{"id":2499,"date":"2022-12-16T20:48:38","date_gmt":"2022-12-17T02:48:38","guid":{"rendered":"https:\/\/kermitmurray.com\/msblog\/?page_id=2499"},"modified":"2022-12-25T11:28:00","modified_gmt":"2022-12-25T17:28:00","slug":"reddit-r-proteomics","status":"publish","type":"page","link":"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-proteomics\/","title":{"rendered":"Reddit r\/proteomics"},"content":{"rendered":"\n<div class=\"wp-block-caxton-grid relative\"><div class=\"absolute absolute--fill\"><div class=\"absolute absolute--fill cover bg-center\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-columns caxton-grid-block\" style=\"padding-top:0;padding-left:0;padding-bottom:0;padding-right:0;grid-template-columns:repeat(12, 1fr)\" data-tablet-css=\"padding-left:em;padding-right:em;\" data-mobile-css=\"padding-left:em;padding-right:em;\">\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 8\"><div class=\"absolute absolute--fill\"><div class=\"absolute absolute--fill cover bg-center\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p><a href=\"https:\/\/www.reddit.com\/r\/proteomics\/\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>\/r\/proteomics\/<\/strong><\/a><\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 4\"><div class=\"absolute absolute--fill\"><div class=\"absolute absolute--fill cover bg-center\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p><strong><a href=\"https:\/\/www.reddit.com\/r\/proteomics.rss\" target=\"_blank\" rel=\"noreferrer noopener\">RSS<\/a><\/strong><\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n<ul class=\"has-dates has-authors has-excerpts wp-block-rss\"><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1ub30u1\/i_got_tired_of_clicking_through_alphafoldboltz\/'>I got tired of clicking through AlphaFold\/Boltz output folders, so I wrote a thing that dumps them all into one HTML report<\/a><\/div><time datetime=\"2026-06-20T17:58:15-05:00\" class=\"wp-block-rss__item-publish-date\">June 20, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/No-Cartoonist1757<\/span><div class=\"wp-block-rss__item-excerpt\">submitted by \/u\/No-Cartoonist1757 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1u92ncd\/protein_purification_using_spin_concentrators_to\/'>Protein purification: Using spin concentrators to get filtrate instead?<\/a><\/div><time datetime=\"2026-06-18T10:31:37-05:00\" class=\"wp-block-rss__item-publish-date\">June 18, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Groundbreaking-Pen85<\/span><div class=\"wp-block-rss__item-excerpt\">submitted by \/u\/Groundbreaking-Pen85 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1u8m2km\/dda_vs_dia_for_low_abdundance_proteins\/'>DDA VS DIA for low abdundance proteins<\/a><\/div><time datetime=\"2026-06-17T20:44:48-05:00\" class=\"wp-block-rss__item-publish-date\">June 17, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Crazy-Tax-1320<\/span><div class=\"wp-block-rss__item-excerpt\">Hello, for low-abundance proteins in IP samples, which do you think is better overall: DDA or DIA? submitted by \/u\/Crazy-Tax-1320 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1u7xub2\/anyone_has_experience_with_hispur_ninta_spin\/'>Anyone has experience with HisPur Ni-NTA Spin Columns and protein purification from plants?<\/a><\/div><time datetime=\"2026-06-17T02:36:28-05:00\" class=\"wp-block-rss__item-publish-date\">June 17, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Groundbreaking-Pen85<\/span><div class=\"wp-block-rss__item-excerpt\">submitted by \/u\/Groundbreaking-Pen85 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1u70hsu\/tmt_efficiency_issues_with_di_gly_peptides\/'>TMT Efficiency issues with Di Gly peptides<\/a><\/div><time datetime=\"2026-06-16T02:05:47-05:00\" class=\"wp-block-rss__item-publish-date\">June 16, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Crazy-Tax-1320<\/span><div class=\"wp-block-rss__item-excerpt\">I started with approximately 1 mg of peptides prior to diGly enrichment and used a TMT-after elution workflow. For the sample that gave 98% labeling efficiency, the enriched peptides were labeled directly after elution. (IP) For the sample that gave 84% labeling efficiency, the peptides went through Zip cleaning and loaded on the machine to check for Di Gly sites before TMT tagging What could be the reason for low efficiency and i noticed lower TMT efficiency for flow through IPs sample that were also TMT tagged Has anyone encountered something similar when performing TMT labeling on diGly-enriched peptides? Also [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1u6vm9v\/coolgene_bio_community_coolgene_community_open\/'>CoolGene Bio Community: CoolGene Community Open Event (By 7\/31)<\/a><\/div><time datetime=\"2026-06-15T22:31:36-05:00\" class=\"wp-block-rss__item-publish-date\">June 15, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Pleasant-Wonder-1665<\/span><div class=\"wp-block-rss__item-excerpt\">submitted by \/u\/Pleasant-Wonder-1665 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1u6txw8\/skills_to_get_a_job_after_phd_in_proteomics_usa\/'>Skills to get a Job After PhD in Proteomics USA<\/a><\/div><time datetime=\"2026-06-15T21:26:49-05:00\" class=\"wp-block-rss__item-publish-date\">June 15, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Proteo-Freak973<\/span><div class=\"wp-block-rss__item-excerpt\">Hola Redditians !! Context is; I&#039;m currently doing my 3.5 years PhD in proteomics in a CRO in NZ mostly with the plant-based system, but my whole bachelors and masters background is in pharmacy, and I also have some idea of mammalian proteomics, drug discovery pathways and so on. Problem is; I&#039;m worried about the job market in USA, People advising me PostDoc is necessary you&#039;ll not get a job, But I&#039;m tired of academics and I want stability in an industry Question is; What will you suggest me as an action from the first year, so I&#039;ll directly land [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1u3vdon\/free_evosep_webinar_assay_development_for\/'>Free Evosep Webinar: Assay Development for Translation and Research Applications<\/a><\/div><time datetime=\"2026-06-12T13:31:36-05:00\" class=\"wp-block-rss__item-publish-date\">June 12, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/EvosepBio<\/span><div class=\"wp-block-rss__item-excerpt\">Hi everyone, We\u2019d like to share an upcoming webinar that may be of interest to the community in here! On June 18, 2026 (16:00 CEST \/ 10:00 EDT \/ 07:00 PST), we are hosting a session on \u201cAssay Development for Translational and Research Applications.