{"id":1998,"date":"2005-01-01T21:06:25","date_gmt":"2005-01-02T03:06:25","guid":{"rendered":"https:\/\/kermitmurray.com\/murray\/?p=1998"},"modified":"2019-08-16T14:23:14","modified_gmt":"2019-08-16T19:23:14","slug":"direct-coupling-of-polymer-based-microchip-electrophoresis-to-online-maldi-ms-using-a-rotating-ball-inlet","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2005\/01\/direct-coupling-of-polymer-based-microchip-electrophoresis-to-online-maldi-ms-using-a-rotating-ball-inlet\/","title":{"rendered":"Direct coupling of polymer-based microchip electrophoresis to online MALDI-MS using a rotating ball inlet"},"content":{"rendered":"\n<div class=\"wp-block-caxton-grid relative\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-columns caxton-grid-block\" style=\"padding-top:0;padding-left:0;padding-bottom:0;padding-right:0;grid-template-columns:repeat(12, 1fr)\" data-tablet-css=\"padding-left:em;padding-right:em;\" data-mobile-css=\"padding-left:em;padding-right:em;\">\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 12\"><div class=\"absolute absolute--fill\"><div class=\"cover bg-center absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p>H. Musyimi, J. Guy, D.A. Narcisse, S.A. Soper, K.K. Murray, Direct coupling of polymer-based microchip electrophoresis to online MALDI-MS using a rotating ball inlet, <em>Electrophoresis<\/em>. <strong>26<\/strong> (2005) 4703\u20134710. doi:<a href=\"https:\/\/doi.org\/10.1002\/elps.200500317\">10.1002\/elps.200500317<\/a>.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Abstract<\/h4>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright is-resized\"><a href=\"https:\/\/kermitmurray.com\/murray\/research\/maldi-microfluidics\/rotating-ball-inlet\/\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/kermitmurray.com\/murray\/wp-content\/uploads\/2011\/02\/rotating-ball-inlet.jpg\" alt=\"Rotating ball inlet with capillary electrophoresis microfluidic chip.\" class=\"wp-image-758\" width=\"480\" height=\"360\" srcset=\"https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2011\/02\/rotating-ball-inlet.jpg 640w, https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2011\/02\/rotating-ball-inlet-300x225.jpg 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><\/a><figcaption>Rotating ball inlet with capillary electrophoresis microfluidic chip.<\/figcaption><\/figure><\/div>\n\n\n\n<p>We report on the coupling of a polymer-based microfluidic chip to a MALDI-TOF MS using a rotating ball interface. The microfluidic chips were fabricated by micromilling a mold insert into a brass plate, which was then used for replicating polymer microparts via hot embossing. Assembly of the chip was accomplished by thermally annealing a cover slip to the embossed substrate to enclose the channels. The linear separation channel was 50 microm wide, 100 microm deep, and possessed an 8 cm effective length separation channel with a double-T injector (V(inj) = 10 nL). The exit of the separation channel was machined to allow direct contact deposition of effluent onto a specially constructed rotating ball inlet to the mass spectrometer. Matrix addition was accomplished in-line on the surface of the ball. The coupling utilized the ball as the cathode transfer electrode to transport sample into the vacuum for desorption with a 355 nm Nd:YAG laser and analyzed on a TOF mass spectrometer. The ball was cleaned online after every rotation. The ability to couple poly(methylmethacrylate) microchip electrophoresis devices for the separation of peptides and peptide fragments produced from a protein digest with subsequent online MALDI MS detection was demonstrated.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter is-resized\"><a href=\"https:\/\/kermitmurray.com\/murray\/research\/maldi-microfluidics\/ball-closeup\/\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/kermitmurray.com\/murray\/wp-content\/uploads\/2011\/02\/Ball-Closeup.jpg\" alt=\"Rotating ball inlet detail\" class=\"wp-image-759\" width=\"600\" height=\"450\" srcset=\"https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2011\/02\/Ball-Closeup.jpg 800w, https:\/\/kermitmurray.com\/research\/wp-content\/uploads\/2011\/02\/Ball-Closeup-300x225.jpg 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/a><figcaption>Rotating ball inlet detail<\/figcaption><\/figure><\/div>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":758,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,85,5],"tags":[135,77,142],"class_list":["post-1998","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lsu","category-microfluidic","category-publication","tag-lsu","tag-publication","tag-rotating-ball","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/1998","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=1998"}],"version-history":[{"count":2,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/1998\/revisions"}],"predecessor-version":[{"id":2368,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/1998\/revisions\/2368"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/758"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=1998"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=1998"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=1998"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}