{"id":2088,"date":"2019-01-19T16:54:17","date_gmt":"2019-01-19T22:54:17","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=2088"},"modified":"2024-02-20T09:34:48","modified_gmt":"2024-02-20T15:34:48","slug":"tip-enhanced-laser-ablation-and-capture-of-dna","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2019\/01\/tip-enhanced-laser-ablation-and-capture-of-dna\/","title":{"rendered":"Tip-enhanced laser ablation and capture of DNA"},"content":{"rendered":"\n
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F. Cao, F. Donnarumma, K.K. Murray, Tip-enhanced laser ablation and capture of DNA, Appl. Surf. Sci.<\/em> 476<\/strong> (2019) 658\u2013662. doi:10.1016\/j.apsusc.2019.01.104<\/a>.<\/p>\n<\/div><\/div>\n\n\n\n

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Abstract: Tip-enhanced laser ablation was used to extract DNA plasmid for polymerase chain reaction (PCR) amplification. A 532\u202fnm nanosecond laser was directed onto a gold coated atomic force microscopy (AFM) tip 10\u202fnm above a sample surface to ablate a 7.1\u202fkbp green fluorescent protein (GFP) plasmid DNA sample on a glass coverslip. The ablated material was captured on a metal ribbon 300\u202f\u00b5m above the sample surface. The ablation craters had diameters from 1 to 2\u202f\u00b5m and an average volume of 0.14\u202f\u00b5m3. PCR and nested PCR were employed for the amplification of the ablated DNA. The quantity of sample from each ablation crater for PCR amplification was 20\u202fag.<\/p>\n<\/div><\/div>\n\n\n\n

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\"Tip-enhanced
The proposed technology allows topographical imaging with atomic force microscopy (AFM) and extraction of DNA via tip-enhanced laser ablation using the same tip. Plasmid DNA is imaged with a gold coated tip and extracted using a pulsed laser with a sampling size of 1\u202f\u00b5m. The captured DNA can be amplified by polymerase chain reaction (PCR) and nested PCR.<\/figcaption><\/figure>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":2090,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,5,80],"tags":[64,89,63,10,135,174,77],"class_list":["post-2088","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lsu","category-publication","category-tela","tag-ablation","tag-dna","tag-genomics","tag-laser","tag-lsu","tag-paper","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2088","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=2088"}],"version-history":[{"count":2,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2088\/revisions"}],"predecessor-version":[{"id":2091,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/2088\/revisions\/2091"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/2090"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=2088"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=2088"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=2088"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}