{"id":2499,"date":"2022-12-16T20:48:38","date_gmt":"2022-12-17T02:48:38","guid":{"rendered":"https:\/\/kermitmurray.com\/msblog\/?page_id=2499"},"modified":"2022-12-25T11:28:00","modified_gmt":"2022-12-25T17:28:00","slug":"reddit-r-proteomics","status":"publish","type":"page","link":"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-proteomics\/","title":{"rendered":"Reddit r\/proteomics"},"content":{"rendered":"\n<div class=\"wp-block-caxton-grid relative\"><div class=\"absolute absolute--fill\"><div class=\"absolute absolute--fill cover bg-center\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-columns caxton-grid-block\" style=\"padding-top:0;padding-left:0;padding-bottom:0;padding-right:0;grid-template-columns:repeat(12, 1fr)\" data-tablet-css=\"padding-left:em;padding-right:em;\" data-mobile-css=\"padding-left:em;padding-right:em;\">\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 8\"><div class=\"absolute absolute--fill\"><div class=\"absolute absolute--fill cover bg-center\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p><a href=\"https:\/\/www.reddit.com\/r\/proteomics\/\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>\/r\/proteomics\/<\/strong><\/a><\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-caxton-section relative\" style=\"grid-area:span 1\/span 4\"><div class=\"absolute absolute--fill\"><div class=\"absolute absolute--fill cover bg-center\" style=\"background-color:;background-image:linear-gradient( );\"><\/div><div class=\"absolute absolute--fill\" style=\"background-color:;background-image:linear-gradient( );opacity:1;\"><\/div><\/div><div class=\"relative caxton-section-block\" style=\"padding-top:5px;padding-left:5px;padding-bottom:5px;padding-right:5px\" data-mobile-css=\"padding-left:1em;padding-right:1em;\" data-tablet-css=\"padding-left:1em;padding-right:1em;\">\n<p><strong><a href=\"https:\/\/www.reddit.com\/r\/proteomics.rss\" target=\"_blank\" rel=\"noreferrer noopener\">RSS<\/a><\/strong><\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n<ul class=\"has-dates has-authors has-excerpts wp-block-rss\"><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1srlur2\/free_evosep_webinar_nextgen_host_cell_protein\/'>Free Evosep Webinar: Next-Gen Host Cell Protein Analysis in Biopharma<\/a><\/div><time datetime=\"2026-04-21T12:13:36-05:00\" class=\"wp-block-rss__item-publish-date\">April 21, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/EvosepBio<\/span><div class=\"wp-block-rss__item-excerpt\">Hi everyone, We\u2019d like to share an upcoming webinar that may be of interest to the community in here! On April 30, 2026 (16:00 CEST \/ 10:00 EDT), we are hosting a session on \u201cNext-Gen HCP Analysis in Biopharma.\u201d Speakers: Somar Khalil (GSK, Principal Scientist, Analytical Research &amp; Development) \u2014 \u201cProspective ICH Q2(R2)-Aligned Total-Error Validation of Label-Free Untargeted Proteomics for Host Cell Protein Quantification in Biotherapeutics.\u201d Untargeted proteomics enables quantitative determination of host cell proteins (HCPs) in biotherapeutics, yet no workflow has been validated under ICH Q2(R2) for regulated quality control. Somar will present a prospective validation of label-free untargeted [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1sqetky\/can_someone_explain_a_proteomic_analysis_using\/'>Can someone explain a Proteomic analysis using SAPs table to me<\/a><\/div><time datetime=\"2026-04-20T04:16:28-05:00\" class=\"wp-block-rss__item-publish-date\">April 20, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Tschelinaaw<\/span><div class=\"wp-block-rss__item-excerpt\">Hi! I\u2019m currently working on a presentation about the Harbin skull for university and I\u2019ve hit a wall with one of the studies There\u2019s a table on proteomic analysis using SAPs (Single Amino Acid Polymorphisms) and I don\u2019t fully understand how the classification works. I get the general idea that amino acids are being compared across Harbin, Denisovans, Neanderthals, etc., but I dont quite know how to really read or explain the table. Does anyone here have experience with proteomics or paleo-genetic analysis and could maybe explain this? I\u2019d really appreciate it If you want to take a look at [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1snow47\/has_anyone_played_with_calcium_to_improve\/'>Has anyone played with Calcium to improve trypsinization efficiency? Any tips? Would calcium interfere with Lys-C steps.