{"id":2233,"date":"2018-11-27T21:05:09","date_gmt":"2018-11-28T03:05:09","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2233"},"modified":"2024-02-20T09:34:48","modified_gmt":"2024-02-20T15:34:48","slug":"ion-mobility-deconvolution","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2018\/11\/ion-mobility-deconvolution\/","title":{"rendered":"Broadband ion mobility deconvolution for rapid analysis of complex mixtures"},"content":{"rendered":"\n<p>M.E. Pettit, M.R. Brantley, F. Donnarumma, K.K. Murray, T. Solouki, Broadband ion mobility deconvolution for rapid analysis of complex mixtures, <em>Analyst<\/em>. <strong>143<\/strong> (2018) 2574\u20132586. doi:<a href=\"http:\/\/doi.org\/10.1039\/c8an00193f\">10.1039\/c8an00193f<\/a>.<\/p>\n\n\n\n<div class=\"wp-block-caxton-grid relative\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-columns caxton-grid-block\" style=\"padding-top:0;padding-left:0;padding-bottom:0;padding-right:0;grid-template-columns:repeat(12, 1fr)\" data-tablet-css=\"padding-left:em;padding-right:em;\" data-mobile-css=\"padding-left:em;padding-right:em;\">\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 12\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p>High resolving power ion mobility (IM) allows for accurate characterization of complex mixtures in high-throughput IM mass spectrometry (IM-MS) experiments. We previously demonstrated that pure component IM-MS data can be extracted from IM unresolved post-IM\/collision-induced dissociation (CID) MS data using automated ion mobility deconvolution (AIMD) software [Matthew Brantley, Behrooz Zekavat, Brett Harper, Rachel Mason, and Touradj Solouki, <em>J. Am. Soc. Mass Spectrom.<\/em>, 2014, <strong>25<\/strong>, 1810-1819]. In our previous reports, we utilized a quadrupole ion filter for m\/z-isolation of IM unresolved monoisotopic species prior to post-IM\/CID MS. Here, we utilize a broadband IM-MS deconvolution strategy to remove the m\/z-isolation requirement for successful deconvolution of IM unresolved peaks. Broadband data collection has throughput and multiplexing advantages; hence, elimination of the ion isolation step reduces experimental run times and thus expands the applicability of AIMD to high-throughput bottom-up proteomics. We demonstrate broadband IM-MS deconvolution of two separate and unrelated pairs of IM unresolved isomers (viz., a pair of isomeric hexapeptides and a pair of isomeric trisaccharides) in a simulated complex mixture. Moreover, we show that broadband IM-MS deconvolution improves high-throughput bottom-up characterization of a proteolytic digest of rat brain tissue. To our knowledge, this manuscript is the first to report successful deconvolution of pure component IM and MS data from an IM-assisted data-independent analysis (DIA) or HDMS<SUP>E<\/sup> dataset.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>M.E. Pettit, M.R. Brantley, F. Donnarumma, K.K. Murray, T. Solouki, Broadband ion mobility deconvolution for rapid analysis of complex mixtures, Analyst. 143 (2018) 2574\u20132586. doi:10.1039\/c8an00193f.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,5],"tags":[107,135,108,174,77],"class_list":["post-2233","post","type-post","status-publish","format-standard","hentry","category-lsu","category-publication","tag-ion-mobility","tag-lsu","tag-mass-spectrometry","tag-paper","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2233","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2233"}],"version-history":[{"count":1,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2233\/revisions"}],"predecessor-version":[{"id":2234,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2233\/revisions\/2234"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2233"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2233"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2233"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}