{"id":149,"date":"2012-03-27T10:35:07","date_gmt":"2012-03-27T15:35:07","guid":{"rendered":"https:\/\/kermitmurray.com\/research\/?p=149"},"modified":"2019-08-03T16:11:16","modified_gmt":"2019-08-03T21:11:16","slug":"infrared-laser-ablation-sample-transfer-for-maldi-imaging","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2012\/03\/infrared-laser-ablation-sample-transfer-for-maldi-imaging\/","title":{"rendered":"Infrared Laser Ablation Sample Transfer for MALDI Imaging"},"content":{"rendered":"\n
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S.-G. Park, K.K. Murray, “Infrared laser ablation sample transfer for MALDI imaging,” Anal. Chem.<\/em> 84<\/strong> (2012) 3240\u20133245. doi:10.1021\/ac3006704<\/a>.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

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Abstract<\/em>: An infrared laser was used to ablate material from tissue sections under ambient conditions for direct collection on a matrix assisted laser desorption ionization (MALDI) target. A 10 \u03bcm thick tissue sample was placed on a microscope slide and was mounted tissue-side down between 70 and 450 \u03bcm from a second microscope slide. The two slides were mounted on a translation stage, and the tissue was scanned in two dimensions under a focused mid-infrared (IR) laser beam to transfer material to the target slide via ablation. After the material was transferred to the target slide, it was analyzed using MALDI imaging using a tandem time-of-flight mass spectrometer. Images were obtained from peptide standards for initial optimization of the system and from mouse brain tissue sections using deposition either onto a matrix precoated target or with matrix addition after sample transfer and compared with those from standard MALDI mass spectrometry imaging. The spatial resolution of the transferred material is approximately 400 \u03bcm. Laser ablation sample transfer provides several new capabilities not possible with conventional MALDI imaging including (1) ambient sampling for MALDI imaging, (2) area to spot concentration of ablated material, (3) collection of material for multiple imaging analyses, and (4) direct collection onto nanostructure assisted laser desorption ionization (NALDI) targets without blotting or ultrathin sections.<\/p>\n<\/div><\/div>\n\n\n\n

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LSU spelled with the peptide bradykinin transferred by IR laser ablation<\/figcaption><\/figure>\n\n\n\n
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Schematic of the laser ablation sample transfer system for MALDI imaging.<\/figcaption><\/figure>\n\n\n\n
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MALDI images of mouse brain sections (a) standard MALDI, (b) IR laser transfer to a matrix film, and (c) IR laser transfer with subsequent matrix addition.<\/figcaption><\/figure>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":635,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[79,134,5],"tags":[135,77],"class_list":["post-149","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-last","category-lsu","category-publication","tag-lsu","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/149","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=149"}],"version-history":[{"count":5,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/149\/revisions"}],"predecessor-version":[{"id":2175,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/149\/revisions\/2175"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/635"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=149"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=149"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=149"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}