{"id":1706,"date":"2018-06-28T11:23:51","date_gmt":"2018-06-28T16:23:51","guid":{"rendered":"https:\/\/kermitmurray.com\/research\/?p=1706"},"modified":"2024-02-20T09:34:48","modified_gmt":"2024-02-20T15:34:48","slug":"infrared-laser-ablation-sampling-coupled-with-data-independent-high-resolution-uplc-im-msms-for-tissue-analysis","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2018\/06\/infrared-laser-ablation-sampling-coupled-with-data-independent-high-resolution-uplc-im-msms-for-tissue-analysis\/","title":{"rendered":"Infrared laser ablation sampling coupled with data independent high resolution UPLC-IM-MS\/MS for tissue analysis"},"content":{"rendered":"\n
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M.E. Pettit, F. Donnarumma, K.K. Murray, T. Solouki, Infrared laser ablation sampling coupled with data independent high resolution UPLC-IM-MS\/MS for tissue analysis, Anal. Chim. Acta. 1034 (2018) 102\u2013109. doi:10.1016\/j.aca.2018.06.066<\/a>.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

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Infrared laser ablation sampling coupled with data independent high resolution\nUPLC-IM-MS\/MS for tissue analysis\nDOI: 10.1016\/j.aca.2018.06.066<\/figcaption><\/figure><\/div>\n\n\n\n

Infrared laser ablation microsampling was used with data-dependent acquisition (DDA) and ion mobility-enhanced data-independent acquisition (HDMSE<\/sup>) for mass spectrometry based bottom-up proteomics analysis of rat brain tissue. Results from HDMSE<\/sup> and DDA analyses of the 12 laser ablation sampled tissue sections showed that HDMSE<\/sup> consistently identified approximately seven times more peptides and four times more proteins than DDA. To evaluate the impact of ultra-performance liquid chromatography (UPLC) peak congestion on HDMSE<\/sup> and DDA analysis, whole tissue digests from rat brain were analyzed at six different UPLC separation times. Analogous to results from laser ablated samples, HDMSE<\/sup> analyses of whole tissue digests yielded about four times more proteins identified than DDA for all six UPLC separation times.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":1707,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[79,134,5],"tags":[64,8,10,135,174,77],"class_list":["post-1706","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-last","category-lsu","category-publication","tag-ablation","tag-infrared","tag-laser","tag-lsu","tag-paper","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/1706","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=1706"}],"version-history":[{"count":7,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/1706\/revisions"}],"predecessor-version":[{"id":2637,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/1706\/revisions\/2637"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/1707"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=1706"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=1706"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=1706"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}