{"id":2123,"date":"2016-01-20T11:46:31","date_gmt":"2016-01-20T17:46:31","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2123"},"modified":"2024-02-20T09:34:49","modified_gmt":"2024-02-20T15:34:49","slug":"laser-desorption-sample-transfer-for-gas-chromatography-mass-spectrometry","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2016\/01\/laser-desorption-sample-transfer-for-gas-chromatography-mass-spectrometry\/","title":{"rendered":"Laser desorption sample transfer for gas chromatography\/mass spectrometry"},"content":{"rendered":"\n
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C.A. Seneviratne, S. Ghorai, K.K. Murray, “Laser desorption sample transfer for gas chromatography\/mass spectrometry,” Rapid Commun. Mass Spectrom.<\/em> 30<\/strong> (2016) 89\u201394. doi:10.1002\/rcm.7419<\/a>.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

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Abstract<\/strong><\/em>
Rationale:<\/em> Ambient mass spectrometry can detect small molecules directly, but complex mixtures can be a challenge. We have developed a method that incorporates small molecule separation based on laser desorption with capture on a solid\u2010phase microextraction (SPME) fiber for injection into a gas chromatography\/mass spectrometry (GC\/MS) system.
Methods<\/em>: Samples on a metal target were desorbed by a 3 \u00b5m mid\u2010infrared laser focused to a 250 \u00b5m spot and 1.2 mJ pulse energy. The desorbed material was aspirated into a metal tube suspended 1 mm above the laser spot and captured on a SPME fiber. The collected material was injected into a GC\/MS instrument for analysis.
Results<\/em>: We have developed a versatile approach for ambient laser desorption sampling onto SPME for GC\/MS analysis. The performance of the laser desorption SPME capture GC\/MS system was demonstrated for small molecule standards, a mixture of nitroaromatic explosives, and collected cigarette smoke.
Conclusions<\/em>: The utility of ambient laser desorption sampling onto SPME for GC\/MS was demonstrated. The performance of the method was evaluated by preparing calibration standards of caffeine over a range from 200 to 1000 ng. Laser desorption ambient sampling of complex mixtures was accomplished using SPME GC\/MS. <\/p>\n<\/div><\/div>\n\n\n\n

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Schematic representation of the experimental configuration for laser desorption sample transfer to SPME fiber. The heated transfer line is held 1 mm above the sample surface and the SPME fiber is inserted into a tee in the tube and exposed to the flow.<\/figcaption><\/figure>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":995,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[84,79,134,5],"tags":[135,174,77],"class_list":["post-2123","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-forensics","category-last","category-lsu","category-publication","tag-lsu","tag-paper","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2123","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2123"}],"version-history":[{"count":4,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2123\/revisions"}],"predecessor-version":[{"id":2127,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2123\/revisions\/2127"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/995"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2123"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2123"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2123"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}