{"id":2316,"date":"2014-10-02T20:56:56","date_gmt":"2014-10-03T01:56:56","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2316"},"modified":"2019-08-19T21:42:30","modified_gmt":"2019-08-20T02:42:30","slug":"naldi-ms-for-bacteria-analysis","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2014\/10\/naldi-ms-for-bacteria-analysis\/","title":{"rendered":"Ambient laser ablation sample transfer with nanostructure-assisted laser desorption ionization mass spectrometry for bacteria analysis"},"content":{"rendered":"\n<div class=\"wp-block-caxton-grid relative\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-columns caxton-grid-block\" style=\"padding-top:0;padding-left:0;padding-bottom:0;padding-right:0;grid-template-columns:repeat(12, 1fr)\" data-tablet-css=\"padding-left:em;padding-right:em;\" data-mobile-css=\"padding-left:em;padding-right:em;\">\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 12\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p>J.M. Hayes, K.K. Murray, Ambient laser ablation sample transfer with nanostructure-assisted laser desorption ionization mass spectrometry for bacteria analysis, <em>Rapid Commun. Mass Spectrom.<\/em> <strong>28<\/strong> (2014) 2382\u20132384. doi:<a href=\"http:\/\/doi.org\/10.1002\/rcm.7023\">10.1002\/rcm.7023<\/a>.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Abstract<\/h4>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright\"><img loading=\"lazy\" decoding=\"async\" width=\"204\" height=\"300\" src=\"https:\/\/kermitmurray.com\/murray\/wp-content\/uploads\/2014\/10\/LAST-NALDI-Fig-1-204x300.jpg\" alt=\"LAST NALDI \" class=\"wp-image-2592\" srcset=\"https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2014\/10\/LAST-NALDI-Fig-1-204x300.jpg 204w, https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2014\/10\/LAST-NALDI-Fig-1-768x1130.jpg 768w, https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2014\/10\/LAST-NALDI-Fig-1-696x1024.jpg 696w, https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2014\/10\/LAST-NALDI-Fig-1.jpg 1553w\" sizes=\"auto, (max-width: 204px) 100vw, 204px\" \/><figcaption>MALDI mass spectrum of bacteria with CHCA matrix (a) E. coli and (b) B. cereus. The inset spectra show the low m\/z range.<\/figcaption><\/figure><\/div>\n\n\n\n<p>The direct analysis of bacteria phospholipids was accomplished by a combination of ambient sampling of bacterial colonies by laser ablation combined with a nanostructure-assisted laser desorption ionization (NALDI) MS. Bacteria from colonies of <em>E. coli <\/em>and <em>B. cereus<\/em> were irradiated with a pulsed infrared laser and the ablated material was collected in a solvent droplet. The droplet was deposited directly on a NALDI target and analyzed in a commercial time-of-flight (TOF) mass spectrometer, with no further treatment. The resulting mass spectra reveal distinctive peak patterns corresponding to the bacteria phospholipids.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":2592,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[84,79,134,5],"tags":[115,135,124,77,125],"class_list":["post-2316","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-forensics","category-last","category-lsu","category-publication","tag-bacteria","tag-lsu","tag-naldi","tag-publication","tag-time-of-flight","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2316","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2316"}],"version-history":[{"count":4,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2316\/revisions"}],"predecessor-version":[{"id":2595,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2316\/revisions\/2595"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/2592"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2316"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2316"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2316"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}