02-20T09:34:49","modified_gmt":"2024-02-20T15:34:49","slug":"laser-desorption-sample-transfer-for-gas-chromatographymass-spectrometry","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2015\/11\/laser-desorption-sample-transfer-for-gas-chromatographymass-spectrometry\/","title":{"rendered":"Laser desorption sample transfer for gas chromatography\/mass spectrometry"},"content":{"rendered":"<p>C. A. Seneviratne, S. Ghorai &#038; K. K. Murray, Rapid Commun. Mass Spectrom. in press<br \/>\nDOI: <a href=\"http:\/\/dx.doi.org\/10.1002\/rcm.7419\" target=\"_blank\" rel=\"noopener noreferrer\">10.1002\/rcm.7419<\/a><\/p>\n<figure id=\"attachment_995\" aria-describedby=\"caption-attachment-995\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2015\/11\/SPME_GCMS_TOC.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2015\/11\/SPME_GCMS_TOC-300x129.jpg\" alt=\"Schematic representation of the experimental configuration for laser desorption sample transfer to SPME fiber. The heated transfer line is held 1 mm above the sample surface and the SPME fiber is inserted into a tee in the tube and exposed to the flow.\" width=\"300\" height=\"129\" class=\"size-medium wp-image-995\" srcset=\"https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2015\/11\/SPME_GCMS_TOC-300x129.jpg 300w, https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2015\/11\/SPME_GCMS_TOC.jpg 600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><figcaption id=\"caption-attachment-995\" class=\"wp-caption-text\">Schematic representation of the experimental configuration for laser desorption sample transfer to SPME fiber. The heated transfer line is held 1 mm above the sample surface and the SPME fiber is inserted into a tee in the tube and exposed to the flow.<\/figcaption><\/figure>\n<p><strong>RATIONALE: <\/strong>Ambient mass spectrometry can detect small molecules directly, but complex mixtures can be a challenge. We have developed a method that incorporates small molecule separation based on laser desorption with 80 capture on a solid-phase microextraction (SPME) fiber for injection into a gas chromatography\/mass spectrometry 81 (GCMS) system.<\/p>\n<p><strong>METHODS:<\/strong> Samples on a metal target were desorbed by a 3 \u03bcm mid-infrared laser focused to a 250 \u03bcm spot and 1.2 mJ pulse energy. The desorbed material was aspirated into a metal tube suspended 1 mm above the laser spot and captured 84 on a SPME fiber. The collected material was injected into a GC\/MS instrument for analysis.<\/p>\n<p><strong>RESULTS:<\/strong> We have developed a versatile approach for ambient laser desorption sampling onto SPME for GC\/MS analysis. The performance of the laser desorption SPME capture GC\/MS system was demonstrated for small molecule 87 standards, a mixture of nitroaromatic explosives, and collected cigarette smoke.<\/p>\n<p><strong>CONCLUSIONS:<\/strong> The utility of ambient laser desorption sampling onto SPME for GC\/MS was demonstrated. The performance of the method was evaluated by preparing calibration standards of caffeine over a range from 200 to 90 1000 ng. Laser desorption ambient sampling of complex mixtures was accomplished using SPME GC\/MS. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>C. A. Seneviratne, S. Ghorai &#038; K. K. Murray, Rapid Commun. Mass Spectrom. in press DOI: 10.1002\/rcm.7419 RATIONALE: Ambient mass spectrometry can detect small molecules directly, but complex mixtures can be a challenge. We have developed a method that incorporates small molecule separation based on laser desorption with 80 capture on a solid-phase microextraction (SPME) &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/kermitmurray.com\/research\/2015\/11\/laser-desorption-sample-transfer-for-gas-chromatographymass-spectrometry\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Laser desorption sample transfer for gas chromatography\/mass spectrometry&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[79,134,5],"tags":[135,174,77],"class_list":["post-985","post","type-post","status-publish","format-standard","hentry","category-last","category-lsu","category-publication","tag-lsu","tag-paper","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/985","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=985"}],"version-history":[{"count":4,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/985\/revisions"}],"predecessor-version":[{"id":2062,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/985\/revisions\/2062"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=985"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=985"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=985"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}