{"id":2842,"date":"2021-08-06T14:15:53","date_gmt":"2021-08-06T19:15:53","guid":{"rendered":"https:\/\/kermitmurray.com\/research\/?p=2842"},"modified":"2024-02-20T09:36:49","modified_gmt":"2024-02-20T15:36:49","slug":"multimodal-label%e2%80%90free-monitoring","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2021\/08\/multimodal-label%e2%80%90free-monitoring\/","title":{"rendered":"Multimodal Label\u2010Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers"},"content":{"rendered":"\n

N. Mehta, S. Shaik, A. Prasad, A. Chaichi, S.P. Sahu, Q. Liu, S.M.A. Hasan, E. Sheikh, F. Donnarumma, K.K. Murray, X. Fu, R. Devireddy, M.R. Gartia, Multimodal Label\u2010Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers, Adv. Funct. Mater.<\/em>, (2021) 2103955; doi: 10.1002\/adfm.202103955<\/a><\/p>\n\n\n\n

Abstract<\/h4>\n\n\n\n
\"Schematic
Schematic overview of quantitative label-free imaging implemented at the single-cell level. a) Label-free monitoring of human adipose-derived stem cell (hASCs) differentiation to adipogenic stem cells using Raman spectroscopy; b) Metabolic imaging using second harmonic generation (SHG) of stem cells to detect differentiated stem cells from control cells; c) MALDI-mass spectrometry imaging of lipid in differentiated stem cells; d) Schematic arrangement showing the hyperspectral imaging (HSI) system. The HSI system captures the spatial and spectral signature at each pixel of the image to form the data cube.<\/figcaption><\/figure><\/div>\n\n\n\n

Stem cell-based therapies carry significant promise for treating human diseases. However, clinical translation of stem cell transplants for effective treatment requires precise non-destructive evaluation of the purity of stem cells with high sensitivity (<0.001% of the number of cells). Here, a novel methodology using hyperspectral imaging (HSI) combined with spectral angle mapping-based machine learning analysis is reported to distinguish differentiating human adipose-derived stem cells (hASCs) from control stem cells. The spectral signature of adipogenesis generated by the HSI method enables identifying differentiated cells at single-cell resolution. The label-free HSI method is compared with the standard techniques such as Oil Red O staining, fluorescence microscopy, and qPCR that are routinely used to evaluate adipogenic differentiation of hASCs. HSI is successfully used to assess the abundance of adipocytes derived from transplanted cells in a transgenic mice model. Further, Raman microscopy and multiphoton-based metabolic imaging is performed to provide complementary information for the functional imaging of the hASCs. Finally, the HSI method is validated using matrix-assisted laser desorption\/ionization-mass spectrometry imaging of the stem cells. The study presented here demonstrates that multimodal imaging methods enable label-free identification of stem cell differentiation with high spatial and chemical resolution.<\/p>\n","protected":false},"excerpt":{"rendered":"

N. Mehta, S. Shaik, A. Prasad, A. Chaichi, S.P. Sahu, Q. Liu, S.M.A. Hasan, E. Sheikh, F. Donnarumma, K.K. Murray, X. Fu, R. Devireddy, M.R. Gartia, Multimodal Label\u2010Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers, Adv. Funct. Mater., (2021) 2103955; doi: 10.1002\/adfm.202103955 Abstract Stem cell-based therapies carry significant promise for treating human … <\/p>\n

Continue reading “Multimodal Label\u2010Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers”<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":2843,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,134,175,5],"tags":[174],"class_list":["post-2842","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-applications","category-lsu","category-paper","category-publication","tag-paper","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2842","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2842"}],"version-history":[{"count":2,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2842\/revisions"}],"predecessor-version":[{"id":2846,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2842\/revisions\/2846"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/2843"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2842"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2842"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2842"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}