{"id":2499,"date":"2022-12-16T20:48:38","date_gmt":"2022-12-17T02:48:38","guid":{"rendered":"https:\/\/kermitmurray.com\/msblog\/?page_id=2499"},"modified":"2022-12-25T11:28:00","modified_gmt":"2022-12-25T17:28:00","slug":"reddit-r-proteomics","status":"publish","type":"page","link":"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-proteomics\/","title":{"rendered":"Reddit r\/proteomics"},"content":{"rendered":"\n<div class=\"wp-block-caxton-grid relative\"><div class=\"absolute absolute--fill\"><div class=\"absolute absolute--fill cover bg-center\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-columns caxton-grid-block\" style=\"padding-top:0;padding-left:0;padding-bottom:0;padding-right:0;grid-template-columns:repeat(12, 1fr)\" data-tablet-css=\"padding-left:em;padding-right:em;\" data-mobile-css=\"padding-left:em;padding-right:em;\">\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 8\"><div class=\"absolute absolute--fill\"><div class=\"absolute absolute--fill cover bg-center\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p><a href=\"https:\/\/www.reddit.com\/r\/proteomics\/\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>\/r\/proteomics\/<\/strong><\/a><\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 4\"><div class=\"absolute absolute--fill\"><div class=\"absolute absolute--fill cover bg-center\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p><strong><a href=\"https:\/\/www.reddit.com\/r\/proteomics.rss\" target=\"_blank\" rel=\"noreferrer noopener\">RSS<\/a><\/strong><\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n<ul class=\"has-dates has-authors has-excerpts wp-block-rss\"><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1ttfrpu\/what_peptides_are_best_to_help_me_finish_my_phd\/'>What peptides are best to help me finish my PhD?<\/a><\/div><time datetime=\"2026-06-01T03:08:15-05:00\" class=\"wp-block-rss__item-publish-date\">June 1, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Mental-Ferret-1018<\/span><div class=\"wp-block-rss__item-excerpt\">I heard this is a subreddit for peptide enthusiasts. Please send me the number of your peptide dealer along with your thoughts and prayers! submitted by \/u\/Mental-Ferret-1018 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tq5rzk\/help_build_a_protein_design_computer\/'>HELP: Build a protein design computer<\/a><\/div><time datetime=\"2026-05-28T14:46:35-05:00\" class=\"wp-block-rss__item-publish-date\">May 28, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Dizzy-Version7196<\/span><div class=\"wp-block-rss__item-excerpt\">Hello guys, I am working in a pharmacy lab in Korea, and we don&#039;t have a computer cluster. PI needs me to give her the spec. of a computer that can run protein and antibody in silicon design software locally (such as Boltzgen, RFantibody, RFdiffusion) I am not a computer major. I asked ChatGPT and got some specs, but I want to make sure by finding advice from the person who actually runs that software. Because we need to run thousands of samples on Boltzgen or RFantibody, running them on the VM or a pay website is not financially efficient [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tpqxtn\/low_tmt_labelling_efficiency_troubleshooting\/'>Low TMT labelling efficiency troubleshooting<\/a><\/div><time datetime=\"2026-05-28T02:50:58-05:00\" class=\"wp-block-rss__item-publish-date\">May 28, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/thesymbiont<\/span><div class=\"wp-block-rss__item-excerpt\">We&#039;re experiencing somewhat low TMTpro labelling efficiency (&gt;97% N-terminus, but only ~80% K labelling based on psm) but can&#039;t identify a cause. These are SP3 digests of whole-cell lysates, with FragPipe as the search. We&#039;re using a peptide:TMT ratio of between 1:1 and 1:4, incubated for 1 hour at room tempearture in 100mM HEPES pH 8.3. The reaction is quenched with 0.4% hydroxylamine final for 15 min at room temp. Any common errors that we might be making to look out for? submitted by \/u\/thesymbiont [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tpiq3b\/zuckerbergs_biohub_unveils_ai_world_model_of\/'>Zuckerberg&#039;s Biohub unveils AI &quot;world model&quot; of proteins<\/a><\/div><time datetime=\"2026-05-27T21:08:46-05:00\" class=\"wp-block-rss__item-publish-date\">May 27, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Vailhem<\/span><div class=\"wp-block-rss__item-excerpt\">submitted by \/u\/Vailhem [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tp5rbz\/how_to_correct_for_signal_drift_due_to_ion_source\/'>How to correct for signal drift due to ion source contamination in MALDI MSI?