{"id":2600,"date":"2003-07-19T22:00:09","date_gmt":"2003-07-20T03:00:09","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2600"},"modified":"2019-08-19T22:18:09","modified_gmt":"2019-08-20T03:18:09","slug":"two-laser-ir-and-uv-maldi","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2003\/07\/two-laser-ir-and-uv-maldi\/","title":{"rendered":"Two-laser infrared and ultraviolet matrix-assisted laser desorption\/ionization"},"content":{"rendered":"\n
<\/div>
<\/div><\/div>
\n
<\/div>
<\/div><\/div>
\n

M.W. Little, J.-K. Kim, K.K. Murray, “Two-laser infrared and ultraviolet matrix-assisted laser desorption\/ionization,” J. Mass Spectrom.<\/em>38<\/strong> (2003) 772\u2013777. doi:10.1002\/jms.494<\/a>. <\/p>\n\n\n\n

Abstract<\/h4>\n\n\n\n

Matrix\u2010assisted laser desorption\/ionization (MALDI) was performed using two pulsed lasers with wavelengths in the IR and UV regions. A 10.6 \u00b5m pulsed CO2<\/sub> laser was used to irradiate a MALDI target, followed after an adjustable delay by a 337 nm pulsed nitrogen laser. The sample consisted of a 2,5\u2010dihydroxybenzoic acid matrix and bovine insulin guest molecule. The pulse energy for both of the lasers was adjusted so that the ion of interest, either the matrix or guest ion, was not produced by either of the lasers alone. The delay time for maximum ion yield occurs at 1 \u00b5s for matrix and guest ions and the signal decayed to zero in \u223c400 \u00b5s. A mechanism is presented for enhanced UV MALDI ion yield following the IR laser pulse based on transient heating.<\/p>\n\n\n\n

\"Two-laser<\/a>
Schematic layout of the two-laser IR\/UV MALDI experiments using a linear time-of-flight mass spectrometer (TOF MS). The infrared (IR) and ultraviolet (UV) lasers are directed at the same target spot from opposite sides of the instrument. The computer (PC) controls the delay generator (DG) and digital oscilloscope (DO).<\/figcaption><\/figure><\/div>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n
<\/div>
<\/div><\/div>
\n
<\/div>
<\/div><\/div>
\n
\"Home-built<\/a>
Linear time-of-flight mass spectrometer<\/figcaption><\/figure>\n<\/div><\/div>\n\n\n\n
<\/div>
<\/div><\/div>
\n
\"Linear<\/a>
Linear time-of-flight mass spectrometer source chamber<\/figcaption><\/figure>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":2603,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[81,134,5],"tags":[8,9,149,75],"class_list":["post-2600","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-fundamentals","category-lsu","category-publication","tag-infrared","tag-maldi","tag-mechanism","tag-ultraviolet","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2600","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2600"}],"version-history":[{"count":6,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2600\/revisions"}],"predecessor-version":[{"id":2607,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2600\/revisions\/2607"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/2603"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2600"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2600"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2600"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}