<\/a><\/div><time datetime=\"2026-04-17T03:05:18-05:00\" class=\"wp-block-rss__item-publish-date\">April 17, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/bluemooninvestor<\/span><div class=\"wp-block-rss__item-excerpt\">Currently I am using TEAB 100mM 8.5 with 1% SDC with trypsin. Any advice on whether adding a bit of Cacl2 would help with missed cleavages? I am at around 20%. Promega Sequencing grade trypsin. submitted by \/u\/bluemooninvestor [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1snhbs3\/proteolysis_optimization_strategies\/'>Proteolysis Optimization Strategies<\/a><\/div><time datetime=\"2026-04-16T21:38:51-05:00\" class=\"wp-block-rss__item-publish-date\">April 16, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Proteo-Freak973<\/span><div class=\"wp-block-rss__item-excerpt\">Will someone please suggest, how do you optimize the proteolysis aka Digestion for the proteomics approaches? Is there any rule or way for trials and errors? submitted by \/u\/Proteo-Freak973 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1snh9q1\/chemical_phosphorylation\/'>Chemical Phosphorylation<\/a><\/div><time datetime=\"2026-04-16T21:36:38-05:00\" class=\"wp-block-rss__item-publish-date\">April 16, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Proteo-Freak973<\/span><div class=\"wp-block-rss__item-excerpt\">Can someone guide me how to induce phosphorylation in the sample and how to optimize that particular reaction taking proteomics into consideration? Because there will be some chemicals pH in the reaction which will be not suitable for the proteomics workflow submitted by \/u\/Proteo-Freak973 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1skw2od\/whats_the_best_lysis_buffer_for_adult_rat_cardiac\/'>What&#039;s the best lysis buffer for adult rat cardiac fibroblasts for mass spec?<\/a><\/div><time datetime=\"2026-04-14T02:38:49-05:00\" class=\"wp-block-rss__item-publish-date\">April 14, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/bigby_wolf150<\/span><div class=\"wp-block-rss__item-excerpt\">submitted by \/u\/bigby_wolf150 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1shg4hx\/peptidomicsprotemics_quality_control\/'>Peptidomics\/Protemics Quality Control<\/a><\/div><time datetime=\"2026-04-10T07:58:33-05:00\" class=\"wp-block-rss__item-publish-date\">April 10, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/fnepo18<\/span><div class=\"wp-block-rss__item-excerpt\">submitted by \/u\/fnepo18 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1sgi6v2\/bruker_vs_thermo_real_world_experience\/'>Bruker vs Thermo &#8211; real world experience?<\/a><\/div><time datetime=\"2026-04-09T06:52:45-05:00\" class=\"wp-block-rss__item-publish-date\">April 9, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/letsplayhungman<\/span><div class=\"wp-block-rss__item-excerpt\">Looking to buy a new MS for proteomics of all shapes and kinds (at a service center &#8211; so everything from lysates to single cell to xl-ms). The leading contenders these days are the timsTOF AIP and the astral (or astral zoom). I\u2019ve seen a bunch of data produced by the companies themselves and I\u2019ve spoken to users of both and they both look great. The issue is that these days all the heavy users and proteomics veterans have very strong feelings for one of the companies before we even start looking at the MS itself &#8211; generally the feelings [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1sg0m7d\/looking_to_convert_very_large_raw_files_to_mzml\/'>Looking to convert very large raw files to mzml<\/a><\/div><time datetime=\"2026-04-08T18:06:42-05:00\" class=\"wp-block-rss__item-publish-date\">April 8, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/dampcroutons<\/span><div class=\"wp-block-rss__item-excerpt\">Hi! I&#039;m a high school student working on a research project using raw spectra data from SCOPE2 (Slavov Lab). However, I am running into an issue when I am attempting to convert these raw files to mzML format. I have a MacBook, and I am trying to use ProteoWizard to convert these files. I downloaded a Windows VM to operate ProteoWizard (it doesn&#039;t work on Mac). However, I keep getting an error that the files are exceeding the size limit, so I am unable to do this conversion. Could someone please suggest what I should do in order to successfully [&hellip;]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1sec8v2\/enrichment_platform_for_microbial\/'>Enrichment platform for microbial proteins\/peptides online?