{"id":2508,"date":"2004-01-14T21:50:35","date_gmt":"2004-01-15T03:50:35","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2508"},"modified":"2019-08-14T22:02:54","modified_gmt":"2019-08-15T03:02:54","slug":"direct-from-gel-ir-ldi","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2004\/01\/direct-from-gel-ir-ldi\/","title":{"rendered":"Direct from polyacrylamide gel infrared laser desorption\/ionization"},"content":{"rendered":"\n
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Y. Xu, M.W. Little, D.J. Rousell, J.L. Laboy, K.K. Murray, “Direct from polyacrylamide gel infrared laser desorption\/ionization,” Anal. Chem.<\/em> 76<\/strong> (2004) 1078\u20131082. doi:10.1021\/ac034879n<\/a>.<\/p>\n\n\n\n

Abstract<\/h4>\n\n\n\n
\"FT-IR-ATR<\/a>
FT-IR-ATR spectra of dry (black solid line) and wet\n(dashed line) polyacrylamide gel.<\/figcaption><\/figure><\/div>\n\n\n\n

The direct combination of gel electrophoresis and infrared laser desorption\/ionization time-of-flight mass spectrometry has been demonstrated. We present results for infrared laser desorption and ionization mass spectrometry of peptides and proteins directly from a polyacrylamide gel without the addition of a matrix. Analyte molecules up to 6 kDa were ionized directly from a vacuum-dried sodium dodecyl sulfate-polyacrylamide gel after electrophoretic separation. Mass spectra were obtained at the wavelength of 2.94 \u00b5m, which is consistent with IR absorption by N-H and O-H stretch vibrations of water and other constituents of the gel. A 5-nmol quantity of peptide or protein was loaded per gel slot, although it was possible to obtain mass spectra from a small fraction of the gel spot. This technique shows promise for the direct identification of both parent and fragment masses of proteins contained in polyacrylamide gels.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":2512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,85,5],"tags":[138,8],"class_list":["post-2508","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lsu","category-microfluidic","category-publication","tag-gel","tag-infrared","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2508","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2508"}],"version-history":[{"count":4,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2508\/revisions"}],"predecessor-version":[{"id":2516,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2508\/revisions\/2516"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/2512"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2508"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2508"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2508"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}