{"id":2093,"date":"2019-07-19T17:04:02","date_gmt":"2019-07-19T22:04:02","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2093"},"modified":"2024-02-20T09:34:48","modified_gmt":"2024-02-20T15:34:48","slug":"a-nanoparticle-co-matrix-for-multiple-charging-in-maldi-imaging-of-tissue","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2019\/07\/a-nanoparticle-co-matrix-for-multiple-charging-in-maldi-imaging-of-tissue\/","title":{"rendered":"A Nanoparticle Co-matrix for Multiple Charging in MALDI Imaging of Tissue"},"content":{"rendered":"\n
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B. Banstola, K.K. Murray, “A Nanoparticle Co-matrix for Multiple Charging in MALDI Imaging of Tissue,” Rapid Commun. Mass Spectrom.<\/em> 16<\/strong> (2019) 12. doi:10.1002\/rcm.8424<\/a>.<\/p>\n\n\n\n

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Abstract<\/strong>
Rationale:<\/em> A two\u2010component matrix of 2\u2010nitrophloroglucinol (2\u2010NPG) and silica nanoparticles was used for matrix\u2010assisted laser desorption ionization (MALDI) mass spectrometry imaging of high\u2010charge\u2010state biomolecules in tissue. Potential advantages include increased effective mass range and efficiency of fragmentation.
Methods:<\/em> A mixture of 2\u2010NPG matrix and silica nanoparticles was applied to cyrosectioned 10\u2009\u03bcm thick mouse brain tissue. The mixture was pipetted onto the tissue for profiling and sprayed for tissue imaging. MALDI images were obtained under high vacuum in a commercial time\u2010of\u2010flight mass spectrometer.
Results: <\/em>The combined 2\u2010NPG and nanoparticle matrix produced highly charged ions from tissue with high\u2010vacuum MALDI. Nanoparticles of 20, 70, 400, and 1000\u2009nm in diameter were tested, the 20\u2009nm particles producing the highest charge states. Images of mouse brain tissue obtained from highly charged ions show similar spatial localization.
Conclusions: <\/em>The combined 2\u2010NPG and nanoparticle matrix produces highly charged ions from tissue through a mechanism that may rely on the high surface area of the particles which can dry the tissue, and their ability to bind analyte molecules thereby assisting in crystal formation and production of multiply charged ions on laser irradiation.<\/p>\n<\/div><\/div>\n\n\n\n

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\"Nanoparticle
Images of consecutive mouse brain tissue sections obtained from 8565 m\/z and its multiply charged ions with a) 2-NPG, and b) 2-NPG\/nanoparticle matrix.<\/figcaption><\/figure>\n<\/div><\/div>\n<\/div><\/div>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":2098,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,83,5],"tags":[15,135,100,9,101,174,77],"class_list":["post-2093","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lsu","category-mai","category-publication","tag-imaging","tag-lsu","tag-mai","tag-maldi","tag-nanoparticle","tag-paper","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2093","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2093"}],"version-history":[{"count":4,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2093\/revisions"}],"predecessor-version":[{"id":2717,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2093\/revisions\/2717"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/2098"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2093"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2093"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2093"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}