{"id":2584,"date":"2004-10-19T21:21:02","date_gmt":"2004-10-20T02:21:02","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2584"},"modified":"2019-08-19T21:28:34","modified_gmt":"2019-08-20T02:28:34","slug":"matrix-free-ir-soft-ldi","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2004\/10\/matrix-free-ir-soft-ldi\/","title":{"rendered":"Matrix-free infrared soft laser desorption\/ionization"},"content":{"rendered":"\n
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D. Rousell, S.M. Dutta, M.W. Little, K.K. Murray, Matrix-free infrared soft laser desorption\/ionization, J. Mass Spectrom.<\/em>39<\/strong> (2004) 1182\u20131189. doi:10.1002\/jms.706<\/a>.<\/p>\n\n\n\n

Abstract<\/h4>\n\n\n\n
\"Omniflex<\/a>
Bruker Omniflex with 2.94 \u00b5m Er:YAG IR laser<\/figcaption><\/figure><\/div>\n\n\n\n

Infrared soft laser desorption\/ionization was performed using a 2.94 \u00b5m Er:YAG laser and a commercial reflectron time\u2010of\u2010flight mass spectrometer. The instrument was modified so that a 337 nm nitrogen laser could be used concurrently with the IR laser to interrogate samples. Matrix\u2010assisted laser desorption\/ionization (MALDI), laser desorption\/ionization and desorption\/ionization on silicon with UV and IR lasers were compared. Various target materials were tested for IR soft desorption ionization, including stainless steel, aluminum, copper, silicon, porous silicon and polyethylene. Silicon surfaces gave the best performance in terms of signal level and low\u2010mass interference. The internal energy resultant of the desorption\/ionization was assessed using the easily fragmented vitamin B12<\/sub>\u00a0molecule. IR ionization produced more analyte fragmentation than UV\u2010MALDI analysis. Fragmentation from matrix\u2010free IR desorption from silicon was comparable to that from IR\u2010MALDI. The results are interpreted as soft laser desorption and ionization resulting from the absorption of the IR laser energy by the analyte and associated solvent molecules.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

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\"IR<\/a>
IR Opolette OPO (front) and Er:YAG (back)<\/figcaption><\/figure>\n<\/div><\/div>\n\n\n\n
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\"IR<\/a>
IR Opolette OPO (left) and Er:YAG (right)<\/figcaption><\/figure>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":1839,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17,134,5],"tags":[146,8,145,125],"class_list":["post-2584","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-instrum","category-lsu","category-publication","tag-eryag","tag-infrared","tag-ldi","tag-time-of-flight","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2584","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2584"}],"version-history":[{"count":6,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2584\/revisions"}],"predecessor-version":[{"id":2590,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2584\/revisions\/2590"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/1839"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2584"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2584"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2584"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}