{"id":2551,"date":"2004-08-17T18:04:36","date_gmt":"2004-08-17T23:04:36","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2551"},"modified":"2019-08-17T18:10:01","modified_gmt":"2019-08-17T23:10:01","slug":"online-ce-maldi-tof-ms-using-a-rotating-ball-interface","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2004\/08\/online-ce-maldi-tof-ms-using-a-rotating-ball-interface\/","title":{"rendered":"Online CE-MALDI-TOF MS using a rotating ball interface"},"content":{"rendered":"\n
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H. Musyimi, D.A. Narcisse, X. Zhang, W. Stryjewski, S.A. Soper, K.K. Murray, Online CE-MALDI-TOF MS using a rotating ball interface, Anal. Chem.<\/em>76<\/strong> (2004) 5968\u20135973. doi:10.1021\/ac0489723<\/a>.<\/p>\n\n\n\n

Abstract<\/h4>\n\n\n\n
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A schematic representation of the rotating ball inlet interface for CE-MS indicating: ball A, driveshaft B, teflon gasket C, ISO-100 flange D, extraction lens E, matrix capillary F, buffer capillary G, anode and running buffer H, electrophoresis capillary I, cleaning solvent capillary J, felt pad and holder K and solvent drain L.<\/figcaption><\/figure><\/div>\n\n\n\n

We report on the construction and performance of a rotating ball interface for online coupling of capillary electrophoresis (CE) to matrix-assisted laser desorption ionization (MALDI) mass spectrometry with a time-of-flight (TOF) mass analyzer. The interface is based on a rotating stainless steel ball that transports samples from atmospheric pressure to the high vacuum of the mass spectrometer for desorption and ionization. The sample is deposited directly from a 50-\u03bcm-i.d. separation capillary onto the 19-mm ball that is rotating at 0.03 to 0.3 rpm. The sample is mixed online with matrix flowing from a separate 50-\u03bcm-i.d. capillary. The sample deposit dries before it is rotated past a polymer gasket and into the laser ionization region. Cleaning of the interface is accomplished using solvent-saturated felt, which cleans the ball surface after it rotates out of the ionization chamber. On-line CE\u2212MALDI is demonstrated, and the performance is evaluated with the analysis of a mixture of three peptides:\u2009 [Lsy8<\/sup>] vasopressin, substance P, and neurotensin. The rotating ball interface to MALDI-TOF MS demonstrated mass detection limit in the high femtomole range. The interface has negligible memory effect and shows no significant electrophoretic peak broadening when operated under optimized conditions.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

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A schematic diagram showing the top view of the chip to ball direct contact deposition: (A) sample reservoir, (B) waste reservoir, (C) buffer reservoir, (D) fluidic channel exit tip, (E) (E) ball. Panels on the right show two scanning electron micros- copy (SEM) images of (1) the brass mold insert and (2) an embossed microchip tip.<\/figcaption><\/figure>\n<\/div><\/div>\n\n\n\n
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Rotating ball inlet detail<\/figcaption><\/figure>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n
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Rotating ball inlet with capillary electrophoresis microfluidic chip.<\/figcaption><\/figure><\/div>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":758,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,85,5],"tags":[],"class_list":["post-2551","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lsu","category-microfluidic","category-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2551","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2551"}],"version-history":[{"count":4,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2551\/revisions"}],"predecessor-version":[{"id":2555,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2551\/revisions\/2555"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/758"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2551"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2551"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2551"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}