{"id":118,"date":"2011-03-01T14:53:39","date_gmt":"2011-03-01T20:53:39","guid":{"rendered":"https:\/\/kermitmurray.com\/research\/?p=118"},"modified":"2019-08-03T16:11:16","modified_gmt":"2019-08-03T21:11:16","slug":"a-solid-phase-bioreactor-with-continuous-sample-deposition-for-matrix-assisted-laser-desorptionionization-time-of-flight-mass-spectrometry","status":"publish","type":"post","link":"https:\/\/kermitmurray.com\/research\/2011\/03\/a-solid-phase-bioreactor-with-continuous-sample-deposition-for-matrix-assisted-laser-desorptionionization-time-of-flight-mass-spectrometry\/","title":{"rendered":"A solid-phase bioreactor with continuous sample deposition for matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry"},"content":{"rendered":"\n
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J. Lee, S.A. Soper, K.K. Murray, “A solid-phase bioreactor with continuous sample deposition for matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry,” Rapid Commun. Mass Spectrom.<\/em> 25<\/strong> (2011) 693\u2013699. doi:10.1002\/rcm.4921<\/a>.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

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Abstract<\/em>: We report the development of a solid-phase proteolytic digestion and continuous deposition microfluidic chip platform for low volume fraction collection and off-line matrix-assisted laser desorption\/ionization time-of-flight (MALDI-TOF) mass spectrometry. Tryptic peptides were formed in an on-chip bioreactor and continuously deposited onto a MALDI target plate using a motor-driven xyz stage. The bioreactor consisted of a 4\u2009cm\u2009\u00d7\u2009200\u2009\u00b5m\u2009\u00d7\u200950\u2009\u00b5m microfluidic channel with covalently immobilized trypsin on an array of 50\u2009\u00b5m diameter micropost structures with a 50\u2009\u00b5m edge-to-edge inter-post spacing. A 50\u2009\u00b5m i.d. capillary tube was directly attached to the end of the bioreactor for continuous sample deposition. The MALDI target plate was modified by spin-coating a nitrocellulose solution containing a MALDI matrix on the surface prior to effluent deposition. Protein molecular weight standards were used for evaluating the performance of the digestion and continuous deposition system. Serpentine sample traces 200\u2009\u00b5m wide were obtained with a 30\u2009fmol\/mm quantity deposition rate and a 3.3\u2009nL\/mm volumetric deposition rate.<\/p>\n<\/div><\/div>\n\n\n\n

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A schematic of different deposition modes using the PMMA microfluidic chip and immobilized trypsin bioreactor: (a) continuous deposition mode and (b) spot deposition mode. The channel measured 40 mm x 200 mm x 50 mm and had an array of 50 mm diameter posts with a 50 mm inter-post spacing.<\/figcaption><\/figure>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":631,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[134,85,5],"tags":[135,22,18,77],"class_list":["post-118","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lsu","category-microfluidic","category-publication","tag-lsu","tag-microfluidics","tag-proteomics","tag-publication","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/118","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/comments?post=118"}],"version-history":[{"count":8,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/118\/revisions"}],"predecessor-version":[{"id":2119,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/posts\/118\/revisions\/2119"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media\/631"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/media?parent=118"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/categories?post=118"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kermitmurray.com\/research\/wp-json\/wp\/v2\/tags?post=118"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}