{"id":2271,"date":"2011-07-29T20:40:05","date_gmt":"2011-07-30T01:40:05","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2271"},"modified":"2019-08-03T16:11:16","modified_gmt":"2019-08-03T21:11:16","slug":"maldi-im-tofms-of-bacteria","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2011\/07\/maldi-im-tofms-of-bacteria\/","title":{"rendered":"Matrix Assisted Laser Desorption Ionization Ion Mobility Time-of-Flight Mass Spectrometry of Bacteria"},"content":{"rendered":"\n<div class=\"wp-block-caxton-grid relative\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-columns caxton-grid-block\" style=\"padding-top:0;padding-left:0;padding-bottom:0;padding-right:0;grid-template-columns:repeat(12, 1fr)\" data-tablet-css=\"padding-left:em;padding-right:em;\" data-mobile-css=\"padding-left:em;padding-right:em;\">\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 12\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p>J.M. Hayes, L.C. Anderson, J.A. Schultz, M.V. Ugarov, T.F. Egan, E.K. Lewis, V. Womack, A. S. Woods, S. N. Jackson, R. H. Hauge, K. Kittrell, S. Ripley, K. K. Murray, &#8220;Matrix Assisted Laser Desorption Ionization Ion Mobility Time-of-Flight Mass Spectrometry of Bacteria,&#8221; in: C. Fenselau, P. Demirev (Eds.), <em>Rapid Characterization of Microorganisms by Mass Spectrometry<\/em>, American Chemical Society, Washington, DC, 2011: pp. 143\u2013160. doi:<a href=\"http:\/\/doi.org\/10.1021\/bk-2011-1065.ch009\">10.1021\/bk-2011-1065.ch009<\/a>.<\/p>\n\n\n\n<h5 class=\"wp-block-heading\">Abstract<\/h5>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright is-resized\"><a href=\"https:\/\/kermitmurray.com\/murray\/?attachment_id=2273\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/kermitmurray.com\/murray\/wp-content\/uploads\/2019\/07\/Bacteria-IMMS-300x233.gif\" alt=\"Bacteria IMMS\" class=\"wp-image-2273\" width=\"225\" height=\"175\"\/><\/a><figcaption>UV MALDI-IM-TOF MS 2-D contour plot of lipopeptide products with\nNa+ and K+ adducts from whole cell B. subtilis ATCC 6633<\/figcaption><\/figure><\/div>\n\n\n\n<p>Ion mobility mass spectrometry (IMMS) was combined with matrix assisted laser desorption ionization (MALDI) for the analysis of whole cell bacteria. Whole cell <em>Bacillus subtilis<\/em> ATCC 6633 and <em>Escherichia coli<\/em> strain W ATCC 9637 bacteria were prepared with a 1:2 analyte to matrix (CHCA) ratio and deposited using the dried-droplet method. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) studies and matrix assisted laser desorption ionization ion mobility time-of-flight mass spectrometry (MALDI-IM-TOF MS) were conducted in parallel to assess the effectiveness of MALDI-IM-TOF MS for microorganism identification. Ribosomal proteins from <em>Escherichia coli<\/em> strain W ATCC 9637 were observed and assigned using the Rapid Microorganism Identification Database. Isoforms of lipopeptide products and a lantibiotic from the <em>Bacillus subtilis<\/em> ATCC 6633 species were found in the range 1000\u20133500 <em>m\/z<\/em> and were identified using MALDI-IM-TOF MS and compared to MALDI-TOF MS data for confirmation. Vacuum ultraviolet (VUV) post-ionization MALDI-IM-TOF MS showed that additional information on lipopeptides could be obtained and used for identification.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":2273,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,5],"tags":[115,107,135,9,77],"class_list":["post-2271","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lsu","category-publication","tag-bacteria","tag-ion-mobility","tag-lsu","tag-maldi","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2271","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2271"}],"version-history":[{"count":3,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2271\/revisions"}],"predecessor-version":[{"id":2275,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2271\/revisions\/2275"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/2273"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2271"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2271"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2271"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}