<\/a><\/div><time datetime=\"2026-05-27T13:34:12-05:00\" class=\"wp-block-rss__item-publish-date\">May 27, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Xierrax<\/span><div class=\"wp-block-rss__item-excerpt\">Hi, I&#039;m fairly new to MALDI-MSI bioinformatics and running into a signal drift problem I can&#039;t find much literature on. I&#039;m comparing two large tumor tissue sections on a single slide. The MALDI instrument measures them sequentially \u2014 tissue A first, then tissue B. I have two slides total with the measurement order swapped on the second slide (B first, A second), specifically to counterbalance any order effect. The same tissue measured first vs. second shows clear and substantial intensity differences in a lot of samples, the second-measured tissue showing lower signal, likely due to gradual ion source contamination during [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tncxye\/looking_to_build_a_computational_protein\/'>Looking to build a Computational Protein Engineering group!<\/a><\/div><time datetime=\"2026-05-25T15:43:50-05:00\" class=\"wp-block-rss__item-publish-date\">May 25, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/PatientSpecialist669<\/span><div class=\"wp-block-rss__item-excerpt\">Hey guys! I have been exploring the field of computational protein engineering for the past year and have slowly started to fall in love with it. I have been trying to find the place to meet new people in the fiels to share and discuss ideas and possible projects. One thing I\u2019ve been struggling with, though, is finding a solid group of people who aren\u2019t just learning this stuff, but are excited to learn and build something together. I\u2019m really interested in bringing together a small community of like-minded people who want to collaborate on projects, share ideas, and actually [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tkiqd1\/enabling_gpu_on_spectronaut_202\/'>Enabling GPU on Spectronaut 20.2<\/a><\/div><time datetime=\"2026-05-22T13:17:11-05:00\" class=\"wp-block-rss__item-publish-date\">May 22, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/CommandOwn1557<\/span><div class=\"wp-block-rss__item-excerpt\">Hello all, any experience enabling GPU for inference analysis in Spectronaut 20.2? Does it make the analysis faster? Thanks submitted by \/u\/CommandOwn1557 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tk9c2c\/noncptac_sources_of_mass_spec_proteomics_with\/'>Non-CPTAC sources of Mass Spec proteomics with WSI\/H&amp;E<\/a><\/div><time datetime=\"2026-05-22T05:23:34-05:00\" class=\"wp-block-rss__item-publish-date\">May 22, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/TetralogyofFallot_<\/span><div class=\"wp-block-rss__item-excerpt\">Hi all, was wondering if anyone could direct me to some datasets of matches whole slide image and proteomics to validate a deep learning model. Thanks! submitted by \/u\/TetralogyofFallot_ [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tjfso5\/free_evosep_webinar_preasms_2026_sample_prep_kits\/'>Free Evosep Webinar: Pre-ASMS 2026 &#8211; Sample Prep Kits &amp; Sample Prep Station<\/a><\/div><time datetime=\"2026-05-21T09:58:49-05:00\" class=\"wp-block-rss__item-publish-date\">May 21, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/EvosepBio<\/span><div class=\"wp-block-rss__item-excerpt\">Hi everyone, We\u2019d like to share an upcoming webinar that may be of interest to the community in here! On May 28, 2026 (16:00 CEST \/ 10:00 EDT \/ 07:00 PST), we are hosting a session on \u201cPreparing for Next Generation Evosep Proteomics.\u201d In this webinar, we\u2019ll be giving an exclusive first look at Evosep\u2019s upcoming standardized sample preparation kits and our brand-new Evosep sample preparation station, designed to help scale and standardize proteomics workflows for research and drug development. Speakers: Dorte Bekker-Jensen (VP Product &amp; R&amp;D, Evosep) and Nicolai Bache (Chief Strategic Officer, Evosep) \u2014 \u201cPreparing for Next Generation [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tj395u\/how_is_mass_spectrometry_proteomics_learning_it\/'>How is mass spectrometry proteomics learning it and as a career?