{"id":1770,"date":"2019-03-12T08:57:52","date_gmt":"2019-03-12T13:57:52","guid":{"rendered":"https:\/\/kermitmurray.com\/research\/?p=1770"},"modified":"2024-02-20T09:34:48","modified_gmt":"2024-02-20T15:34:48","slug":"rna-sampling-from-tissue-sections-using-infrared-laser-ablation","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2019\/03\/rna-sampling-from-tissue-sections-using-infrared-laser-ablation\/","title":{"rendered":"RNA Sampling from Tissue Sections using Infrared Laser Ablation"},"content":{"rendered":"\n
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K. Wang, F. Donnarumma, S.W. Herke, C. Dong, P.F. Herke, K.K. Murray, RNA sampling from tissue sections using infrared laser ablation, Anal. Chim. Acta.<\/em> 1063<\/strong> (2019) 91\u201398. doi:10.1016\/j.aca.2019.02.054<\/a>.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

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\"RNA
RNA Sampling from Tissue Sections using Infrared Laser Ablation<\/figcaption><\/figure><\/div>\n\n\n\n

RNA was obtained from discrete locations of frozen rat brain tissue sections through infrared (IR) laser ablation using a 3-\u03bcm wavelength in transmission geometry. The ablated plume was captured in a microcentrifuge tube containing RNAse-free buffer and processed using a commercial RNA purification kit. RNA transfer efficiency and integrity were evaluated based on automated electrophoresis in microfluidic chips. Reproducible IR-laser ablation of intact RNA was demonstrated with purified RNA at laser fluences of 3-5 kJ\/m2<\/sup> (72\u00b112% transfer efficiency) and with tissue sections at a laser fluence of 13 kJ\/m2<\/sup> (79\u00b114% transfer efficiency); laser energies were attenuated \u223c20% by the soda-lime glass slides used to support the samples. RNA integrity from tissue ablation was >90% of its original RIN value (\u223c7) and the purified RNA was sufficiently intact for conversion to cDNA and subsequent qPCR assay.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":1771,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[79,134,5],"tags":[64,63,8,10,135,174,77],"class_list":["post-1770","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-last","category-lsu","category-publication","tag-ablation","tag-genomics","tag-infrared","tag-laser","tag-lsu","tag-paper","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/1770","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=1770"}],"version-history":[{"count":9,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/1770\/revisions"}],"predecessor-version":[{"id":2230,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/1770\/revisions\/2230"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/1771"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=1770"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=1770"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=1770"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}