{"id":2752,"date":"2021-07-22T10:11:35","date_gmt":"2021-07-22T15:11:35","guid":{"rendered":"https:\/\/kermitmurray.com\/research\/?p=2752"},"modified":"2024-02-20T09:36:49","modified_gmt":"2024-02-20T15:36:49","slug":"spatially-resolved-analysis-of-pa-biofilm-by-last","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2021\/07\/spatially-resolved-analysis-of-pa-biofilm-by-last\/","title":{"rendered":"Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer"},"content":{"rendered":"\n<p>A.C. Pulukkody, Y.P. Yung, F. Donnarumma, K.K. Murray, R.P. Carlson, L. Hanley, Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer, <em>PLoS One<\/em>, <strong>16<\/strong> (2021) e0250911, doi: <a href=\"https:\/\/doi.org\/10.1371\/journal.pone.0250911.g001\">10.1371\/journal.pone.0250911<\/a><\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Abstract<\/h4>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2021\/10\/journal.pone_.0250911.g001-1024x936.png\" alt=\"laser ablation sample transfer apparatus.\" class=\"wp-image-2753\" width=\"512\" height=\"468\" srcset=\"https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2021\/10\/journal.pone_.0250911.g001-1024x936.png 1024w, https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2021\/10\/journal.pone_.0250911.g001-300x274.png 300w, https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2021\/10\/journal.pone_.0250911.g001-768x702.png 768w, https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2021\/10\/journal.pone_.0250911.g001-1568x1433.png 1568w\" sizes=\"auto, (max-width: 512px) 100vw, 512px\" \/><figcaption>Schematic of laser ablation sample transfer (LAST) apparatus. A mid-IR laser beam is directed in transmission geometry through a glass slide to\nirradiate a biofilm. The spallated material is collected on a microcentrifuge tube for further processing.<\/figcaption><\/figure><\/div>\n\n\n\n<p>Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth<br>\ngives rise to changes in phenotype. There has been long term interest in identifying spatial<br>\ndifferences during biofilm development including clues that identify chemical heterogeneity.<br>\nLaser ablation sample transfer (LAST) allows site-specific sampling combined with label<br>\nfree proteomics to distinguish radially and axially resolved proteomes for Pseudomonas aeruginosa<br>\nbiofilms. Specifically, differential protein abundances on oxic vs. anoxic regions of a<br>\nbiofilm were observed by combining LAST with bottom up proteomics. This study reveals a<br>\nmore active metabolism in the anoxic region of the biofilm with respect to the oxic region for<br>\nthis clinical strain of P. aeruginosa, despite this organism being considered an aerobe by<br>\nnature. Protein abundance data related to cellular acclimations to chemical gradients<br>\ninclude identification of glucose catabolizing proteins, high abundance of proteins from arginine<br>\nand polyamine metabolism, and proteins that could also support virulence and environmental<br>\nstress mediation in the anoxic region. Finally, the LAST methodology requires only a<br>\nfew mm2 of biofilm area to identify hundreds of proteins.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A.C. Pulukkody, Y.P. Yung, F. Donnarumma, K.K. Murray, R.P. Carlson, L. Hanley, Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer, PLoS One, 16 (2021) e0250911, doi: 10.1371\/journal.pone.0250911 Abstract Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth gives rise to changes in phenotype. There has been &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/kermitmurray.com\/research\/2021\/07\/spatially-resolved-analysis-of-pa-biofilm-by-last\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":2753,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86,79,134,175,5],"tags":[174],"class_list":["post-2752","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-applications","category-last","category-lsu","category-paper","category-publication","tag-paper","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2752","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2752"}],"version-history":[{"count":2,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2752\/revisions"}],"predecessor-version":[{"id":3289,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2752\/revisions\/3289"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/2753"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2752"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2752"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2752"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}