{"id":2556,"date":"2004-10-17T18:22:52","date_gmt":"2004-10-17T23:22:52","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2556"},"modified":"2024-02-20T09:39:00","modified_gmt":"2024-02-20T15:39:00","slug":"on-line-single-droplet-deposition-for-maldi-ms","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2004\/10\/on-line-single-droplet-deposition-for-maldi-ms\/","title":{"rendered":"On-line single droplet deposition for MALDI mass spectrometry"},"content":{"rendered":"\n<div class=\"wp-block-caxton-grid relative\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-columns caxton-grid-block\" style=\"padding-top:0;padding-left:0;padding-bottom:0;padding-right:0;grid-template-columns:repeat(12, 1fr)\" data-tablet-css=\"padding-left:em;padding-right:em;\" data-mobile-css=\"padding-left:em;padding-right:em;\">\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 12\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p>X. Zhang, D.A. Narcisse, K.K. Murray, On-line single droplet deposition for MALDI mass spectrometry,\u00a0<em>J. Am. Soc. Mass Spectrom.<\/em><strong>15<\/strong>\u00a0(2004) 1471\u20131477. doi:<a href=\"http:\/\/doi.org\/10.1016\/j.jasms.2004.06.016\">10.1016\/j.jasms.2004.06.016<\/a>.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Abstract<\/h4>\n\n\n\n<p>A single droplet generator was coupled with a rotating ball inlet matrix-assisted laser desorption\/ionization (MALDI) time of flight (TOF) mass spectrometer. Single droplets with 100 picoliter volume were ejected by a piezoelectric-actuated droplet generator and deposited onto a matrix-coated rotating stainless steel ball at atmospheric pressure. The single droplet deposit was transported to the vacuum side of the instrument where ionization was accomplished using a UV pulsed laser. Using this on-line interface, it was possible to obtain protonated molecule signal from as little as 10 fmol analyte.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"237\" height=\"300\" src=\"https:\/\/kermitmurray.com\/murray\/wp-content\/uploads\/2011\/02\/single-drop-inlet-237x300.jpg\" alt=\"\" class=\"wp-image-760\" srcset=\"https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2011\/02\/single-drop-inlet-237x300.jpg 237w, https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2011\/02\/single-drop-inlet.jpg 800w\" sizes=\"auto, (max-width: 237px) 100vw, 237px\" \/><figcaption>Diagram of the single droplet deposition device: A, 19 mm diameter stainless steel ball; B, drive shaft; C, gasket; D, ISO 100 flange; E, ground grid; F, droplet generation electronics; G, syringe pump for matrix solution; H, syringe pump for solvent; I, capillary; J, translation stage for capillary; K, droplet generator; L, translation stage for droplet generator; M, cleaning system (Teflon holder and felt); N, peek plastic tube for waste; O, syringe pump for analyte solution.<\/figcaption><\/figure><\/div>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":760,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17,134,85,5,78],"tags":[11],"class_list":["post-2556","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-instrum","category-lsu","category-microfluidic","category-publication","category-research-areas","tag-asms","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2556","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2556"}],"version-history":[{"count":5,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2556\/revisions"}],"predecessor-version":[{"id":2561,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2556\/revisions\/2561"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/760"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2556"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2556"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2556"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}