{"id":2128,"date":"2014-10-20T11:50:50","date_gmt":"2014-10-20T16:50:50","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2128"},"modified":"2024-02-20T09:39:00","modified_gmt":"2024-02-20T15:39:00","slug":"2128","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2014\/10\/2128\/","title":{"rendered":"Tip-enhanced laser ablation sample transfer for biomolecule mass spectrometry"},"content":{"rendered":"\n
<\/div>
<\/div><\/div>
\n
<\/div>
<\/div><\/div>
\n

S. Ghorai, C.A. Seneviratne, K.K. Murray, “Tip-enhanced laser ablation sample transfer for biomolecule mass spectrometry,” J. Am. Soc. Mass Spectrom.<\/em>26<\/strong> (2015) 63\u201370. doi:10.1007\/s13361-014-1005-x<\/a>.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

<\/div>
<\/div><\/div>
\n
<\/div>
<\/div><\/div>
\n

Abstract<\/em>: Atomic force microscope (AFM) tip-enhanced laser ablation was used to transfer molecules from thin films to a suspended silver wire for off-line mass spectrometry using laser desorption ionization (LDI) and matrix-assisted laser desorption ionization (MALDI). An AFM with a 30 nm radius gold-coated silicon tip was used to image the sample and to hold the tip 15 nm from the surface for material removal using a 355 nm Nd:YAG laser. The ablated material was captured on a silver wire that was held 300 \u03bcm vertically and 100 \u03bcm horizontally from the tip. For the small molecules anthracene and rhodamine 6G, the wire was cut and affixed to a metal target using double-sided conductive tape and analyzed by LDI using a commercial laser desorption time-of-flight mass spectrometer. Approximately 100 fg of material was ablated from each of the 1 \u03bcm ablation spots and transferred with approximately 3% efficiency. For larger polypeptide molecules angiotensin II and bovine insulin, the captured material was dissolved in saturated matrix solution and deposited on a target for MALDI analysis.<\/p>\n<\/div><\/div>\n\n\n\n

<\/div>
<\/div><\/div>
\n
\"\"
AFM image of ablation crater of an insulin thin film and MALDI mass spectrum of the collected material<\/figcaption><\/figure>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n
<\/div>
<\/div><\/div>
\n
<\/div>
<\/div><\/div>
\n
\"\"
Atomic force microscope with pulsed laser ablation and sample capture<\/figcaption><\/figure><\/div>\n<\/div><\/div>\n\n\n\n
<\/div>
<\/div><\/div>
\n
\"\"
Atomic force microscope stage with capture wire for tip enhanced laser ablation<\/figcaption><\/figure><\/div>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":693,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,5,80],"tags":[11,135,77],"class_list":["post-2128","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lsu","category-publication","category-tela","tag-asms","tag-lsu","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2128","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2128"}],"version-history":[{"count":3,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2128\/revisions"}],"predecessor-version":[{"id":2131,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2128\/revisions\/2131"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/693"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2128"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2128"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2128"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}