<\/a><\/div><time datetime=\"2026-05-20T23:46:46-05:00\" class=\"wp-block-rss__item-publish-date\">May 20, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Better_Personality70<\/span><div class=\"wp-block-rss__item-excerpt\">I was accepted to an analytical chemistry PhD. I did a little bit of research in environmental analytical labs in undergrad, and have been working in industry these past few years. For a year I worked in an analytical environmental chemistry lab running lots of LC\/GC\/ a little GCMS, and the past couple years have been working as a field service engineer on dissolution and physical testing systems for big pharma. I really would like to get involved with more advanced instrumentation and become an expert at it to get to a higher role in industry, either in pharma or [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tfu3rz\/tool_go_quickly_check_quality_of_multiple_raw\/'>Tool go quickly check quality of multiple raw files<\/a><\/div><time datetime=\"2026-05-17T16:20:55-05:00\" class=\"wp-block-rss__item-publish-date\">May 17, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/DrDad19<\/span><div class=\"wp-block-rss__item-excerpt\">Hello everybody, I work at a proteomics core and one issue we&#039;re trying to solve is a faster or preferably automated way to do a quick quality check of multiple raw files at once, before we search them. What currently happens is someone opens each raw file in qual browser to make sure the chromatography looks consistent between runs, before giving the &quot;all good&quot; to the data analysis person to search them. I appreciate any suggestions. submitted by \/u\/DrDad19 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1tf7rgx\/orbitrap_lumos_xcalibur_is_dia_supposed_to_be\/'>Orbitrap Lumos Xcalibur. Is DIA supposed to be separate from MS1 experiment?<\/a><\/div><time datetime=\"2026-05-16T22:39:09-05:00\" class=\"wp-block-rss__item-publish-date\">May 16, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Crazy-Tax-1320<\/span><div class=\"wp-block-rss__item-excerpt\">Hi everyone, I\u2019m setting up a DIA method on an Orbitrap Lumos in Xcalibur. I want to run MS1 full scan + DIA in the same method, but I\u2019m running into an issue: -I can add MS1 in Experiment 1 -I can only add DIA scans in Experiment 2 -If I try to place DIA under Experiment 1, it doesn\u2019t work or doesn\u2019t seem supported Is DIA actually supposed to be a separate experiment (Experiment 2) in the scan tree, or should it be possible to integrate it under Experiment 1? Any help would be appreciated submitted by \/u\/Crazy-Tax-1320 [link] [&hellip;]<\/div><\/li><\/ul>\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\"\/>\n\n\n\n<h4 class=\"wp-block-heading\">Related Feeds<\/h4>\n\n\n<ul class=\"su-siblings\"><li class=\"page_item page-item-2733\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-analyticalchemistry\/\">Reddit r\/analyticalchemistry<\/a><\/li>\n<li class=\"page_item page-item-2707\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-genomics\/\">Reddit r\/genomics<\/a><\/li>\n<li class=\"page_item page-item-2473\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-massspectrometry\/\">Reddit r\/massspectrometry<\/a><\/li>\n<li class=\"page_item page-item-2705\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-metabolomics\/\">Reddit r\/metabolomics<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Related Feeds<\/p>\n","protected":false},"author":1,"featured_media":2498,"parent":2738,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2499","page","type-page","status-publish","has-post-thumbnail","hentry","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2499","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/comments?post=2499"}],"version-history":[{"count":4,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2499\/revisions"}],"predecessor-version":[{"id":2745,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2499\/revisions\/2745"}],"up":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2738"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/media\/2498"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/media?parent=2499"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}