{"id":2297,"date":"2008-12-02T15:24:47","date_gmt":"2008-12-02T21:24:47","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2297"},"modified":"2024-02-20T09:39:00","modified_gmt":"2024-02-20T15:39:00","slug":"intact-and-top-down-characterization-of-biomolecules-and-direct-analysis-using-ir-maldi-ld-esi-coupled-to-ft-icr-ms","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2008\/12\/intact-and-top-down-characterization-of-biomolecules-and-direct-analysis-using-ir-maldi-ld-esi-coupled-to-ft-icr-ms\/","title":{"rendered":"Intact and Top-Down Characterization of Biomolecules and Direct Analysis Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Coupled to FT-ICR Mass Spectrometry"},"content":{"rendered":"\n<div class=\"wp-block-caxton-grid relative\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-columns caxton-grid-block\" style=\"padding-top:0;padding-left:0;padding-bottom:0;padding-right:0;grid-template-columns:repeat(12, 1fr)\" data-tablet-css=\"padding-left:em;padding-right:em;\" data-mobile-css=\"padding-left:em;padding-right:em;\">\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 12\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p>J.S. Sampson, K.K. Murray, D.C. Muddiman, Intact and Top-Down Characterization of Biomolecules and Direct Analysis Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Coupled to FT-ICR Mass Spectrometry, <em>J. Am. Soc. Mass Spectrom.<\/em> <strong>20<\/strong> (2009) 667\u2013673. doi:<a href=\"http:\/\/doi.org\/10.1016\/j.jasms.2008.12.003\">10.1016\/j.jasms.2008.12.003<\/a>.<\/p>\n\n\n\n<h5 class=\"wp-block-heading\">Abstract<\/h5>\n\n\n\n<p>We report the implementation of an infrared laser onto our previously reported matrix-assisted laser desorption electrospray ionization (MALDESI) source with ESI post-ionization yielding multiply charged peptides and proteins. Infrared (IR)-MALDESI is demonstrated for atmospheric pressure desorption and ionization of biological molecules ranging in molecular weight from 1.2 to 17 kDa. High resolving power, high mass accuracy single-acquisition Fourier transform ion cyclotron resonance (FT-ICR) mass spectra were generated from liquid- and solid-state peptide and protein samples by desorption with an infrared laser (2.94&nbsp;<em>\u00b5<\/em>m) followed by ESI post-ionization. Intact and top-down analysis of equine myoglobin (17 kDa) desorbed from the solid state with ESI post-ionization demonstrates the sequencing capabilities using IR-MALDESI coupled to FT-ICR mass spectrometry. Carbohydrates and lipids were detected through direct analysis of milk and egg yolk using both UV- and IR-MALDESI with minimal sample preparation. Three of the four classes of biological macromolecules (proteins, carbohydrates, and lipids) have been ionized and detected using MALDESI with minimal sample preparation. Sequencing of O-linked glycans, cleaved from mucin using reductive&nbsp;<em>\u03b2<\/em>-elimination chemistry, is also demonstrated.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,5],"tags":[11,135,77],"class_list":["post-2297","post","type-post","status-publish","format-standard","hentry","category-lsu","category-publication","tag-asms","tag-lsu","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2297","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2297"}],"version-history":[{"count":2,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2297\/revisions"}],"predecessor-version":[{"id":2299,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2297\/revisions\/2299"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2297"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2297"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2297"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}