\u201d Speaker: Giles Drinkwater (Researcher, OXcan Analytics) \u2014 \u201cRobust Data from Evosep Eno Coupled to Exploris 480 for the Development of Diagnostic Assays.\u201d Machine learning-derived diagnostic models have enormous potential, but their success depends on the quality and robustness of the underlying data. Giles will discuss OXcan Analytics\u2019 workflow for generating reliable proteomics data from digested plasma [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1u3vbsh\/proteomics_kits\/'>proteomics kits<\/a><\/div><time datetime=\"2026-06-12T13:29:28-05:00\" class=\"wp-block-rss__item-publish-date\">June 12, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Disastrous-Offer220<\/span><div class=\"wp-block-rss__item-excerpt\">hey! our lab currently uses the preomics it-nhs kits for our proteomics process. they are currently back ordered and going to take 4-6 weeks to come in. our team is looking at the thermoscientific easypep MS sample prep kits. does anyone know if they are comparable? thank you!! submitted by \/u\/Disastrous-Offer220 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1u3eco5\/chromalyst_alpha_seeking_feedback_on\/'>Chromalyst Alpha: Seeking Feedback on Evidence-Based Peak Tiering for LC-MS\/MS<\/a><\/div><time datetime=\"2026-06-11T23:02:56-05:00\" class=\"wp-block-rss__item-publish-date\">June 11, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/BizMaker_2504<\/span><div class=\"wp-block-rss__item-excerpt\">I\u2019m building Chromalyst (chromalyst.io\/dashboard)\u2014 an evidence-based peak intelligence layer for chromatographic workflows. It uses machine learning and physics-informed validation to detect, tier, and explain chromatographic peaks, helping scientists separate high-confidence peaks from noise, artifacts, and ambiguous candidates. Chromalyst is currently in alpha \/ early validation, with an initial focus on LC-MS\/DIA data. Your feedback, especially from folks working with real chromatographic workflows, around peak quality, and usability, will help us improve the product. submitted by \/u\/BizMaker_2504 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1u1kpgk\/ptms_proteoforms_profiling\/'>PTMs \/ Proteoforms profiling<\/a><\/div><time datetime=\"2026-06-09T22:57:11-05:00\" class=\"wp-block-rss__item-publish-date\">June 9, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Mean_Dragonfly_3068<\/span><div class=\"wp-block-rss__item-excerpt\">submitted by \/u\/Mean_Dragonfly_3068 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tzldrt\/please_review_my_lfq_qc_tool\/'>Please Review My LFQ QC Tool<\/a><\/div><time datetime=\"2026-06-07T19:22:20-05:00\" class=\"wp-block-rss__item-publish-date\">June 7, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Weak-Indication-7166<\/span><div class=\"wp-block-rss__item-excerpt\">Hey peeps! So I have basically developed and published a small QC tool for the LFQ (DDA) Data. It&#039;s available as both GUI (for Windows) and CLI (for Linux). I would appreciate if you checkout and lemme know about it. Plus it generates both sample wise and combined samples MultiQC type reports. This tool is to simply know your data before you run the downstream analysis. https:\/\/github.com\/thy-sanjay\/QCPROT Do checkout and lemme know, thanks in advance \ud83d\ude09 submitted by \/u\/Weak-Indication-7166 [link] [comments]<\/div><\/li><\/ul>\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\"\/>\n\n\n\n<h4 class=\"wp-block-heading\">Related Feeds<\/h4>\n\n\n<ul class=\"su-siblings\"><li class=\"page_item page-item-2733\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-analyticalchemistry\/\">Reddit r\/analyticalchemistry<\/a><\/li>\n<li class=\"page_item page-item-2707\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-genomics\/\">Reddit r\/genomics<\/a><\/li>\n<li class=\"page_item page-item-2473\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-massspectrometry\/\">Reddit r\/massspectrometry<\/a><\/li>\n<li class=\"page_item page-item-2705\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-metabolomics\/\">Reddit r\/metabolomics<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Related Feeds<\/p>\n","protected":false},"author":1,"featured_media":2498,"parent":2738,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2499","page","type-page","status-publish","has-post-thumbnail","hentry","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2499","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/comments?post=2499"}],"version-history":[{"count":4,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2499\/revisions"}],"predecessor-version":[{"id":2745,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2499\/revisions\/2745"}],"up":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2738"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/media\/2498"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/media?parent=2499"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}