{"id":2524,"date":"2006-03-15T16:17:27","date_gmt":"2006-03-15T22:17:27","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2524"},"modified":"2024-02-20T09:39:00","modified_gmt":"2024-02-20T15:39:00","slug":"capillary-gel-chip-ir-ldi-ms","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2006\/03\/capillary-gel-chip-ir-ldi-ms\/","title":{"rendered":"Interfacing capillary gel microfluidic chips with infrared laser desorption mass spectrometry"},"content":{"rendered":"\n
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Y. Xu, M.W. Little, K.K. Murray, “Interfacing capillary gel microfluidic chips with infrared laser desorption mass spectrometry,” J. Am. Soc. Mass Spectrom.<\/em>17<\/strong> (2006) 469\u2013474. doi:10.1016\/j.jasms.2005.12.003<\/a>.<\/p>\n\n\n\n

Abstract<\/h4>\n\n\n\n
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Capillary gel microfluidic chip interfaced to laser desorption\/ionization (LDI) mass spectrometry with a time-of-flight mass analyzer.<\/figcaption><\/figure><\/div>\n\n\n\n

We report on the fabrication and performance of a gel microfluidic chip interfaced to laser desorption\/ionization (LDI) mass spectrometry with a time-of-flight mass analyzer. The chip was fabricated from poly(methylmethacrylate) with a poly(dimethyl siloxane) cover. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed in the channel of the microfluidic chip. After electrophoresis, the cover was removed and either the PDMS chip or the PMMA cover was mounted in a modified MALDI ion source for analysis. Ions were formed by irradiating the channel with 2.95 \u00b5m radiation from a pulsed optical parametric oscillator (OPO), which is coincident with IR absorption by N-H and O-H stretch of the gel components. No matrix was added. The microfluidic chip design allowed a decrease in the volume of material required for analysis over conventional gel slabs, thus enabling improvement in the detection limit to a pmol level, a three orders of magnitude improvement over previous studies in which desorption was achieved from an excised section of a conventional gel.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":762,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,85,5],"tags":[11,137,8],"class_list":["post-2524","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lsu","category-microfluidic","category-publication","tag-asms","tag-chip","tag-infrared","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2524","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2524"}],"version-history":[{"count":3,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2524\/revisions"}],"predecessor-version":[{"id":2527,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2524\/revisions\/2527"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/762"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2524"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2524"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2524"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}