<\/a><\/div><time datetime=\"2026-04-06T21:30:22-05:00\" class=\"wp-block-rss__item-publish-date\">April 6, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/FactorAgreeable7518<\/span><div class=\"wp-block-rss__item-excerpt\">Hi everyone, I\u2019m conducting research on gut microbiota in tryptic digest samples collected from resected tissues of individuals with inflammatory bowel disease (IBD). I\u2019ve identified several peptides and subsequent proteins. I\u2019m curious if anyone is aware of an online platform (GUI) that allows to input these protein IDs or peptide lists to analyze their biological enrichment, similar to what we do with String or David enrichment tools. I\u2019ve tried using these platforms, but none of them recognizes bacterial protein IDs. Could you please advise me on the best platform to use for this purpose? submitted by \/u\/FactorAgreeable7518 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1sawdwm\/mean_imputation\/'>Mean imputation<\/a><\/div><time datetime=\"2026-04-02T22:50:34-05:00\" class=\"wp-block-rss__item-publish-date\">April 2, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/No_Newt4239<\/span><div class=\"wp-block-rss__item-excerpt\">Opinion: when mean imputing would you split controls and cases and then impute to preserve signal or keep them together to prevent over fitting (I know mean imputing isn\u2019t the best but for the sake of this question) submitted by \/u\/No_Newt4239 [link] [comments]<\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/www.reddit.com\/r\/proteomics\/comments\/1s8fmpw\/proteomic_ruler_applicable_to_dia_data\/'>Proteomic ruler applicable to DIA data?<\/a><\/div><time datetime=\"2026-03-31T06:26:19-05:00\" class=\"wp-block-rss__item-publish-date\">March 31, 2026<\/time> <span class=\"wp-block-rss__item-author\">by \/u\/Expensive-Painter-18<\/span><div class=\"wp-block-rss__item-excerpt\">Has any1 tried using DIA NN for Proteomics ruler based copy number estimation? I know orginal paper is based on MaxQuant and Perseus based plugin but in general, DIA data should be more quantitative and less susceptible to missing values\/peptides hence though to use Proteomics ruler concept here. Also, label free quantitation should accurate in DIA mode. Any suggestions\/comments? Is my logic right? submitted by \/u\/Expensive-Painter-18 [link] [comments]<\/div><\/li><\/ul>\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\"\/>\n\n\n\n<h4 class=\"wp-block-heading\">Related Feeds<\/h4>\n\n\n<ul class=\"su-siblings\"><li class=\"page_item page-item-2733\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-analyticalchemistry\/\">Reddit r\/analyticalchemistry<\/a><\/li>\n<li class=\"page_item page-item-2707\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-genomics\/\">Reddit r\/genomics<\/a><\/li>\n<li class=\"page_item page-item-2473\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-massspectrometry\/\">Reddit r\/massspectrometry<\/a><\/li>\n<li class=\"page_item page-item-2705\"><a href=\"https:\/\/kermitmurray.com\/msblog\/links\/social-feeds\/reddit-feeds\/reddit-r-metabolomics\/\">Reddit r\/metabolomics<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Related Feeds<\/p>\n","protected":false},"author":1,"featured_media":2498,"parent":2738,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2499","page","type-page","status-publish","has-post-thumbnail","hentry","entry"],"_links":{"self":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2499","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/comments?post=2499"}],"version-history":[{"count":4,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2499\/revisions"}],"predecessor-version":[{"id":2745,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2499\/revisions\/2745"}],"up":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/pages\/2738"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/media\/2498"}],"wp:attachment":[{"href":"https:\/\/kermitmurray.com\/msblog\/wp-json\/wp\/v2\/media?parent=2499"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}