{"id":2467,"date":"2006-05-13T17:09:14","date_gmt":"2006-05-13T22:09:14","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2467"},"modified":"2019-08-13T17:10:50","modified_gmt":"2019-08-13T22:10:50","slug":"ir-maldi-ldi-im-otof-ms","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2006\/05\/ir-maldi-ldi-im-otof-ms\/","title":{"rendered":"IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry"},"content":{"rendered":"\n
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A.S. Woods, M.V. Ugarov, S.N. Jackson, T. Egan, H.-Y.J. Wang, K.K. Murray, J. A. Schultz, “IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry,” J. Proteome Res.<\/em> 5<\/strong> (2006) 1484\u20131487. doi:10.1021\/pr060055l<\/a>.<\/p>\n\n\n\n

Abstract<\/h4>\n\n\n\n
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Woods, Ugarov, Jackson, Egan, Wang, Murray, & Schultz, IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry, J. Proteome Res. 5 (2006) 1484.<\/figcaption><\/figure><\/div>\n\n\n\n

Most MALDI instrumentation uses UV lasers. We have designed a MALDI\u2212IM\u2212oTOF\u2212MS which employs both a Nd:YAG laser pumped optical parametric oscillator (OPOTEK, \u03bb = 2.8\u22123.2 \u03bcm at 20 Hz) to perform IR\u2212LDI or IR\u2212MALDI and a Nd:YLF laser (Crystalaser, \u03bb = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives a fast separation and analysis of biomolecules from complex mixtures in which ions of similar chemical type fall along well-defined \u201ctrend lines\u201d. Our data shows that ion mobility allows multiply charged monomers and multimers to be resolved; thus, yielding pure spectra of the singly charged protein ion which are virtually devoid of chemical noise. In addition, we have demonstrated that IR\u2212LDI produced similar results as IR\u2212MALDI for the direct tissue analysis of phospholipids from rat brain.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":2469,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,5],"tags":[8,107,9],"class_list":["post-2467","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lsu","category-publication","tag-infrared","tag-ion-mobility","tag-maldi","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2467","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2467"}],"version-history":[{"count":3,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2467\/revisions"}],"predecessor-version":[{"id":2471,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2467\/revisions\/2471"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/2469"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2467"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2467"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2467"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}